Aging effects on the diurnal patterns of gut microbial composition in male and female mice

Abstract

Composition of the gut microbiota changes with aging and plays an important role in age-associated disease such as metabolic syndrome, cancer, and neurodegeneration. The gut microbiota composition oscillates through the day, and the disruption of their diurnal rhythm results in gut dysbiosis leading to metabolic and immune dysfunctions. It is well documented that circadian rhythm changes with age in several biological functions such as sleep, body temperature, and hormone secretion. However, it is not defined whether the diurnal pattern of gut microbial composition is affected by aging. To evaluate aging effects on the diurnal pattern of the gut microbiome, we evaluated the taxa profiles of cecal contents obtained from young and aged mice of both sexes at daytime and nighttime points by 16S rRNA gene sequencing. At the phylum level, the ratio of Firmicutes to Bacteroidetes and the relative abundances of Verrucomicrobia and Cyanobacteria were increased in aged male mice at night compared with that of young male mice. Meanwhile, the relative abundances of Sutterellaceae, Alloprevotella, Lachnospiraceae UCG-001, and Parasutterella increased in aged female mice at night compared with that of young female mice. The Lachnospiraceae NK4A136 group relative abundance increased in aged mice of both sexes but at opposite time points. These results showed the changes in diurnal patterns of gut microbial composition with aging, which varied depending on the sex of the host. We suggest that disturbed diurnal patterns of the gut microbiome can be a factor for the underlying mechanism of age-associated gut dysbiosis.

Sacha inchi albumin delays skin‐aging by alleviating inflammation, oxidative stress and regulating gut microbiota ind‐galactoseinduced‐aging mice

“…It was found in this study that treatment with SIA significantly reduced the relative abundance of Helicobacter and Alloprevotella , which is dominant in some age‐induced diseases 60 . As previous report that Alloprevotella was not detected in young female mice but was present in high levels in aged female mice 61 . Alistipes , Ligilactobacillus , and Lachnospiracae_NK4A136_ groups are also associated with inflammation, and the latter is negatively associated with inflammation 62,63 .…”

“…60 As previous report that Alloprevotella was not detected in young female mice but was present in high levels in aged female mice. 61 Alistipes, Ligilactobacillus, and Lachnospira-cae_NK4A136_ groups are also associated with inflammation, and the latter is negatively associated with inflammation. 62,63 In the present study, the relative abundance of Lachnospira-cae_NK4A136_ groups was significantly reduced and that of the Alistipes and Ligilactobacillus was significantly changed after SIA intervention, which indicated that SIA might delay skin aging by alleviating inflammation.…”

Gut microbiota-based pharmacokinetic-pharmacodynamic study and molecular mechanism of specnuezhenide in the treatment of colorectal cancer targeting carboxylesterase

“…8 E demonstrates the representative genera with significant changes between tumor group and normal group. Lachnospiraceae UCG- 001 has been reported to be more abundant in older female mice than in younger females, suggesting that it is associated with aging [ 43 ]. Prevotellaceae_UCG-001 is associated with the pathogenesis of colitis [ 44 ].…”