Administration of Lactobacillus paracasei strains improves immunomodulation and changes the composition of gut microbiota leading to improvement of colitis in mice

Kim, Woon-Ki; Jang, Young-Joo; Seo, Boram; Han, Dae Hee; Park, SungJun; Ko, GwangPyo

doi:10.1016/j.jff.2018.11.035

Cited by 36 publications

(33 citation statements)

References 59 publications

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“…L. rhamnosus KBL365 and L. paracasei KBL382 were isolated from the feces of healthy Koreans, as described in a previous study. 46 Briefly, KBL365 and KBL382 strains were cultured in Lactobacilli MRS Agar (BD Difco, Sparks, MD, USA) supplemented with 0.05% L-cysteine-hydrochloride at 37°C under anaerobic conditions maintained with an Anaeropack (Mitsubishi Gas Chemical Company, Inc., Tokyo, Japan) for 24 h. The cells were harvested through centrifugation (1,200 × g) and washed twice with phosphate-buffered saline (PBS) prior to administration to mice. Based on decreases in levels of Th1, Th2, and Th17-type cytokines and an increase in the anti-inflammatory cytokine IL-10 in peripheral blood mononuclear cells in vitro , 46 we selected KBL382 for our AD-like experiment.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

et al. 2020

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Administration of probiotics has been linked to immune regulation and changes in gut microbiota composition, with effects on atopic dermatitis (AD). In this study, we investigated amelioration of the symptoms of AD using Lactobacillus paracasei KBL382 isolated from the feces of healthy Koreans. Mice with Dermatophagoides farinae extract (DFE)-induced AD were fed 1 × 10 9 CFU d −1 of L. paracasei KBL382 for 4 weeks. Oral administration of L. paracasei KBL382 significantly reduced AD-associated skin lesions, epidermal thickening, serum levels of immunoglobulin E, and immune cell infiltration. L. paracasei KBL382-treated mice showed decreased production of T helper (Th)1-, Th2-, and Th17-type cytokines, including thymic stromal lymphopoietin, thymus, and activation-regulated chemokine, and macrophage-derived chemokine, and increased production of the anti-inflammatory cytokine IL-10 and transforming growth factor-β in skin tissue. Intake of L. paracasei KBL382 also increased the proportion of CD4+ CD25+ Foxp3+ regulatory T cells in mesenteric lymph nodes. In addition, administration of L. paracasei KBL382 dramatically changed the composition of gut microbiota in AD mice. Administration of KBL382 significantly ameliorates AD-like symptoms by regulating the immune response and altering the composition of gut microbiota.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

et al. 2020

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“…Previously, we isolated three L. paracasei strains (KBL382, KBL384 and KBL385) from the feces of healthy Koreans and confirmed their strong resistance to bile salts or low pH [ 12 ]. All L. paracasei strains were cultured in anaerobic conditions using Lactobacilli MRS agar (Becton, Dickinson and Company, Sparks, MD, USA) with 0.05% L-cysteine-hydrochloride and Anaeropack (Mitsubishi Gas Chemical Company Inc., Tokyo, Japan) at 37 °C for 24 h as described previously [ 12 ]. Bacteria were collected using centrifugation at 1200× g and washed twice using 1× phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 94%

“…Probiotics such as Lactobacillus spp . have been reported to show strong immunomodulatory effects [ 12 , 13 ]. We previously confirmed the strong anti-inflammatory effects of Lactobacillus paracasei ( L. paracasei ) species, isolated from the feces of healthy Koreans, in the in vivo dextran sodium sulfate (DSS)-induced colitis animal model [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…have been reported to show strong immunomodulatory effects [ 12 , 13 ]. We previously confirmed the strong anti-inflammatory effects of Lactobacillus paracasei ( L. paracasei ) species, isolated from the feces of healthy Koreans, in the in vivo dextran sodium sulfate (DSS)-induced colitis animal model [ 12 ]. In this study, we investigated the modulatory effects of L. paracasei treatment on various enzymes related to glycolysis or macrophage polarization, including HIF1A, iNOS and arginase in stimulated macrophages at the mRNA level in vitro using the rodent bone‐marrow‐derived macrophage (BMDM) model.…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus paracasei treatment modulates mRNA expression in macrophages

