Active chitinases in the apoplastic fluids of healthy white lupin (Lupinus albus L.) plants

Burzyński, Adam; Piślewska, Mariola; Wojtaszek, Przemysław

doi:10.1007/s11738-000-0005-6

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Both chitinase and glucanase have been shown to play a role in plant defence against pathogenic fungi, in white lupin as well as in other plant species (Vierheilig et al . 1994; Burzynski, Pislewska & Wojtaszek 2000; Tonon et al . 2002).…”

Section: Discussionmentioning

confidence: 99%

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition

Weisskopf

Abou-Mansour

Fromin

et al. 2006

Plant Cell & Environment

168

116

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White lupins ( Lupinus albus L.) respond to phosphate deficiency by producing special root structures called cluster roots. These cluster roots secrete large amounts of carboxylates into the rhizosphere, mostly citrate and malate, which act as phosphate solubilizers and enable the plant to grow in soils with sparingly available phosphate. The success and efficiency of such a P-acquisition strategy strongly depends on the persistence and stability of the carboxylates in the soil, a parameter that is influenced to a large extent by biodegradation through rhizosphere bacteria and fungi. In this study, we show that white lupin roots use several mechanisms to reduce microbial growth. The abundance of bacteria associated with cluster roots was decreased at the mature state of the cluster roots, where a burst of organic acid excretion and a drastic pH decrease is observed. Excretion of phenolic compounds, mainly isoflavonoids, induced fungal sporulation, indicating that vegetative growth, and thus potential citrate consumption, is reduced. In addition, the activity of two antifungal cell wall-degrading enzymes, chitinase and glucanase, were highest at the stage preceding the citrate excretion. Therefore, our results suggest that white lupin has developed a complex strategy to reduce microbial degradation of the phosphate-solubilizing agents.

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Section: Discussionmentioning

confidence: 99%

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition

Weisskopf

Abou-Mansour

Fromin

et al. 2006

Plant Cell & Environment

168

116

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show abstract

“…Chitinases are classified as PR proteins . They can degrade chitin, a major component of the fungal cell wall, inhibit growth of fungal pathogens and thus play an important role in the biocontrol of plant pathogenic fungi . Some forms of chitinase are synthesized constitutively in non‐stressed .…”

Section: Discussionmentioning

confidence: 99%

Comparative analyses of three legume species reveals conserved and unique root extracellular proteins

Liao

Hochholdinger

2012

Proteomics

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Increasing evidence suggests that root extracellular proteins are involved in interactions between roots and their soil environment. In the present study, exudates released by 6-day-old roots of the three legume species white lupin (Lupinus albus), soybean (Glycine max), and cowpea (Vigna sinensis) were collected under axenic conditions, and their constitutively secreted proteomes were analyzed. Between 42 and 93 unique root extracellular proteins with 2 or more different peptide fragments per protein were identified by LC-MS/MS. Functional annotation of these proteins classified them into 14-16 different functional categories. Among those 14 homologous proteins were identified in at least two legume species. Among the unique proteins, 58 in white lupin, 85 in soybean, and 31 in cowpea were specific for each plant species, and many of them were classified in the same functional categories. Interestingly, in contrast to soybean and cowpea, two protein bands of approximately 16 and 30 kDa were present on the SDS-PAGE gel of white lupin. The identification of these bands revealed a class III chitinase and a thaumatin-like protein. Both belong to the class of pathogenesis-related proteins. The results imply that root extracellular proteins play important roles in the cross-talk between plant roots and the rhizosphere.

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“…The stability of carboxylates in the soil is key to maintain P acquisition and counteract Al toxicity. This is achieved by a temporal regulation of the secretion of isoflavonoids, antifungal cell wall-degrading enzymes, chitinase and glucanase prior to OAs excretion, thus reducing microorganisms population and preventing OA degradation ( Neumann et al, 1999 ; Burzyński et al, 2000 ; Zhu et al, 2005 ).…”

Section: Role Of Rhizobia In Lupin Aluminum Tolerancementioning

confidence: 99%

Adaptive Mechanisms Make Lupin a Choice Crop for Acidic Soils Affected by Aluminum Toxicity

2022

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Almost half of the world’s agricultural soils are acidic, and most of them present significant levels of aluminum (Al) contamination, with Al3+ as the prevailing phytotoxic species. Lupin is a protein crop that is considered as an optimal alternative to soybean cultivation in cold climates. Lupins establish symbiosis with certain soil bacteria, collectively known as rhizobia, which are capable of fixing atmospheric nitrogen. Moreover, some lupin species, especially white lupin, form cluster roots, bottlebrush-like structures specialized in the mobilization and uptake of nutrients in poor soils. Cluster roots are also induced by Al toxicity. They exude phenolic compounds and organic acids that chelate Al to form non-phytotoxic complexes in the rhizosphere and inside the root cells, where Al complexes are accumulated in the vacuole. Lupins flourish in highly acidic soils where most crops, including other legumes, are unable to grow. Some lupin response mechanisms to Al toxicity are common to other plants, but lupin presents specific tolerance mechanisms, partly as a result of the formation of cluster roots. Al-induced lupin organic acid secretion differs from P-induced secretion, and organic acid transporters functions differ from those in other legumes. Additionally, symbiotic rhizobia can contribute to Al detoxification. After revising the existing knowledge on lupin distinct Al tolerance mechanisms, we conclude that further research is required to elucidate the specific organic acid secretion and Al accumulation mechanisms in this unique legume, but definitely, white lupin arises as a choice crop for cultivation in Al-rich acidic soils in temperate climate regions.

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Active chitinases in the apoplastic fluids of healthy white lupin (Lupinus albus L.) plants

Cited by 7 publications

References 25 publications

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition

Comparative analyses of three legume species reveals conserved and unique root extracellular proteins

Adaptive Mechanisms Make Lupin a Choice Crop for Acidic Soils Affected by Aluminum Toxicity

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