Agronomic characterization of diploid hybrid families derived from crosses between haploids of the common potato and three wild Argentinian tuber-bearing species

Santini, Matías; Camadro, Elsa Lucila; Marcellán, Olga; Erazzú, Luis Ernesto

doi:10.1007/bf02855788

Cited by 28 publications

(21 citation statements)

References 24 publications

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“…Santini et al (1976), working with grl 9 tbr hybrids, detected desirable tuber characteristics like high yield and tuber quality. More recently, Santini et al (2000) reported that hybrid families derived from crosses between tbr haploids and pooled pollen from spg, grl and Solanum chacoense (chc, 2n = 2x = 24, 2EBN) showed a positive influence of the wild species on desirable tuber traits such as dry matter content (21.8 vs. 17-20% in tbr reported by Cacace et al 1994), tuber number, size and shape, skin texture and colour, and eye depth.…”

Section: Introductionmentioning

confidence: 94%

“…These species have numerous characteristics of agronomic interest such as resistance/tolerance to water and temperature stresses, nematodes, harmful insects, fungi, bacteria and viruses, as well as desirable culinary and industrial qualities like high dry matter content, low reducing sugar content and vitamin C Santini et al 2000;Davies et al 2002;Oltmans and Novy 2002;Jansky and Peloquin 2005). Ross (1986) emphasized the importance of wild tuber-bearing Solanum species in the concrete contribution of genes of interest to potato breeding.…”

Section: Introductionmentioning

confidence: 99%

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Persistence over time, overlapping distribution and molecular indications of interspecific hybridization in wild potato populations of Northwest Argentina

2009

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Solanum gourlayi and Solanum spegazzinii, wild potatoes endemic to Argentina, possess desirable traits for breeding. In periodical regenerations of accessions, variability was detected for morphology and breeding barriers. The persistence of these populations in nature was evaluated after more than 20 years. Both species were observed in all visited sites, along with other wild and cultivated potatoes. Chromosome numbers coincided with the originally reported, except for one population of Solanum gourlayi, with diploid and tetraploid cytotypes. The accompanying flora and environmental conditions revealed important alterations as the result of road construction, excessive stocking rates and overgrazing. Principal coordinate and cluster analyses and an AMOVA using AFLP data of three original accessions and the corresponding new accessions revealed high molecular variability and extensive overlapping. Plant grouping of accessions occurred at a distance of 0.58 for S. gourlayi, 0.62 for S. spegazzinii and 0.67 for both species. The role of natural hybridization and sexual polyploidization in the evolution of sympatric populations of wild potatoes is discussed.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Persistence over time, overlapping distribution and molecular indications of interspecific hybridization in wild potato populations of Northwest Argentina

2009

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“…In the past, several authors observed male sterility in F 1 hybrids derived from matings involving various wild and cultivated species. In particular, male sterility has been reported to be a result of interactions between S. tuberosum cytoplasm and nuclear genetic factors in the wild species S. sanctae-rosae, S. infundibuliforme, S. raphanifolium, S. curtilobum, S. acaule, S. commersonii, S. chacoense, S. spegazzini, and S. stoloniferum (Lamm 1941(Lamm , 1953Brown 1984;Hermunstad and Peloquin 1985;Tucci et al 1996;Santini et al 2000;Carputo et al 2003b) and in other cultivated potatoes (Ross et al 1964;Grun 1973;Hanneman and Peloquin 1981), in S. verrucosum cytoplasm and nuclear genes of S. phureja, S. chacoense, and S. tuberosum (Buck 1960;Abdalla and Hermsen 1972), and in S. demissum cytoplasm and nuclear genes of S. tuberosum (Dionne 1961).…”

Section: Nuclear-cytoplasmic Male Sterility Barriersmentioning

confidence: 99%

Substitutes for genome differentiation in tuber-bearing Solanum: interspecific pollen-pistil incompatibility, nuclear-cytoplasmic male sterility, and endosperm