Han

Kim

Park

et al. 2020

Biochemistry and Biophysics Reports

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Macrophage metabolic pathways show changes in response to various external stimuli. Especially, increased lipopolysaccharide, an important bacterial component and Toll-like receptor 4 agonist, can induce activity in various macrophage metabolic pathways, including energy production and biosynthesis, as well as high immune responses due to increase in differentiated M1 macrophages. In this study, we confirmed that Lactobacillus paracasei ( L. paracasei ) KBL382, KBL384 and KBL385, isolated from the feces of healthy Koreans, can modulate various enzymes and membrane transporters related to glycolysis or macrophage polarization including hypoxia-inducible factor 1-alpha (HIF1A), inducible nitric oxide synthase (iNOS) and arginase in stimulated macrophages at the mRNA level, using the in vitro rodent bone‐marrow‐derived macrophage (BMDM) model. All L. paracasei exhibited significant down-regulatory effects on mRNAs for glycolysis-related enzymes, including lactate dehydrogenase A, solute carrier family 2 member 1, and triosephosphate isomerase. Moreover, L. paracasei treatment could lead to significant reductions in HIF1A or iNOS mRNA, and induced arginase mRNA in the BMDM model. Therefore, further extensive studies should be performed to support the application of L. paracasei, such as in probiotics or therapeutics, in controlling abnormal immune responses related to macrophage.

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“…22 Lactobacillus paracasei (382 and 385) attenuates colitis in mice by improving the gut microbiota and immunomodulation. 23 We isolated and identified Lactobacillus rhamnosus 2016SWU.05.0601 (Lr-0601), lactic acid bacteria derived from yak yogurt, and found that Lr-0601 showed a high survival rate in a simulated gastrointestinal environment. However, the effects of Lr-0601 on the immune response and gut microbiota remain inconclusive.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus2016SWU.05.0601 regulates immune balance in ovalbumin‐sensitized mice by modulating expression of the immune‐related transcription factors and gut microbiota

Song

et al. 2020

J Sci Food Agric

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BACKGROUND Probiotics regulate host immune balance, which may reduce immune‐related diseases. The effects and mechanisms of Lactobacillus rhamnosus 2016SWU.05.0601 (Lr‐0601) on the immune response in ovalbumin (OVA)‐sensitized mice were explored. RESULTS Lr‐0601 reduced serum immunoglobulin (Ig)E and OVA‐IgE and attenuated the alteration in lung pathology in OVA‐sensitized mice. Lr‐0601 blocked OVA‐induced up‐regulation in serum T helper (Th) 2 and Th17 cytokines but increased the serum levels of Th1 and regulatory T (Treg) cytokines in OVA‐sensitized mice. OVA also markedly reduced the protein levels of spleen T‐box transcription factor and forkhead/winged helix transcription factor p3, leading to the reduced mRNA expression of interferon‐γ and interleukin (IL)‐10. By contrast, OVA markedly increased the protein expression of spleen GATA‐binding protein 3 and retinoid‐related orphan receptor γt, as well as the mRNA expression of spleen IL‐4 and IL‐17. These changes induced by OVA were reversed by Lr‐0601. Moreover, Lr‐0601 helped alleviate OVA‐induced intestinal microbiota dysbiosis. A correlation was found between specific genera and immune‐associated cytokines. CONCLUSION The combined results indicate that Lr‐0601 modulated the balance of Th1/Th2 and Treg/Th17 in OVA‐sensitized mice, which was associated with the regulation of immune‐related transcription factors and gut microbiota. Lr‐0601 can potentially be used as a probiotic for preventing immune‐related diseases. © 2020 Society of Chemical Industry

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Administration of Lactobacillus paracasei strains improves immunomodulation and changes the composition of gut microbiota leading to improvement of colitis in mice

Cited by 36 publications

References 59 publications

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Lactobacillus paracasei treatment modulates mRNA expression in macrophages

Lactobacillus rhamnosus2016SWU.05.0601 regulates immune balance in ovalbumin‐sensitized mice by modulating expression of the immune‐related transcription factors and gut microbiota

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