An automated Teflon microfluidic peptide synthesizer

Zheng, Huan; Wang, Weizhi; Li, Xiaojun; Wang, Zihua; Hood, Leroy; Lausted, Christopher; Hu, Zhiyuan

doi:10.1039/c3lc50632k

Cited by 26 publications

(21 citation statements)

References 24 publications

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“…It is low cost ($V50 per kg), optically transparent, and crucially its relatively low melting temperature for a uoropolymer (165 C) means it can be easily melt-processed using a standard laboratory oven with no need for the expensive hydraulic presses that feature in previous reports describing monolithic Teon chips. 8,9,22 Microstructures were fabricated in Dyneon THV using a replica moulding strategy as illustrated in Fig. 1.…”

Section: Chip Fabricationmentioning

confidence: 99%

“…325 C for PTFE, 315 C for PFA), that mean expensive specialised equipment is required for moulding procedures. 8,9 Consequently researchers oen opt to assemble uidic manifolds from off-the-shelf uoropolymer capillary tubing 6,7,10 as a more user-friendly option for long-term experimentation. In such systems uidic architectures cannot be arbitrarily designed however, meaning complex structures such as the extended path length ow cells needed for sensitive absorbance measurement are unobtainable.…”

Section: Introductionmentioning

confidence: 99%

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Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

et al. 2020

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Section: Chip Fabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

et al. 2020

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“…Combinatorial prototyping strategies have also allowed preparation of large libraries in single batch reactions [10]. Improvements to synthesizers, including continuous-flow and microwave-assisted techniques, have allowed ultra-rapid fabrication for high-throughput prototyping synthesis [11][12][13][14][15][16]. While these new tools have proven effective, they require expensive equipment or specialized fabrication tools.…”

Section: Introductionmentioning

confidence: 99%

Design and Validation of a Frugal, Automated, Solid-Phase Peptide Synthesizer

Kallmyer

Rider

Reuel

2020

Preprint

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Solid phase peptide synthesis (SPPS) has enabled widespread use of synthetic peptides in applications ranging from pharmaceuticals to materials science. The demand for synthetic peptides has driven recent efforts to produce automated SPPS synthesizers which utilize fluidhandling components common to chemistry laboratories to drive costs down to several thousand dollars. Herein, we describe the design and validation of a more 'frugal' SPPS synthesizer that uses inexpensive, consumer-grade fluid-handling components to achieve a prototype price point between US$300 and $600. We demonstrated functionality by preparing and characterizing peptides with a variety of distinct properties including binding functionality, nanoscale selfassembly, and oxidation-induced fluorescence. This system yielded micromoles of peptide at a cost of approximately $1/residue, a cost which may be further reduced by optimization and bulk purchasing.

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“…OBOC library screening 19 - 21 is efficient and has been applied in discovery of novel peptides targeting at cancer and other diseases. In addition, OBOC library combining in situ mass spectrometry (MS) sequencing and identification by using a microfluidic chip 22 - 24 with magnetic trapping and sheath flow sorting functions have been successfully employed in sorting a large number of peptide beads library in our previous works 25 . However, the screening efficiency of this method can be further improved by applying computational simulation in designing the library to limit mutations of residues in peptide for favorite interactions rather than aimless random mutations.…”

Section: Introductionmentioning

confidence: 99%

HER2 Targeting Peptides Screening and Applications in Tumor Imaging and Drug Delivery

et al. 2016

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Herein, computational-aided one-bead-one-compound (OBOC) peptide library design combined with in situ single-bead sequencing microarray methods were successfully applied in screening peptides targeting at human epidermal growth factor receptor-2 (HER2), a biomarker of human breast cancer. As a result, 72 novel peptides clustered into three sequence motifs which are PYL***NP, YYL***NP and PPL***NP were acquired. Particularly one of the peptides, P51, has nanomolar affinity and high specificity for HER2 in ex vivo and in vivo tests. Moreover, doxorubicin (DOX)-loaded liposome nanoparticles were modified with peptide P51 or P25 and demonstrated to improve the targeted delivery against HER2 positive cells. Our study provides an efficient peptide screening method with a combination of techniques and the novel screened peptides with a clear binding site on HER2 can be used as probes for tumor imaging and targeted drug delivery.

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An automated Teflon microfluidic peptide synthesizer

Cited by 26 publications

References 24 publications

Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics

Design and Validation of a Frugal, Automated, Solid-Phase Peptide Synthesizer

HER2 Targeting Peptides Screening and Applications in Tumor Imaging and Drug Delivery

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