An inactivated vaccine for contagious caprine pleuropneumonia

Rurangirwa, F. R.; McGuire, T C; Kibor, A; Chema, S.

doi:10.1136/vr.121.17.397

Cited by 48 publications

(29 citation statements)

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“…A reference batch of CCPP vaccine was produced by CIRAD, as described by Rurangirwa [16], but with slight modification of the published protocol, including inactivation before freeze-drying. Briefly, the Mccp antigen consisted of a four-day culture of the “Abomsa” strain.…”

Section: Methodsmentioning

confidence: 99%

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An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

et al. 2014

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BackgroundFew serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the “mycoides cluster” frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study.ResultsThe strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the “mycoides cluster”. Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals.ConclusionsThis newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.

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Section: Methodsmentioning

confidence: 99%

“…The vaccines against CCPP consist of inactivated Mccp antigen with saponin as an adjuvant [16]. If effective, these vaccines should induce marked seroconversion, and this need-s to be investigated furher.…”

Section: Introductionmentioning

confidence: 99%

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

et al. 2014

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“…Current mycoplasma vaccines are generally either live or inactivated preparations of whole organisms (10,23,29,34). There has been little success with subunit vaccines or recombinant proteins (35), and this is especially true for CBPP, for which the only Office International des Epizootes-recommended vaccines are freeze-dried live formulations (strain T 1 44 or its streptomycin-resistant derivative T 1 SR).…”

Section: Discussionmentioning

confidence: 99%

Phage Library Screening for the Rapid Identification and In Vivo Testing of Candidate Genes for a DNA Vaccine against Mycoplasma mycoides subsp. mycoides Small Colony Biotype

et al. 2006

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A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage ZAP Express vector which contains both prokaryotic (P lac ) and eukaryotic (P CMV ) promoters upstream of the insertion site. The phage library is plated on Escherichia coli cells, immunoblotted, and probed with hyperimmune and/or convalescent-phase antiserum to rapidly identify vaccine candidates. These are then plaque purified and grown as liquid lysates, and whole bacteriophage particles are then used directly to immunize the host, following which P CMV -driven expression of the candidate vaccine gene occurs. In the example given here, a semirandom genome library of the bovine pathogen Mycoplasma mycoides subsp. mycoides small colony (SC) biotype was cloned into ZAP Express, and two strongly immunodominant clones, -A8 and -B1, were identified and subsequently tested for vaccine potential against M. mycoides subsp. mycoides SC biotype-induced mycoplasmemia. Sequencing and immunoblotting indicated that clone -A8 expressed an isopropyl-␤-D-thiogalactopyranoside (IPTG)-inducible M. mycoides subsp. mycoides SC biotype protein with a 28-kDa apparent molecular mass, identified as a previously uncharacterized putative lipoprotein (MSC_0397). Clone -B1 contained several full-length genes from the M. mycoides subsp. mycoides SC biotype pyruvate dehydrogenase region, and two IPTG-independent polypeptides, of 29 kDa and 57 kDa, were identified on immunoblots. Following vaccination, significant anti-M. mycoides subsp. mycoides SC biotype responses were observed in mice vaccinated with clones -A8 and -B1. A significant stimulation index was observed following incubation of splenocytes from mice vaccinated with clone -A8 with whole live M. mycoides subsp. mycoides SC biotype cells, indicating cellular proliferation. After challenge, mice vaccinated with clone -A8 also exhibited a reduced level of mycoplasmemia compared to controls, suggesting that the MSC_0397 lipoprotein has a protective effect in the mouse model when delivered as a bacteriophage DNA vaccine. Bacteriophage-mediated immunoscreening using an appropriate vector system offers a rapid and simple technique for the identification and immediate testing of putative candidate vaccines from a variety of pathogens.Whole bacteriophage (phage) particles have recently been described as highly efficient DNA vaccine delivery vehicles (6,7,17,20), inducing significant antibody responses in both laboratory mice (7) and larger animals such as rabbits (20) and sheep (33). Bacteriophages are viruses of bacteria and are metabolically inert, requiring the host bacterium for growth and propagation. Using standard "naked" DNA vaccination, purified genetic material in the form of a plasmid encoding protective antigens is given to the host and used to stimulate an immune response and give protection against pathogens (37,42). With bacteriophage genetic immunization, expression cassettes containing a vaccine gene under the control of a su...

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“…18 The possible occurrence of CCPP in wild herbivores in contact with livestock has been investigated in Kenya. 17 Isolation of mycoplasmas other than Mccp has been reported in other wild species such as the Rocky mountain bighorn sheep (Ovis Canadensis), mouflon (Ovis musimon), aoudad (Ammotragus lervi), 14,23 and Vaal rhebok (Pelea capreolus). 13 perimental infection of a single Thompson's gazelle (Gazella thompsoni) with Mycoplasma mycoides subsp.…”

Section: Introductionmentioning

confidence: 97%

Contagious Caprine Pleuropneumonia Outbreak in Captive Wild Ungulates at Al Wabra Wildlife Preservation, State of Qatar

Arif¹,

Schulz²,

Thiaucourt³

et al. 2007

Journal of Zoo and Wildlife Medicine

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Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae is a highly contagious and serious respiratory disease of domestic goats, characterized by coughing, severe respiratory distress, and high mortality rates. The lesions at necropsy are mainly a fibrinous pleuropneumonia with increased straw-colored pleural fluid. An outbreak of CCPP in wild goat (Capra aegagrus), Nubian ibex (Capra ibex nubiana), Laristan mouflon (Ovis orientalis laristanica), and gerenuk (Litocranius walleri) occurred at Al Wabra Wildlife Preservation in the State of Qatar. The disease was suspected because of the clinical symptoms and the necropsy findings and was confirmed by the isolation and identification of the causative organism. This new finding indicates that CCPP should be considered a potential threat to wildlife and the conservation of endangered ruminant species, especially in the Middle East, where it is enzootic because of its presence in chronic carriers. Susceptible imported animals should be quarantined and vaccinated. The preferred samples for diagnosis are the pleural fluid, which contains high numbers of Mycoplasma, and sections of hepatized lung, preferably at the interface of normal and diseased tissues. Samples must be shipped to diagnostic laboratories rapidly, and appropriate cool conditions must be maintained during shipping.

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An inactivated vaccine for contagious caprine pleuropneumonia

Cited by 48 publications

References 0 publications

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

Phage Library Screening for the Rapid Identification and In Vivo Testing of Candidate Genes for a DNA Vaccine against Mycoplasma mycoides subsp. mycoides Small Colony Biotype

Contagious Caprine Pleuropneumonia Outbreak in Captive Wild Ungulates at Al Wabra Wildlife Preservation, State of Qatar

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