Activation of Xenopus Eggs by the Kinase Inhibitor 6-DMAP Suggests a Differential Regulation of Cyclin B and p39mos Proteolysis

Bodart, Jean‐François; Béchard, David; Bertout, Marc; Gannon, Julian; Rousseau, Arlette; Vilain, Jean‐Pierre; Flament, Stéphane

doi:10.1006/excr.1999.4662

Cited by 22 publications

(15 citation statements)

References 39 publications

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“…Cytological Analysis-At the end of the maturation process, the position of the meiotic spindles was determined as described previously (23). Briefly, oocytes were fixed overnight, dehydrated in an ascending ethanol series, and placed overnight in butanol.…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of FTDP-17 tau Gene Mutations through Their Effects on Xenopus Oocyte Maturation

Delobel¹,

Flament²,

Hamdane³

et al. 2002

Journal of Biological Chemistry

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tau gene mutations cause frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). Here we have used Xenopus oocyte maturation as an indicator of microtubule function. We show that wildtype four-repeat Tau protein inhibits maturation in a concentration-dependent manner, whereas three-repeat Tau has no effect. Of the seven four-repeat Tau proteins with FTDP-17 mutations tested, five (G272V, ⌬K280, P301L, P301S, and V337M) failed to interfere significantly with oocyte maturation, demonstrating a greatly reduced ability to interact with microtubules. One mutant protein (R406W) almost behaved like wildtype Tau, and one (S305N) inhibited maturation more strongly than wild-type Tau. With the exception of R406W, wild-type Tau and all the mutants studied were similarly phosphorylated during the Xenopus oocyte maturation, and this was independent of their effects on this process. Data obtained with R406W and S305N may be related to charge changes (phosphorylation and basic amino acids). Our results demonstrate variable effects of FTDP-17 mutations on microtubules in an intact cell situation. Those findings establish Xenopus oocyte maturation as a system allowing the study of the functional effects of tau gene mutations in a quantitative manner.

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Section: Methodsmentioning

confidence: 99%

Functional Characterization of FTDP-17 tau Gene Mutations through Their Effects on Xenopus Oocyte Maturation

Delobel¹,

Flament²,

Hamdane³

et al. 2002

Journal of Biological Chemistry

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“…Sections are then stained with Nuclear Red to detect nuclei and chromosomes, and with picroindigocarmine, which reveals cytoplasmic structures (Bodart et al, 1999). This method enables the detection of spindle and condensed chromosomes, even if not located near the plasma membrane.…”

Section: Spindle Morphogenesis and Aneuploidy Detectionmentioning

confidence: 99%

Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

Ješeta¹,

Bodart²

2012

Aneuploidy in Health and Disease

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“…In this study, we optimized the conditions for parthenogenesis and cleavage of golden hamster oocytes. Previous studies have reported that parthenogenetic activation of oocytes from several species may be induced by chemical reagents, including calcium ionophore [3], strontium (Sr 2+ ) [4], ethanol [5], cycloheximide [6], 6-dimethylaminopurine (6-DMAP) [7], as well as by electrical stimulation [8]. Despite these findings, the activation and cleavage induction of oocytes remains one of the least efficient steps in the nuclear transplantation process.…”

Section: Introductionmentioning

confidence: 99%

Effects of Electrical Pulse and 6-DMAP on Cleavage of Golden Hamster Oocytes—Morphological and Phisiological Observations

Wang¹,

Jiang²,

Li³

2016

JFMK

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Abstract:The golden hamster is a well-established model system for studies of morphology, reproductive physiology, oncology, genetics and virology. The aim of this study was to establish experimental protocols necessary for cloning the golden hamster; we examined and optimized conditions for parthenogenesis and cleavage of its oocytes. We tested oocytes of different ages, including 15 h after Human chorionic gonadotropin (hCG), with two treatments: (1) an electrical pulse ranging from 10 to 600 V/mm and (2) incubation for 2 to 6 h in 2 mM 6-dimethylaminopurine (6-DMAP). These two conditions were tested both separately and in combination. We found that (i) in oocytes of different ages, cleavage exhibits a strength-dependent increase; (ii) 6-DMAP stimulates oocyte cleavage, but the cleavage rates are significantly low; and (iii) a combined treatment is more effective than a treatment with 6-DMAP alone, and is comparable to those achieved with high pulse stimuli. These results elucidate certain parameters important for the cloning of the golden hamster species.

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Activation of Xenopus Eggs by the Kinase Inhibitor 6-DMAP Suggests a Differential Regulation of Cyclin B and p39mos Proteolysis

Cited by 22 publications

References 39 publications

Functional Characterization of FTDP-17 tau Gene Mutations through Their Effects on Xenopus Oocyte Maturation

Functional Characterization of FTDP-17 tau Gene Mutations through Their Effects on Xenopus Oocyte Maturation

Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

Effects of Electrical Pulse and 6-DMAP on Cleavage of Golden Hamster Oocytes—Morphological and Phisiological Observations

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