Achieving Automated Organelle Biopsy on Small Single Cells Using a Cell Surgery Robotic System

Shakoor, Adnan; Xie, Mingyang; Luo, Tao; Shen, Yajing; Mills, James K.; Sun, Dong

doi:10.1109/tbme.2018.2885772

Cited by 47 publications

(38 citation statements)

References 51 publications

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“…These experiments illustrate the kind of tasks where the presented platform could be implemented. Possible biological applications that can benefit from the proposed platform include cell sorting, isolation, rotation, stimulation, 3D tomographic imaging, and can contribute to more complex tasks such as single-cell surgeries (e.g., nuclear transplantation, embryo micro-injections and polar-body biopsy [39,40]) or micro-assembling [41].…”

Section: Discussionmentioning

confidence: 99%

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

et al. 2019

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Single-cell manipulation is considered a key technology in biomedical research. However, the lack of intuitive and effective systems makes this technology less accessible. We propose a new tele–robotic solution for dexterous cell manipulation through optical tweezers. A slave-device consists of a combination of robot-assisted stages and a high-speed multi-trap technique. It allows for the manipulation of more than 15 optical traps in a large workspace with nanometric resolution. A master-device (6+1 degree of freedom (DoF)) is employed to control the 3D position of optical traps in different arrangements for specific purposes. Precision and efficiency studies are carried out with trajectory control tasks. Three state-of-the-art experiments were performed to verify the efficiency of the proposed platform. First, the reliable 3D rotation of a cell is demonstrated. Secondly, a six-DoF teleoperated optical-robot is used to transport a cluster of cells. Finally, a single-cell is dexterously manipulated through an optical-robot with a fork end-effector. Results illustrate the capability to perform complex tasks in efficient and intuitive ways, opening possibilities for new biomedical applications.

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Section: Discussionmentioning

confidence: 99%

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

et al. 2019

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“…Conventional methods to isolate individual organelles, such as micropipette aspiration and optical trapping of adherent cells, are low throughput (≈10 1 -10 2 organelles per hour), require a skilled experimentalist, and therefore do not scale well for the application to many organelles across many cells. [61,62] Several promising micro-and nano-devices have been developed in recent years to address these issues. For example, neurons can be cultured within microfluidic culture chambers that allow fluidic access specific to individual axons [33,34] (Figure 2a).…”

Section: Micro-and Nano-devices For Subcellular Isolation and Organelmentioning

confidence: 99%

Micro‐ and Nano‐Devices for Studying Subcellular Biology

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Yang²,

Kadam

et al. 2020

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Cells are complex machines whose behaviors arise from their internal collection of dynamically interacting organelles, supramolecular complexes, and cytoplasmic chemicals. The current understanding of the nature by which subcellular biology produces cell‐level behaviors is limited by the technological hurdle of measuring the large number (>103) of small‐sized (<1 μm) heterogeneous organelles and subcellular structures found within each cell. In this review, the emergence of a suite of micro‐ and nano‐technologies for studying intracellular biology on the scale of organelles is described. Devices that use microfluidic and microelectronic components for 1) extracting and isolating subcellular structures from cells and lysate; 2) analyzing the physiology of individual organelles; and 3) recreating subcellular assembly and functions in vitro, are described. The authors envision that the continued development of single organelle technologies and analyses will serve as a foundation for organelle systems biology and will allow new insight into fundamental and clinically relevant biological questions.

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“…Figure 5b illustrates the intracellular localization of JC-1 stained mitochondria. The mitochondrion contour was extracted by setting a threshold defined as Hue, Saturation and Value (HSV) range value [126]. Although Gauss [124], and Median filters [126] or morphological operations [123] was adopted to smoothen the sampled images in the aforementioned methods, most out-of-focus blurs and imaging noises were difficult to eliminate.…”

Section: Segmentationmentioning

confidence: 99%

“…The mitochondrion contour was extracted by setting a threshold defined as Hue, Saturation and Value (HSV) range value [126]. Although Gauss [124], and Median filters [126] or morphological operations [123] was adopted to smoothen the sampled images in the aforementioned methods, most out-of-focus blurs and imaging noises were difficult to eliminate. Direct segmentation with a threshold value or edge detectors on original sampled images is only suitable for the manipulations of large-sized objects, because it may erase useful information near real object boundaries and produce inaccurate contours and localization of targeted specimens.…”

Section: Segmentationmentioning

confidence: 99%

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

et al. 2020

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Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.

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Achieving Automated Organelle Biopsy on Small Single Cells Using a Cell Surgery Robotic System

Cited by 47 publications

References 51 publications

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

Micro‐ and Nano‐Devices for Studying Subcellular Biology

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

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