Activation of murine RAW264.7 macrophages by oligopeptides from sea cucumber (Apostichopus japonicus) and its molecular mechanisms

Cai, Nan; Luo, Wenqi; Yao, Lijun; Li, Xiuting; Wang, Zuzhe; Xu, Hong; Li, Hui; Hu, Zhangli; Bao, Wei-Yang; Xu, Xu

doi:10.1016/j.jff.2020.104229

Cited by 19 publications

(13 citation statements)

References 27 publications

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“…The mRNA levels of TNF-α, IL-1β, and IL-6 in RAW 264.7 cells were detected by quantitative reverse-transcription PCR in the present study; however, the production of these cytokines in the macrophage supernatant was not determined by ELISA. According to several studies, when RAW 264.7 cells were stimulated with test substances, there was a positive relationship between mRNA expression levels and the production of the cytokines, TNF-α, IL-1β, and IL-6 (Cai et al, 2020;Guo et al, 2020;Park et al, 2020;Fan et al, 2021;Feng et al, 2021). Therefore, in this study, the immune cells were assumed to have higher cytokine protein levels if the corresponding mRNA levels were higher.…”

Section: Discussionmentioning

confidence: 88%

Immune-enhancing effects of β-lactoglobulin glycated with lactose following in vitro digestion on cyclophosphamide-induced immunosuppressed mice

Chun

Lee

2022

Journal of Dairy Science

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β-Lactoglobulin (β-LG) is a major milk protein, making up more than 53% of the total whey proteins, and is seen as a valuable ingredient in food processing because of its high essential amino acid content and diverse functional applications. The Maillard reaction can occur during the storage and processing of food and generate various beneficial effects, including antiallergenicity, antioxidant, and immunomodulatory effects. The addition of an β-LG-lactose conjugate (LGL) produced by the Maillard reaction was shown to have a strong immune-enhancing effect, increasing both nitric oxide generation and cytokine expression through activation of RAW 264.7 cells, even after in vitro digestion. Furthermore, daily LGL administration resulted in the upregulation of several immune markers in a cyclophosphamide-induced immunosuppressive mouse model, indicating that this treatment stimulates multiple immune cells, including macrophages, natural killer cells, and lymphocytes, enhancing the proliferation and activation of both the innate and adaptive immune responses. Taken together, these findings indicate that consuming LGL on a regular basis can improve immunity by increasing the natural production of various immune cells.

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Section: Discussionmentioning

confidence: 88%

Immune-enhancing effects of β-lactoglobulin glycated with lactose following in vitro digestion on cyclophosphamide-induced immunosuppressed mice

Chun

Lee

2022

Journal of Dairy Science

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“…The levels of TNF-α and IL-6 in Raw264.7 macrophages was determined according to the method reported in ref with slight modifications. In brief, RAW264.7 cells (1 × 10 5 /well) were added to 6-well plates and induced using LPS (1 μg/mL).…”

Section: Methodsmentioning

confidence: 99%

Isolation, Identification, and Anti-Inflammatory Activity of Polysaccharides of Typha angustifolia

et al. 2021

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The present study aimed to purify, structurally characterize, and evaluate the anti-inflammatory activity of the polysaccharide extracted from Typha angustifolia. Two purified polysaccharides (PTA-1 and PTA-2) were obtained via DEAE-52 cellulose chromatography. Their structural characterizations and antioxidant activity were in vitro analyzed. To evaluate the anti-inflammatory activity of PTA-2, the levels of inflammatory cytokines, intracellular ROS production, and the inhibitory effects of the transcriptional activation of the nuclear factor kappa B (NF-κB) signaling pathway were determined. PTA-1 comprises glucose (100%) with α-(1 → 3) glycosidic bonds, and PTA-2 comprises glucose (66.7%) and rhamnose (33.3%) formed by β-(1 → 3) glycosidic bonds. PTA-1 and PTA-2 showed strong antioxidant activity in vitro. Moreover, PTA-2 intervention (50, 100, and 200 μg/mL) suppressed the production of inflammatory cytokines, the activation of NF-κB signaling, and reactive oxygen species production significantly. The results identified PTA-2 as a natural product that could be applied in anti-inflammatory drugs.

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“…In their study, the sea cucumber hydrolysate obtained by a mixture of various proteases significantly induced NO production and increased iNOS mRNA as well as protein expressions. 56 Meanwhile, SCPs activated the NF-κB and MAPKs signaling pathways in RAW264.7 cells against immune suppression. In addition to cell models, C57BL/6 and BALB/c mice have been used to determine the immune enhancement function of SCP.…”

Section: Production Of Sea Cucumber Peptidesmentioning

confidence: 99%

Production of Bioactive Peptides from Sea Cucumber and Its Potential Health Benefits: A Comprehensive Review

Sun

Liu

et al. 2022

J. Agric. Food Chem.

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Bioactive peptides from food have been widely studied due to their potential applications as functional foods and pharmaceuticals. Sea cucumber, a traditional tonic food, is characterized by high protein and low fat, thereby substrates are being studied to release sea cucumber peptides (SCPs). Although recent studies have shown that SCPs have various bioactive functions, there is no literature reviewing the development status of SCPs. In this review, we summarized the production of SCPs, including their purification and identification, then mainly focused on the comprehensive potential health benefits of SCP in vivo and in vitro, and finally discussed the challenge facing the development of SCPs. We found that SCPs have well-documented health benefits due to their antioxidation, anti-diabetes, ACE inhibitory, immunomodulatory, anti-cancer, anti-fatigue, anti-aging, neuroprotection, micromineral-chelating, etc. However, the structure−activity relationships of SCPs and the functional molecular mechanisms underlying their regulation in vivo need further investigation. Research on the safety of SCP and its potential regulation mechanism will contribute to transferring these findings into commercial applications. Hopefully, this review could promote the development and application of SCPs in further investigation and commercialization.

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Activation of murine RAW264.7 macrophages by oligopeptides from sea cucumber (Apostichopus japonicus) and its molecular mechanisms

Cited by 19 publications

References 27 publications

Immune-enhancing effects of β-lactoglobulin glycated with lactose following in vitro digestion on cyclophosphamide-induced immunosuppressed mice

Immune-enhancing effects of β-lactoglobulin glycated with lactose following in vitro digestion on cyclophosphamide-induced immunosuppressed mice

Isolation, Identification, and Anti-Inflammatory Activity of Polysaccharides of Typha angustifolia

Production of Bioactive Peptides from Sea Cucumber and Its Potential Health Benefits: A Comprehensive Review

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