An ELISA for Detection of Antibodies to the E2 Protein of GB Virus C

Dille, Bruce J.; Surowy, Teresa K.; Gutierrez, Robin A.; Coleman, Paul F.; Knigge, Mark F.; Carrick, Robert J.; Aach, Richard D.; Hollinger, F. Blaine; Stevens, Cladd E.; Barbosa, Luiz H.; Nemo, George J.; Mosley, James W.; Dawson, George J.; Mushahwar, Isa K.

doi:10.1093/infdis/175.2.458

Cited by 172 publications

(156 citation statements)

References 16 publications

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“…Satisfactory results have been obtained using Enzyme Immuno Assay (EIA) based on recombinant E2 protein expressed on Chinese Hamster Ovarian (CHO) cell. 8,11 HGV is transmitted by parenteral 12 , sexual 13 , and vertical 14 routes. Depending on the route of transmission of HGV, various risk groups including intravenous drug users (IDUs), commercial sex workers (CSW), multiply transfused patients (MTP), hemodialysis patients, anti-HCV positive patients, and anti-HIV positive patients were considered as study group in the present study.…”

Section: Resultmentioning

confidence: 99%

Low Prevalence of Antibody to Hepatitis G Virus Among the Risk Groups and Healthy Population of Bangladesh

Siddiqua¹,

Nawsher²,

Tabassum

et al. 2012

Bangladesh J Med Microbiol

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Dr Mahmuda Siddiqua E-mail : mahmuda99@yahoo.com hepatitis, occurring in patients with no other known etiology. 5It is well established that HGV maintains a high and stable level of plasma viremia for many years, 6 which indicates it's efficient and active replication. Before the establishment of antibody detection methods, prevalence rates of HGV RNA in general population varied between less than 1 to 4% in different countries.7 With the development of new tests for detection of anti-HGV antibodies about 3 to 20 % of healthy people are found to have antibodies, indicating that the virus is more widely spread worldwide. In particular, rates of HGV seropositivity have ranged between 2-8% in Asia and North America, between 10-15 % in Europe and around 20% in South Africa and South America.

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Section: Resultmentioning

confidence: 99%

Low Prevalence of Antibody to Hepatitis G Virus Among the Risk Groups and Healthy Population of Bangladesh

Siddiqua¹,

Nawsher²,

Tabassum

et al. 2012

Bangladesh J Med Microbiol

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“…Thereby it was shown that most patients with posttransfusion GBV-C viremia who cleared the viral RNA became anti-E2 antibody (anti-E2) positive. 7,8 Additionally, GBV-C RNA and anti-E2 were almost mutually exclusive in patients' sera indicating that anti-E2 positivity correlated with GBV-C exposure and its clearance. 7,8 GBV-C has a parenteral route of transmission, and is frequently transmitted between intravenous drug abusers.…”

mentioning

confidence: 94%

“…7,8 Additionally, GBV-C RNA and anti-E2 were almost mutually exclusive in patients' sera indicating that anti-E2 positivity correlated with GBV-C exposure and its clearance. 7,8 GBV-C has a parenteral route of transmission, and is frequently transmitted between intravenous drug abusers. 9 Genomic sequence identity has been documented in matched pairs of blood donors and recipients.…”

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confidence: 94%

Antibodies against the GB virus C envelope 2 protein before liver transplantation protect against GB virus C de novo infection

et al. 1998

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GB virus C (GBV-C) is a newly discovered RNA virus related to the Flaviviridae family. Although GBV-C is not yet associated with any cause of liver disease, a humoral immune response against the GBV-C envelope 2 (E2) protein has been observed. Therefore, we studied the prevalence and clinical relevance of GBV-C RNA and anti-E2 antibodies in patients undergoing orthotopic liver transplantation (OLT). In addition, we tested whether the prevalence of anti-E2 antibodies may protect against GBV-C infection. Of the 182 liver recipients included in this study, 117 of these were evaluated for GBV-C recurrence or de novo infection. GBV-C RNA was detected in sera or plasma using single-tube, reverse-transcriptase polymerase chain reaction, and anti-E2 antibody was detected by enzyme immunoassay (EIA). Cumulative patient and graft survival was tested by using Kaplan-Meier analysis. The independence of prognostic values was assessed by using Cox regression analysis. Before OLT, GBV-C RNA and anti-E2 were detected in 4.0% to 28.6% and 10.0% to 68.8%, respectively, of patients suffering from different forms of chronic liver diseases. GBV-C reinfection after OLT was determined in 85.7%. Of the patients without evidence of exposure to GBV-C before OLT, 30 of 65 (46.2%) became GBV-C RNA positive after OLT. None of the 38 patients who were anti-E2 antibody positive before OLT became GBV-C RNA positive after OLT. Neither patient nor graft survival was significantly affected by the presence of either GBV-C RNA or anti-E2 antibody before OLT. Our data indicate that 1) GBV-C RNA positive patients have a high risk of reinfection after OLT, and 2) the presence of anti-E2 antibodies before OLT is associated with an absence of GBV-C infection after OLT, which may indicate a protective role of anti-E2 antibodies. (HEPATOLOGY 1998;28:379-384.)

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“…Since the end of 1996, an ELISA has been available for detection of antibodies to an envelope protein (anti-E2) of GBV-C (Tacke et al, 1997). Anti-E2 seroconversion is associated with viral clearance, suggesting that anti-E2 antibodies are a marker of a previous GBV-C infection (Dille et al, 1997). Therefore combining RT-PCR and ELISA should render a higher, more realistic, prevalence of GBV-C in various populations.…”

mentioning

confidence: 99%

Virus inactivation and prevalence of GBV‐C in haemophiliacs

Uhle

Zimmermann²,

Goeser³

et al. 1997

Br J Haematol

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Summary.A new putative hepatitis virus has recently been discovered and termed GB virus C (GBV-C). We investigated the prevalence of this virus among 50 haemophiliacs treated with non-virus-inactivated clotting factor concentrates prior to 1985 and 21 haemophiliacs treated exclusively with virus-inactivated clotting factor concentrates. In the first group the prevalence of GBV-C based on PCR and ELISA was 46%. In the second group the prevalence of GBV-C was similar to that of healthy blood donors (5%). We therefore conclude that GBV-C is reliably inactivated by modern virucidal methods such as vapour heating, pasteurization and treatment with solvent/detergent mixtures.

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An ELISA for Detection of Antibodies to the E2 Protein of GB Virus C

Cited by 172 publications

References 16 publications

Low Prevalence of Antibody to Hepatitis G Virus Among the Risk Groups and Healthy Population of Bangladesh

Low Prevalence of Antibody to Hepatitis G Virus Among the Risk Groups and Healthy Population of Bangladesh

Antibodies against the GB virus C envelope 2 protein before liver transplantation protect against GB virus C de novo infection

Virus inactivation and prevalence of GBV‐C in haemophiliacs

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