Adventitious Shoot Formation on Teak (Tectona Grandis L.F.) Callus Cultures Derived From Internodal Segments

Widiyanto, Sri Nanan; Erytrina, Dwi; Rahmania, Heni

doi:10.17660/actahortic.2005.692.20

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…Callus was reported from inter nodal segments in a number of plant species viz., Tectona grandis (Widiyanto et al 2005 ), Mentha arvensis (Johnson et al 2011 ), Dandrocalamus asper (Shroti et al 2012 ), Satureja hortensis (Navroski et al 2012 ), which are in favour of present results.…”

Section: Resultsmentioning

confidence: 41%

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Mittal

Sharma

2017

3 Biotech

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Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of multiple shoot formation was induced when the nodal segments were cultured on MS medium supplemented with BAP (1.0 mg L−1) and 2-iP (0.5 mg L−1). The highest mean number of shoots per nodal explant (7.9 ± 0.45) with highest shoot length (9.3 ± 0.48 cm) and 86% response were achieved on this media and hormonal concentration. The optimum rooting was obtained on ½ strength of MS medium augmented with IBA (0.5 mg L−1) with 8.3 ± 0.46 cm root length and 89% response. Micropropagated plantlets were found to be identical with the mother plant when clonal fidelity of these plantlets were analyzed with inter simple sequence repeat (ISSR) marker. The berberine content was analyzed through LCMS QToF and the highest amount was found in in vitro callus (19.8 µg/gm) followed by stem (9.3 µg/gm) and leaves of field-grown plants (8.4 µg/gm). Further, presence of berberine was confirmed by ESI–MS spectra with protonated molecular ions ([M + H]+) at m/z 336. Furthermore, MS–MS fragmentation pattern confirmed for the presence of berberine in both the samples. Both the spectra (standard and samples) showed common peaks for berberine in the form of protonated molecular ions ([M + H]+) at m/z 320, m/z 304, m/z 292, m/z 278 in MS/MS mode. The study revealed that developed protocol is potent for rapid mass propagation of this plant species with high accumulation of important secondary metabolite berberine.

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Section: Resultsmentioning

confidence: 41%

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Mittal

Sharma

2017

3 Biotech

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“…In vitro propagation has been successfully applied in Tectona grandis and it has become an alternative tool to overcome some problems occurring in sexual regeneration (Widiyanto et al 2005). Regenerative organs such as pre-existing shoots, meristem shoot-tips, nodal segments or seedling organs have been widely used as explants (Gupta et al 1980, Mascarenhas et al 1987, Apavatjrut et al 1988, Devi et al 1994.…”

Section: Discussionmentioning

confidence: 99%

“…However, no report of the successful genetic transformation of teak has been published to date (Widiyanto et al 2009). In attempts to develop the transformation procedure of teak, many researchers have studied the regeneration ability of teak callus tissues to develop shoots and found that the frequency of adventitious shoot formation from teak callus was either very low or absent (Widiyanto et al 2003, Widiyanto et al 2005. Therefore, in the present study, two experiments were conducted.…”

Section: Introductionmentioning

confidence: 91%

“…Internode and hypocotyls explants of teak proved to be the most responsive explant type among the four explants tried. Widiyanto et al (2005) also reported shoot organogenesis in teak on calli induced from internodal segments on Woody Plant Medium containing 1.0 μM thidiazuron (TDZ) in combination with 0.01 μM indole butyric acid (IBA).…”

Section: Shoot Formationmentioning

confidence: 99%

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Effect of various plant growth regulators on in vitro seed germination and shoot organogenesis in Tectona grandis L.f.

Mishra¹,

Bhadrawale²,

Yadav³

et al. 2018

Trop. Plant Res.

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In the present study efforts were made to enhance in vitro seed germination and achieve shoot organogenesis through different explants of Tectona grandis, a hardwood timber species known throughout the world for its high-value wood. Seed germination was significantly affected by seed inoculation on different strengths of MS medium and different GA 3 concentrations. Different strengths of MS media combined with different GA 3 concentration significantly affect seed germination in vitro. Maximum seed germination (96%) was obtained on 0.4% GA 3 combined with half strength MS medium. The effect of types of explants (leaf, internode, hypocotyl and root of seedlings), different concentrations of thidiazuron (TDZ) (0.1, 1.0 and 10 μM) and auxins (IBA, IAA and NAA) on callus induction and shoot formation was investigated. After 30 days of inoculation, it was observed that explant type had a significant effect on callus formation. Maximum callus was obtained on internode explants (56.17%) which were statistically on par with callus obtained on leaf explants (54.32%). The effect of TDZ on callus and shoot formation was also observed. TDZ with higher concentration (1 μM) gave maximum callus (48.6%). Whereas lower concentration of TDZ (0.1 μM) was effective for shoot formation from both internode and hypocotyl explants. IAA and NAA were also helpful in shoot formation whereas IBA retards shoot formation. For shoot organogenesis internode was found to be most responsive explant type followed by hypocotyls and leaf. Roots were found to be the least responsive explant.

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“…1g, h with the maximum number of shoots (33.2 ± 0.41) and the average length of the shoot was 6.36 ± 1.66 cm Table 3. Proliferative features of cytokinins TDZ and BAP play various roles in plant development during the formation and activity of shoot meristems [20][21][22] .…”

Section: Methodsmentioning

confidence: 99%

Untitled

2019

IJPSR

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The present study was developed a suitable protocol for callus induction and plant regeneration of Cleome gynandra L. from leaf explants. Callus was initiated from leaf explants on Murashige and Skoog (MS) medium supplemented with different concentration and combinations of plant growth regulators. They are using (5-25 µM) of BAP (6-benzyladenine), KN (kinetin), and TDZ (thidiazuron) cytokinins along with (2-10 µM) different auxins [NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid)]. Maximum regenerative callus (95%) was observed on TDZ + NAA (10 µM + 2 µM). Organogenic calli were transferred to shoot induction medium containing a different concentration of cytokinins (BAP, KIN, TDZ) ranging from 5-25 µM. Among the cytokinins tested BAP 10 µM produced number of shoots (35.2) with maximum shoot induction frequency (98%). Isolated shoots were carefully transferred to half-strength MS medium supplemented with auxins (IAA, IBA, and NAA) ranging from 2-10 µM. The maximum numbers of roots (8.8) were observed on 4 µM IBA. The rooted plantlets were successfully acclimatization.

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Adventitious Shoot Formation on Teak (Tectona Grandis L.F.) Callus Cultures Derived From Internodal Segments

Cited by 8 publications

References 7 publications

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Enhanced production of berberine in In vitro regenerated cell of Tinospora cordifolia and its analysis through LCMS QToF

Effect of various plant growth regulators on in vitro seed germination and shoot organogenesis in Tectona grandis L.f.

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