A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf

Gengan, Robert Moonsamy; Anand, Krishnan; Phulukdaree, Alisa; Chuturgoon, Anil A.

doi:10.1016/j.colsurfb.2012.12.044

Cited by 178 publications

(84 citation statements)

References 37 publications

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“…Nanosilvers either chemically or biologically synthesized have unequalled properties, and have gained prominence because of their anticancer, antimicrobial, antibacterial, larvicidal, antioxidant antiparasitic, anticoagulant, thrombolytic and antifungal properties Jayaseelan et al 2011;Sukirtha et al 2012;Zahir and Rahuman 2012;Raliya et al 2015;Azeez et al 2016;Lateef et al 2016a, b, c, d;Ojo et al 2016). Due to these properties nanosilvers have been used in therapeutics, and applied in manufacturing of plastics, textile, healthcare products and electrical appliances (Gengan et al 2013;Schlich and Hund-Rinke 2015;Lateef et al 2016e).…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

2017

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This study investigates the influence of different concentrations of AgNPs biologically synthesized using pod extract of Cola nitida on antioxidant activity, phenolic contents, flavonoid contents and compositions of Amaranthus caudatus L. AgNPs of 25, 50, 75, 100 and 150 ppm were utilized in growing A. caudatus while water was used as control. Delayed germination for two days was observed for A. caudatus grown with 150 ppm of AgNPs, while others showed no difference. There were 43.3, 38.7, 26.7 and 6.48% improvements in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity of A. caudatus grown with 25, 50, 75 and 100 ppm of AgNPs, respectively, compared to control. Antioxidant activity of A. caudatus grown with AgNPs reduced with increase in the concentrations of AgNPs. A. caudatus grown with 50 ppm of AgNPs was the most potent with the least IC 50 of 0.67 mg/ml. Significant improvements obtained for phenolic and flavonoid contents grown with AgNPs were concentration dependent. Enhancements of 21.9, 68.19, and 1.98% in phenolic contents were achieved in treatments with 25, 50 and 75 ppm AgNPs, respectively, while 32.58, 35.80, and 7.20% improvement in flavonoids were obtained for 25, 50 and 100 ppm treatments, respectively. Kaempferol and quercetin were the most abundant flavonoids in A. caudatus treated with 50 ppm of AgNPs, showing the highest flavonoid composition. This further confirms A. caudatus grown with 50 ppm of AgNPs as the most potent. This study has shown that concentration-dependent AgNPs can be used to boost antioxidant activity and phytochemical contents of vegetables.

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Section: Introductionmentioning

confidence: 99%

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

2017

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“…1). Same type of results was observed in Albizia adianthifolia leaf mediated synthesis of silver nanoparticles [37]. FTIR spectrum of synthesized SNPs was carried out to know the possible biomolecules responsible for capping and stabilization of nanoparticles.…”

Section: Resultsmentioning

confidence: 85%

Leaf Assisted Green Synthesis of Silver Nanoparticles from Syzygium Alternifolium (Wt.) Walp. Characterization and Antimicrobial Studies

Yugandhar¹,

Savithramma²

2015

Nano BioMed ENG

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Development of environmentally benign methods for synthesis of nanoparticles is an evolving important branch of nanotechnology. In the present study we report a green method for synthesis of stable silver nanoparticles (SNPs) from aqueous leaf extract of Syzygium alternifolium an endemic medicinal plant to Southern Eastern Ghats. These green synthesized nanoparticles are characterized by using UV-VIS spectroscopy, FTIR, XRD, AFM, SEM with EDAX and TEM. The colour change from yellow to grey is observed upon synthesis and 448 nm peak was obtained from UV-VIS spectroscopic analysis. FTIR spectroscopic studies confirms that phenols and proteins of leaf extract is main responsible for capping and stabilization of these SNPs. Crystallographic studies from XRD indicated the SNPs are crystalline in nature and owing 44 nm size. High resolution and magnification studies with AFM, SEM and TEM analysis revealed that the nanoparticles are spherical in shape having the size range from 7 to 44 nm. EDAX pattern of synthesized SNPs showed 47.28 weight percentage of Ag metal in the sample indicate the purity of sample. Further, the antimicrobial studies of these green synthesized SNPs show high toxicity towards different bacterial and fungal isolates. The results revealed that the selected medicinal plant possess the potentiality towards the synthesis of narrow range nanoparticles also combat with the pathogens.

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“…It was reported that although AgNPs exhibited significant cytotoxicity against NSCLC A549 cells, they showed no cytotoxicity toward normal healthy peripheral lymphocytes (PLs). 17 Greulich et al reported silver ions were more cytotoxic toward bacteria and human cells than AgNPs. 25 The effects of AgNPs are distinct from those of silver ions in inducing neurotoxicity through oxidative stress in rat cerebral astrocytes.…”

Section: Introductionmentioning

confidence: 99%

Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

He¹,

Du²,

Ma³

et al. 2016

IJN

153

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Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention.

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A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf

Cited by 178 publications

References 37 publications

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

Leaf Assisted Green Synthesis of Silver Nanoparticles from Syzygium Alternifolium (Wt.) Walp. Characterization and Antimicrobial Studies

Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

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