Aberrant expression of p53, p16INK4a and Ki-67 as basic biomarker for malignant progression of oral leukoplakias

Nasser, Wasim; Flechtenmacher, Christa; Holzinger, Dana; Hofele, Christof; Bosch, Franz X.

doi:10.1111/j.1600-0714.2011.01026.x

Cited by 73 publications

(71 citation statements)

References 27 publications

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“…21,22,23 In 11 premalignant cases, p53 positivity was found in 72.72% cases with an increase in suprabasal positivity as the grade of dysplasia increased. Such pattern of staining was also observed by Cruz et al, 24,25 Kerdpon et al, 26 Vered et al 27 and Nasser et al 28 These investigators also found that the p53 expression pattern was significantly related to the development of carcinoma. No statistical significance was found between histological grades, possibly due to small number of cases studied.…”

Section: Discussionsupporting

confidence: 69%

P53 Expression in Benign, Premalignant and Malignant Lesions of Oral Cavity

Pradhan¹,

Choudhury²,

Dash³

et al. 2016

jemds

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BACKGROUNDOral carcinogenesis is a multistep process. The premalignant oral lesions, which develop in the epithelial surface due to chronic exposure to various carcinogens such as tobacco, alcohol, betel chewing and HPV infections get transformed to squamous cell carcinoma in genetically predisposed person. Genetic alterations most commonly associated with oral carcinogenesis is p53 tumour suppressor gene mutation.The objectives of this study are,  To find out the incidence of various oral squamous epithelial lesions and to evaluate and compare immunohistochemical expression of p53.  To assess the degree of p53 staining intensity from dysplasia to malignancy in differentiating benign and malignant lesions. MATERIALS AND METHODSIn this prospective study, histopathological examination and immunohistochemical staining was performed to see the p53 expression in these oral epithelial lesions. RESULTSImmunohistochemical expression of p53 observed in 52 cases of oral epithelial lesions consisting of 8 benign, 11 pre-malignant and 33 squamous cell carcinomas. Out of all, 39 cases were showed p53 positivity. The dysplastic lesions account for 11 cases of which p53 positivity seen in 8 (72.72%) cases; p53 immunoexpression was found positive in 26 (78.78%) cases of Squamous Cell Carcinoma (SCC). The staining intensity increased from basal to suprabasal region with increase in grade of dysplasias. CONCLUSIONSquamous cell carcinoma was found to be the commonest oral malignant epithelial lesion, in which p53 immunoexpression was found in 78.78% cases. Combined histological analysis with p53 immunoexpression could be a useful and simple molecular marker to detect the possibility of transformation from a premalignant to malignant lesion of oral epithelium.

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Section: Discussionsupporting

confidence: 69%

P53 Expression in Benign, Premalignant and Malignant Lesions of Oral Cavity

Pradhan¹,

Choudhury²,

Dash³

et al. 2016

jemds

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“…The p53 protein is essential in protecting the cells against DNA damage induced directly and indirectly by UV radiation [1,12,18]. Immunohistochemical expression of p53 is largely accepted as a marker of malignant transformation, and its overexpression has been associated with malignant potential in potentially malignant lesions of the head and neck [23,24]. In the current study, p53 was present in AC, being slightly higher in AC with moderate/severe dysplasia group than in the no/mild dysplasia one.…”

Section: Discussionmentioning

confidence: 54%

Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer

et al. 2016

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This study aims to evaluate and verify the relationship between the immunoexpression of hMSH2, p53 and p21 in actinic cheilitis (AC) and lower lip squamous cell carcinoma (SCC) cases. Forty AC and 40 SCC cases were submitted to immunoperoxidase method and quantitatively analyzed. Expression was compared by Mann-Whitney test, Student t test or one-way ANOVA. To correlate the variables, Pearson's correlation coefficient was calculated. The expression of p53 and p21 showed no significant differences between histopathological grades of AC or lower lip SCC (p [ 0.05). Immunoexpression of p53 was higher in SCC than in AC (p \ 0.001), while p21 expression was more observed in AC when compared to SCC group (p = 0.006). The AC group revealed an inverse correlation between p53 and hMSH2 expression (r = -0.30, p = 0.006). Alterations in p53 and p21 expression suggest that these proteins are involved in lower lip carcinogenesis. Moreover, p53 and hMSH2 seem to be interrelated in early events of this process.

