Amino acid residues involved in the interaction with the intrinsic agonist (R)-octopamine in the .BETA.-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Chen, Xi; Ohta, Hiroto; Sasaki, Kensuke; Ozoe, Fumiyo; Ozoe, Yoshihisa

doi:10.1584/jpestics.g11-48

Cited by 23 publications

(27 citation statements)

References 30 publications

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“…These residues play an important role in ligand binding. It was reported that Asp residue in TM3 was a key residue interacting with the β‐OH group of octopamine in the binding pocket of octopamine receptors …”

Section: Resultsmentioning

confidence: 99%

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Huang

et al. 2019

Pest Management Science

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BACKGROUND Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, regulates and modulates many physiological and behavioral processes in insects. It mediates its effects by binding to specific octopamine receptors, which belong to the superfamily of G‐protein coupled receptors (GPCRs). The expression profiles of octopamine receptor genes have been well documented in different developmental stages and multiple tissue types in several different insect orders. However, little work has addressed this issue in Hemiptera. RESULTS In this study, we cloned four octopamine receptor genes from brown planthopper. The deduced amino acid sequences share high identity with other insect homologues and have the characteristic GPCRs domain architecture: seven transmembrane domains. These genes were expressed in all developmental stages and examined tissues. The expression of NlOA2B3 and NlOA3 was relatively higher in egg and first instar nymph stage than in other stages and other receptor genes. All of these receptor genes were more highly expressed in brain than other tissues. CONCLUSION The identification of octopamine receptor genes in this study will provide a foundation for investigating the diverse roles played by NlOARs and for exploring specific target sites for chemicals that control agricultural pests. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Huang

et al. 2019

Pest Management Science

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“…In previous studies, we cloned cDNAs encoding two OARs (BmOAR1 and BmOAR2) and two tyramine receptors (TARs) (BmTAR1 and BmTAR2) from B. mori . We functionally and pharmacologically characterised these GPCRs . When they were expressed in HEK‐293 cells, BmOAR1, BmOAR2, BmTAR1 and BmTAR2 were found to be most likely coupled to the G q /G s , G s , G i and G q proteins respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Activation of β ‐AL OARs results in the selective elevation of the intracellular cAMP levels. We have cloned cDNAs encoding α ‐ and β ‐AL OARs from silkworms ( Bombyx mori ), BmOAR1 and BmOAR2 respectively, and studied the signalling and pharmacological properties of the receptors that are stably expressed in HEK‐293 cells . Given the multiple types of phenolamine receptor (PAR), identifying receptors that are specific to amitraz is important.…”

Section: Introductionmentioning

confidence: 99%

Amitraz and its metabolite differentially activate α‐ and β‐adrenergic‐like octopamine receptors

Kita

Hayashi

Ohtani

et al. 2016

Pest Management Science

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“…, ; Chen et al . ). Interaction of the p‐OH group of both biogenic amines with one or even two serine residues in TM5 (Ser 5.42/5.46 ) has also been shown (Chatwin et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Chen et al . ). In contrast to these amino acids, unequivocal experimental evidence for the binding partner of the β‐OH group of octopamine is missing.…”

Section: Discussionmentioning

confidence: 97%

Molecular, pharmacological, and signaling properties of octopamine receptors from honeybee (Apis mellifera) brain

Balfanz

Jordan

Langenstück

et al. 2013

Journal of Neurochemistry

102

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in the nanomolar range. Receptor activity was most efficiently blocked by mianserin, a substance with antidepressant activity in vertebrates. The rank order of inhibitory potency for potential receptor antagonists was very similar on all four honeybee receptors with mianserin >> cyproheptadine > metoclopramide > chlorpromazine > phentolamine. The subroot of octopamine receptors activating adenylyl cyclases is the largest that has so far been characterized in arthropods, and it should now be possible to unravel the contribution of individual receptors to the physiology and behavior of honeybees.

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Amino acid residues involved in the interaction with the intrinsic agonist (R)-octopamine in the .BETA.-adrenergic-like octopamine receptor from the silkworm Bombyx mori

Cited by 23 publications

References 30 publications

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Amitraz and its metabolite differentially activate α‐ and β‐adrenergic‐like octopamine receptors

Molecular, pharmacological, and signaling properties of octopamine receptors from honeybee (Apis mellifera) brain

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