An electrochemical biosensor based on multi-wall carbon nanotube–modified screen-printed electrode immobilized by uricase for the detection of salivary uric acid

Shi, Weishan; Li, Jing; Wu, Jie; Wei, Qianying; Chen, Cuili; Bao, Ning; Yu, Chunmei; Gu, Hongchen

doi:10.1007/s00216-020-02860-w

Cited by 55 publications

(33 citation statements)

References 39 publications

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“…Drop-casting of CNTs onto electrode surfaces, followed by drop-casting of the biorecognition element, has been commonplace throughout research in this field. A good example of this has been recently published by Shi et al who fabricated a screen-printed electrode (SPE)-based sensor for uric acid through drop-casting of MWCNT followed by uricase onto the carbon working electrode [18]. The MWCNT were chosen for this platform due to their excellent ability to facilitate electron transfer between the analyte and electrode, in addition to the significantly enhanced surface area for uricase binding.…”

Section: Carbon Nanotube-based Biosensorsmentioning

confidence: 99%

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Crapnell

Banks

2021

Microchim Acta

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Research into electrochemical biosensors represents a significant portion of the large interdisciplinary field of biosensing. The drive to develop reliable, sensitive, and selective biosensing platforms for key environmental and medical biomarkers is ever expanding due to the current climate. This push for the detection of vital biomarkers at lower concentrations, with increased reliability, has necessitated the utilisation of micro- and nano-dimensional materials. There is a wide variety of nanomaterials available for exploration, all having unique sets of properties that help to enhance the performance of biosensors. In recent years, a large portion of research has focussed on combining these different materials to utilise the different properties in one sensor platform. This research has allowed biosensors to reach new levels of sensitivity, but we note that there is room for improvement in the reporting of this field. Numerous examples are published that report improvements in the biosensor performance through the mixing of multiple materials, but there is little discussion presented on why each nanomaterial is chosen and whether they synergise well together to warrant the inherent increase in production time and cost. Research into micro-nano materials is vital for the continued development of improved biosensing platforms, and further exploration into understanding their individual and synergistic properties will continue to push the area forward. It will continue to provide solutions for the global sensing requirements through the development of novel materials with beneficial properties, improved incorporation strategies for the materials, the combination of synergetic materials, and the reduction in cost of production of these nanomaterials. Graphical abstract

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Section: Carbon Nanotube-based Biosensorsmentioning

confidence: 99%

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Crapnell

Banks

2021

Microchim Acta

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“…The experimental data were consistent with the results of clinical analysis. 76 Next is a sensor based on graphene materials. In 2013, Ye et al 77 reported a novel non-enzymatic amperometric glucose sensor based on a copper oxide nanoneedle/graphene/carbon nanofiber modified glass carbon electrode.…”

Section: Saliva Detectionmentioning

confidence: 99%

“…72 (b) Schematic diagram of the stepwise fabrication of the biosensor. 76 (c) Fabrication steps of a BSA/anti-CYFRA-21-1/APTES/nHfO 2 /ITO platform for oral cancer detection. 82 (d) Fabrication process and constituents of the reagent layer of the working electrode.…”

Section: Studies Of Tear Detection Mostly Focus On Contact Lens Sensorsmentioning

confidence: 99%

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Recent advances in biofluid detection with micro/nanostructured bioelectronic devices

Zhang

et al. 2021

Nanoscale

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“…Most sensors have a modifying composite layer that may contain various materials. Sometimes the process of creating a multicomponent modifier and composite layers is multi-staged and time-consuming [19][20][21][22] and requires the use of harmful solvents [21,23]. In this regard, the search for original approaches and new electrically conductive materials with highly developed active surfaces, which can be used for creating simple and highly sensitive sensors for UA determination is relevant.…”

Section: Introductionmentioning

confidence: 99%

Developing Activated Carbon Veil Electrode for Sensing Salivary Uric Acid

et al. 2021

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The paper describes the development of a carbon veil-based electrode (CVE) for determining uric acid (UA) in saliva. The electrode was manufactured by lamination technology, electrochemically activated and used as a highly sensitive voltammetric sensor (CVEact). Potentiostatic polarization of the electrode at 2.0 V in H2SO4 solution resulted in a higher number of oxygen and nitrogen-containing groups on the electrode surface; lower charge transfer resistance; a 1.5 times increase in the effective surface area and a decrease in the UA oxidation potential by over 0.4 V, compared with the non-activated CVE, which was confirmed by energy dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, chronoamperometry and linear sweep voltammetry. The developed sensor is characterized by a low detection limit of 0.05 µM and a wide linear range (0.09–700 µM). The results suggest that the sensor has perspective applications for quick determination of UA in artificial and human saliva. RSD does not exceed 3.9%, and recovery is 96–105%. UA makes a significant contribution to the antioxidant activity (AOA) of saliva (≈60%). In addition to its high analytical characteristics, the important advantages of the proposed CVEact are the simple, scalable, and cost-effective manufacturing technology and the absence of additional complex and time-consuming modification operations.

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An electrochemical biosensor based on multi-wall carbon nanotube–modified screen-printed electrode immobilized by uricase for the detection of salivary uric acid

Cited by 55 publications

References 39 publications

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Recent advances in biofluid detection with micro/nanostructured bioelectronic devices

Developing Activated Carbon Veil Electrode for Sensing Salivary Uric Acid

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