Alginate and Nanocellulose Dressings With Extract From Salmon Roe Reduce Inflammation and Accelerate Healing of Porcine Burn Wounds

Abstract

Partial thickness thermal burn wounds are characterized by prolonged inflammatory response, oxidative stress, tissue damage, and secondary necrosis. An optimal dressing for burn wounds would reduce inflammation and oxidative stress while providing a moist, absorbent, and protective cover. We have developed an extract from unfertilized salmon roe containing components with potential anti-inflammatory and antioxidative properties, called HTX. HTX has been combined with alginate from brown algae and nanocellulose from tunicates, and 3D printed into a solid hydrogel wound dressing called Collex. Here, Collex was tested on partial thickness burn wounds in Göttingen minipigs compared to Jelonet, and a variant of Collex without HTX. We found that dermal treatment of burn wounds with Collex resulted in accelerated healing at a majority of measured points over 23 days, compared to treatment with Jelonet. In comparison to Collex without HTX, Collex enhanced healing in the first week after trauma where wound progression was pronounced. Notably, Collex reduced the inflammatory response in the early post-injury phase. The anti-inflammatory response of Collex was investigated in more detail on activated M1 macrophages. We found that Collex, as well as HTX alone, significantly reduced secretion of pro-inflammatory interleukin-1β as well as intracellular levels of oxidative stress. The results from this study indicate that Collex is a potent dressing for treatment of burn wounds, with the anti-inflammatory effect of HTX beneficial in the initial phase, and the moist qualities of the hydrogel favorable both in the initial and the proceeding proliferative phase of wound healing.

Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds

“…The extract of Collex was found non-cytotoxic when evaluated according to ISO 10993-5:2009. Results from a minipig trial [ 37 ] suggest that Collex has significant potential to improve healing of burn wounds and motivates further clinical testing of Collex in the healing of burns and chronic hard-to-heal wounds.…”

“…This work combines renewable, all-marine-sourced products to create a 3D printed wound dressing, referred to as Collex™, developed for the healing of hard-to-heal wounds, such as chronic wounds and burns, which are both low on exudate. The product developed herein was evaluated in parallel on porcine burn wounds, which indicated that Collex is a potent dressing for the treatment of burn wounds [ 37 ]. The work presented herein describes the ink optimization process and characterization of the material properties of this new wound healing dressing, which includes in vitro assays to determine cytotoxicity, the release profile of HTX, and its effect on human fibroblasts.…”

Intelligent Electrospinning Nanofibrous Membranes for Monitoring and Promotion of Wound Healing

“…Subsequently, the defined inflammatory phase begins 24–48 h after the wound formation [ 37 , 40 ]. During this period, inflammatory mediators and chemokines related to the assembly and differentiation of leukocyte, lymphocyte and monocyte would increase sharply [ 41 , 42 ]. With the enrichment of these cells and chemokines, the proliferation of fibroblasts and endothelial cells begins in earnest.…”

Evaluation of inherent properties of the carboxymethyl cellulose (CMC) for potential application in tissue engineering focusing on bone regeneration

“…Nanocellulose-based composite dressings for burn wound healing. [200][201][202] Drug delivery systems 1. Nanocellulose-based carrier system for delivering anticancer drugs.…”

“…Nanocellulose‐based hydrogel dressings for chronic wound healing 198,199 Nanocellulose‐based composite dressings for burn wound healing 200–202 …”