“…The virilizing effect of the synthetic steroids has been widely examined in experimental animals by estimating various parameters such as anogenital distance in male and females (Revesz et al, 1960;Suchowsky and Junkmann, 1961;Scholer and deWachter, 1961;Falconi et al, 1961;Lerner et al, 1962), prostate weight (Hershberger et al, 1953;Sanders and Drill, 1956;Lister et al, 1959), seminal vesicle weight 1956; Lyster et al, 1959;Suchowsky and Junkmann, 1961) or anatomical changes of genitalia on transverse sections of female fetus of rat (Jost, 1961;Scholer et al, 1961;Suchowsky et al, 1961). However, the result obtained by the transverse section method does not necessarily parallel with the change in anogenital distance measured microscopically, and a misinterpretation is brought occasionally due to differing-section levels (Neumann and Junkmann, 1963 (Table 2).…”