An application of high-speed counter-current chromatography coupled with electrospray ionization mass spectrometry for separation and online identification of coumarins from Peucedanum praeruptorum Dunn

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

“…The 15 known compounds were identified as (+)-praeruptorin A (2) [20,21], (+)-praeruptorin B (3) [20,21], (+)-praeruptorin E (4) [20][21][22], selinidin (5) [23], cis-3′,4′-diisovalerylkhellactone (6) [19,24], pteryxin (7) [6], suksdorfin (8) [25,26], Pd-Ib (9) [27,28], qianhucoumarin D (10) [20,21], (+)-samidin (11) [25,[29][30][31], laserpitin (12) [32], (9R,10R)-9-acetoxy-8,8-dimethyl-9,10-dihydro-2H,8H-benzo [1,2-b:3,4-b′]dipyran-2-one-10-yl-ester (13) [33], bergapten (14) [34,35], xanthotoxin (15) [36], and falcalindiol (16) [37][38][39][40].…”

Two-dimensional countercurrent chromatography × high performance liquid chromatography with heart-cutting and stop-and-go techniques for preparative isolation of coumarin derivatives from Peucedanum praeruptorum Dunn

“…Similar report by coupling CCC and prep-HPLC in off-line mode has also successfully seperated d-laserpitin from P. praeruptorum [20]. In addition, combination of CCC with ESI-MS resulted in the isolation of seven coumarins from P. praeruptorum [21]. The light petroleum-ethyl acetate-methanol-water solvent system was applied in all of these CCC separations.…”

“…The solvent system composed of light petroleum/ethyl acetate/methanol/water used for the CCC separation of P. praeruptorum was previously reported [6,[19][20][21]. However, the selectivity and resolution of CCC separation may be improved, if the solvent system was further comprehensively evaluated by considering the Kd values, stationary phase fraction (Sf), as well as the intermolecular properties of the solvents.…”

“…The chemical structures of those isolated compounds with purity higher than 90% were identified as praeroside III (1) [25], umbelliferone (2) [26], rutaretin (3) [27], (−)-cis-khellactone (4) [27], nodakenetin (5) [19,28], bergapten (6) [28,29], xanthotoxin (7) [29], Pd-Ib (8) [21], d-laserpitin (9) [20], nodakenetin tiglate (10) [30], praeruptorin A (11) [21], decursinol angelate (12) [28], corymbocoumarin (13) [31], qianhucoumarin J (14) [20], praeruptorin B (15) [21] and praeruptorin E (16) [21], by ESI-MS and 1 H, 13 C NMR data (Supporting Information), which were consistent with the authentic samples or literature data.…”

Semi‐preparative high‐speed counter‐current chromatography separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn by pH‐gradient elution

“…Hence, it has been widely used in separation of active components from traditional Chinese herbs and other natural products in recent years. Many successful applications of HSCCC have been reported for purification of alkaloids [5], flavonoids [6], coumarins [7], anthraquinones [8], organic acids [9], diterpenoids [10], saponins [11], and so on.…”