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“…Numerous studies have compared the biological properties of premalignant lesions with their malignant potential; to this end, the expression of some biomarkers in oral precancerous and cancerous lesions have been compared. 6,23,[25][26][27][28] The molecular events that cause a premalignant lesion to turn into a carcinoma are still unknown, and it is still impossible to predict which premalignant lesion will progress to carcinoma. 29 …”

Section: Resultsmentioning

confidence: 99%

The Evaluation of Ghrelin Expression In Oral Leukoplakia And Oral Squamous Cell Carcinoma: An Immunohistochemical Study

Fikirli¹,

Özeç²,

Eğılmez³

et al. 2017

EÜ Dişhek Fak Derg

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ÖZET AMAÇ: Biobelirteçler normal dokuda, prekanseröz lezyonlarda ve kanserli dokuda farklı miktarlarda bulunabilen proteinler veya genlerdir, biobelirteçlerin değerlendirilmesi daha etkin bir şekilde malign transformasyon öngörüsü yapılmasını sağlayabilir. Bu çalışmanın amacı normal oral epitel, oral lökoplaki ve oral skuamoz hücreli karsinomada (OSHK) ghrelin seviyelerinin araştırılarak karşılaştırılmasıdır. YÖNTEM: Patoloji bölümü arşivinden elde edilen toplam 55 adet daha önce tanısı konulmuş normal oral mukoza (n = 15), oral lökoplaki (n = 18) ve OSHK (n = 22) bloklarından deparafinize edilerek elde edilen preparatlar özel antikorlar kullanılarak immünhistokimyasal olarak boyanmış ve ghrelin seviyeleri değerlendirilmiştir. İmmünhistokimyasal boyamada ghrelin mevcudiyeti mikroskop altında 100 hücre değerlendirilerek sayısallaştırılmıştır. Ghrelin pozitif hücrelerde kahverengi sitoplazmik boyama görülmüştür. BULGULAR: Normal oral mukozada hücrelerinin % 64'ünde, oral lökoplaki hücrelerinin % 66'sında ghrelin pozitif boyama görülürken, OSHK'da bu boyama yalnızca hücrelerin % 8'inde olmuştur. Diğer gruplar ile karşılaştırıldığı zaman OSHK grubunda ghrelin pozitif boyalı hücre miktarının istatistiksel olarak anlamlı şekilde az olduğu görülmüştür (P < 0.05). SONUÇ: Normal oral mukoza ve oral lökoplaki ile karşılaştırıldığı zaman OSHK grubunda ghrelin seviyesinin daha az olduğu tespit edilmiştir. Oral lökoplaki ve normal oral mukoza gruplarının ghrelinin seviyelerinin benzer olduğu belirlenmiştir. Oral karsinogenezis ile birlikte oluşan ghrelin seviyesinde ki değişikliklerin artmış malign potansiyelin belirlenmesinde bir biobelirteç olabileceği düşünülmektedir.

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Aberrant expression of p53, p16INK4a and Ki-67 as basic biomarker for malignant progression of oral leukoplakias

Cited by 73 publications

References 27 publications

P53 Expression in Benign, Premalignant and Malignant Lesions of Oral Cavity

P53 Expression in Benign, Premalignant and Malignant Lesions of Oral Cavity

Correlation between cell cycle proteins and hMSH2 in actinic cheilitis and lip cancer

The Evaluation of Ghrelin Expression In Oral Leukoplakia And Oral Squamous Cell Carcinoma: An Immunohistochemical Study

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