2004

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The cultivated potato, Solanum tuberosum L. (2n=4x=48), has a very large number of related wild and cultivated tuber-bearing species widely distributed in the Americas. These species, grouped in 16 taxonomic series, range from the diploid to the hexaploid level. Polyploid species are either disomic or polysomic, and sexual polyploidization via genetically controlled 2 n gametes has played a major role in their evolution. Species are separated in nature by geographical and ecological barriers. However, there are several examples of sympatric species that share the same niches but do not readily cross (i.e., the diploids S. commersonii and S. chacoense in certain areas of Argentina). External barriers alone are, therefore, not sufficient to explain species integrity. In addition, there is no strong evidence indicating that genome differentiation is important in the group. In this review we present evidence supporting the assertion that interspecific pollen-pistil incompatibility, nuclear-cytoplasmic male sterility, and the endosperm are major forces that strengthen the external hybridization barriers allowing, at the same time and under specific circumstances, a certain amount of gene exchange without jeopardizing the integrity of the species.

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“…In a previous work, Santini et al (2000) reported the great potential of the diploid hybrid families used in this study for improvement of traits such as tuber number and shape and dry matter content. Notwithstanding, they found that all plants had ''tetrad''-type pollen sterility, probably determined by gene-cytoplasm interactions.…”

Section: Discussionmentioning

confidence: 97%

“…In these families, Santini et al (2000) detected great variability and significant statistically differences among them for important agronomic characters, including high tuber yield and dry matter content. They could not be used in recurrent selection programs or as male progenitors en 4x · 2x crosses for they were completely male sterile, exhibiting a ''tetrad''-type sterility.…”

Section: Introductionmentioning

confidence: 97%

Direct and indirect detection of 2n eggs in hybrid diploid families derived from haploid tbr × wild species crosses

Erazzú

Camadro

2006

Euphytica

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The analytic-synthetic scheme makes use of haploidization and 2n gametes-mediated sexual polyploidization to incorporate genetic diversity from wild related species in potato breeding. Whereas 2n pollen can be directly screened by microscopy, 2n eggs are routinely screened indirectly by interploid crosses, a timeconsuming and inaccurate technique. In five haploid tbr · wild diploid species families with variability for agronomic characters, 2n eggs were screened indirectly to incorporate this germplasm into a useful form and directly by microscopy to establish the feasibility of this approach. For the indirect screening, at least 15 flowers of each of 49 genotypes/family were crossed with a commercial cultivar. Eighty-five fruits were produced; only 15 of them had seeds (1-25). Chromosome numbers were determined in a random sample of five seeds/ fruit, resulting diploid in two genotypic combinations and tetraploid in another two. For the direct screening, eight combinations of staining and destaining (times and concentrations) were evaluated in detached ovules to adjust a clarification technique. The best combination was one day of staining and one day of destaining in 0.5 % acetic acid. In an indirectly detected diplogynoid, the average diameters of the central cell nuclei (CCN) and nucleoli (CCNu) were, respectively, 9.3 l and 3.4 l. Gametophytes with diametres 1.26 times the average were considered 2n. By crosses, eleven seeds were obtained in one fruit of this clone whereas the 2n egg frequency determined by microscopy was 7%. The direct technique is easy to carry out once the eye is trained and its results better reflect actual 2n gamete frequencies.

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Agronomic characterization of diploid hybrid families derived from crosses between haploids of the common potato and three wild Argentinian tuber-bearing species

Cited by 28 publications

References 24 publications

Persistence over time, overlapping distribution and molecular indications of interspecific hybridization in wild potato populations of Northwest Argentina

Persistence over time, overlapping distribution and molecular indications of interspecific hybridization in wild potato populations of Northwest Argentina

Substitutes for genome differentiation in tuber-bearing Solanum: interspecific pollen-pistil incompatibility, nuclear-cytoplasmic male sterility, and endosperm

Direct and indirect detection of 2n eggs in hybrid diploid families derived from haploid tbr × wild species crosses

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