Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis

Lee, Soon Dong

doi:10.1099/ijs.0.006577-0

Cited by 43 publications

(25 citation statements)

References 26 publications

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“…The major menaquinone found was MK-9(H 4 ) (85 %); followed by MK-7(H 4 ) (5 %), MK-8(H 4 ) (3 %), MK-10(H 2 ) (3 %) and MK-10(H 4 ) (3 %). These data are in line with the classification of the isolate in the genus Amycolatopsis (Lechevalier et al, 1986;Lee, 2009). The major cellular fatty acids are iso C 16 : 0 2-OH (19.3 %) and iso-C 16 : 0 (18.6 %) (Table S1).…”

Section: Introductionsupporting

confidence: 88%

Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine

Tatar

Sazak

Güven

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel halotolerant actinomycete, designated strain BNT52T , was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus Amycolatopsis and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52 T was most closely related to Amycolatopsis nigrescens CSC17Ta-90 T (96.7 %), strains of species of the genus Amycolatopsis were lower than 96.2 %. The isolate grew at 20-37 6C, pH 6-12 and in the presence of 0-10 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C 16 : 0 2-OH and iso-C 16 : 0 . The predominant menaquinone was MK-9(H 4 ). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52 T represents a novel species within the genus Amycolatopsis for which the name Amycolatopsis cihanbeyliensis sp. nov. is proposed (type strain BNT52 IntroductionThe genus Amycolatopsis, first established by Lechevalier et al. (1986), belongs to the family Pseudonocardiaceae and is a member of the suborder Pseudonocardineae (Embley et al., 1988;Warwick et al., 1994;Stackebrandt et al., 1997). The members of the genus are Gram-reaction-positive, nonacid-fast, non-motile actinomycetes that form branched vegetative hyphae that undergo fragmentation into rod-like and squarish elements. The genus contains halophilic, alkaliphilic, mesophilic, thermophilic and pathogenic species (SaintpierreBonaccio et al., 2005;Lee, 2006;Lee et al., 2006;Groth et al., 2007). The species of the genus Amycolatopsis have mesodiaminopimelic acid, arabinose and galactose in the wall peptidoglycan (wall chemotype IV sensu Lechevalier & Lechevalier, 1970), fatty acids rich in iso-and anteiso-branched components and a lack of mycolic acids (Takahashi, 2001). The predominant menaquinone type is MK-9(H4); phosphatidylethanolamine is a diagnostic phospholipid (type II sensu Lechevalier et al., 1977) and the G+C content of genomic DNA ranges from 66 to 73 mol%. The aim of the present polyphasic study was to clarify the taxonomic position of the novel strain BNT52 T of the genus Amycolatopsis, isolated from a soil sample, collected from Cihanbeyli Salt Mine, in Turkey.During research on biodiversity of culturable actinomycetes from a soil sample collected from Cihanbeyli Salt Mine (GPS coordinates for the sampling site are 38 u 459 500 N 33 u 099 260 E), located in the central Anatolia region, actinomycete strain BNT52T was isolated on modified Bennett's agar (Jones, 1949) supplemented with 5 % NaCl (w/v), which had been inoculated with a soil suspension and incubated at 28 u C for 21 days. The organism was maintained on modified Bennett's agar slopes containing ...

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Section: Introductionsupporting

confidence: 88%

Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine

Tatar

Sazak

Güven

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…The genus was proposed by Lechevalier et al (1986) and its description has been emended recently by Lee (2009). Many novel species of this genus have been isolated from diverse environments such as clinical material (Labeda et al, 2003;Huang et al, 2004), natural caves (Lee, 2006), ocean sediment (Bian et al, 2009), soil (Tan et al, 2006;Tseng et al, 2006;Lee, 2009) and vegetable matter (Goodfellow et al, 2001). The present study was designed to determine the status of Amycolatopsis strain RM287…”

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confidence: 99%

Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr.

Duangmal

Mingma

Pathom-aree

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr. Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanA novel actinomycete, designated strain RM287 T , was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain RM287 properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H 4 ). The major fatty acids were iso-C 16 : 0 iso-C 15 : 0 , iso 2-OH-C 16 : 0 and iso-C 17 : 0 . The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287 T represented a novel species of the genus Amycolatopsis. The proposed name for this organism is Amycolatopsis samaneae sp. nov., with the type strain RM287 T (5TISTR 1919The genus Amycolatopsis comprises a large group of commercially and medically important actinomycetes. ). The present study was designed to determine the status of Amycolatopsis strain RM287 T , isolated from surface-sterilized plant roots, using a polyphasic taxonomic approach. It was evident from the data obtained that this organism represents a novel species of the genus Amycolatopsis.As part of our discovery programme for endophytic actinomycetes, strain RM287T was isolated from a leguminous plant using starch casein agar (Küster & Williams, 1964) supplemented with ketokonazole (100 mg ml 21 ) and nalidixic acid (25 mg ml 21 ). Plates that had been inoculated with surface-sterilized crushed root suspensions of Samanea saman were incubated at 28 u C for 14 days. The Abbreviation: ISP, International Streptomyces Project.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RM287T is GQ381310.

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“…The genus encompasses alkaliphilic, halophilic, mesophilic and thermophilic species that can be distinguished using a combination of phenotypic properties (Goodfellow et al, 2001;Labeda et al, 2003;Tan et al, 2007). These taxa fall into several multi-and singlemembered subclades in the Amycolatopsis 16S rRNA gene tree (Tseng et al, 2006;Bian et al, 2009;Lee, 2009). The thermophilic species form a distinct phyletic line, the Amycolatopsis methanolica subclade, which also encompasses Amycolatopsis eurytherma, Amycolatopsis thermoflava and Amycolatopsis tucumanensis (Chun et al, 1999;Kim et al, 2002;Albarracín et al, 2010).…”

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confidence: 99%

Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., thermophilic actinomycetes isolated from arid soil

Zucchi

Tan

Goodfellow

2012

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic positions of two thermophilic actinomycetes isolated from an arid Australian soil sample were established based on an investigation using a polyphasic taxonomic approach. The organisms had chemical and morphological properties typical of members of the genus Amycolatopsis and formed distinct phyletic lines in the Amycolatopsis methanolica 16S rRNA subclade. The two organisms were distinguished from one another and from the type strains of related species of the genus Amycolatopsis using a range of phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the two isolates be classified in the genus Amycolatopsis as Amycolatopsis thermophila sp. nov. (type strain GY088T = NCIMB 14699T = NRRL B-24836T) and Amycolatopsis viridis sp. nov. (type strain GY115T = NCIMB 14700T = NRRL B-24837T).

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Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis

Cited by 43 publications

References 26 publications

Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine

Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine

Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr.

Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., thermophilic actinomycetes isolated from arid soil

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