An ultra-sensitive aptasensor based on carbon nanohorns/gold nanoparticles composites for impedimetric detection of carbendazim at picogram levels

Zhu, Chengxi; Liu, Dong; Chen, Zhi; Li, Libo; You, Tianyan

doi:10.1016/j.jcis.2019.03.035

Cited by 77 publications

(22 citation statements)

References 40 publications

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“…In the I gs curve, we observe that the peak attributed to the electron transfer process between gold and graphene (peak (2) at 0.1 V) before the aptamer functionalization is then replaced by a broad and less intense peak centered at 0.3 V (peak (3)). The broadness and the shift to higher voltage values are presumably the result of the change in the electron-transfer kinetics mediated by AuNPs, due to the self-assembly of negatively charged aptamers on the electrode surface 51,52 in combination with the formation of a competitive electron transfer process between ferrocene (Fc) and graphene 53,54 . The aptamer immobilization on the decorated electrode impedes the electron exchange between AuNPs and the electrode 52 , promoting the transfer process borne by the Fc label.…”

Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

“…The broadness and the shift to higher voltage values are presumably the result of the change in the electron-transfer kinetics mediated by AuNPs, due to the self-assembly of negatively charged aptamers on the electrode surface 51,52 in combination with the formation of a competitive electron transfer process between ferrocene (Fc) and graphene 53,54 . The aptamer immobilization on the decorated electrode impedes the electron exchange between AuNPs and the electrode 52 , promoting the transfer process borne by the Fc label. This fact represents an indirect evidence of the aptameric functionalization.…”

Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

“…This fact represents an indirect evidence of the aptameric functionalization. However, the low peak intensity suggests that the Fc label transfer cannot compensate the intrinsic screening effect due to the aptameric functionalization, whose major effect is to markedly increase the electron transfer resistance of the electrode 52,55,56 . This fact leads to a lower I ds modulation that becomes comparable with that of the bare PMLG gate electrode.…”

Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

“…3A, respectively). Steric hindrance, in fact, reduces the surface portion of gate electrode available for an effective interaction with the electrolyte, thus increasing the electron transfer resistance 52 . This marked variation in the transfer curves in terms of both shape and modulation can be considered as the direct effect of the thrombin detection.…”

Section: Thrombin Detectionmentioning

confidence: 99%

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Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Peruzzi

Battistoni

Montesarchio

et al. 2021

Sci Rep

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In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.

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Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

Section: Pmlg-gold Nanoparticles-aptamersmentioning

confidence: 99%

Section: Thrombin Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Peruzzi

Battistoni

Montesarchio

et al. 2021

Sci Rep

View full text Add to dashboard Cite

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“…Binding of kanamycin present in pork meat and chicken liver with aptamer induces decrease in DPV current [ 16 ]. Carbendazim present in the lettuce and orange juice sample binds to the surface of AuNPs and impedes the electron transfer to carbon nanohorns/aptamer-AuNP nanocomposite–coated electrode [ 43 ••]. Ampicillin present in the human serum, river water, and milk samples binds to aptamer-nanosheet–modified gold electrode that leads to charge transfer resistance.…”

Section: Antibiotic Detectionmentioning

confidence: 99%

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Kumar

Guleria

2020

Curr Pollution Rep

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Recent advances in nanomaterial‐assisted electrochemical sensors for food safety analysis

Ren

et al. 2022

Food Frontiers

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Food safety as a public health issue has aroused worldwide concern. Food safety risks caused by the existence of food safety hazards in food may occur in all stages of the food supply chain. Thus, great emphasis is placed on sensitive, selective, and convenient analytical methods of various food safety hazards in food. At present, nanomaterials are at the forefront of various electrochemical sensing applications. Given their unique physical, chemical, and biological characteristics, nanomaterials are considered perfect candidates for the design of electrode surface to construct electrochemical sensors with excellent analytical performance. With the assistance of nanomaterials, electrochemical sensors are a potential alternative to conventional methods of food safety analysis. This review focuses on current research achievements of nanomaterial‐assisted electrochemical sensors for food safety analysis reported in recent 5 years. It highlights the different detection mechanisms for analytes, flexible sensing strategies based on nanomaterials, and portable miniaturized electrochemical devices. Finally, the challenges and limitations in the design of electrochemical sensors for food safety analysis are also discussed.

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An ultra-sensitive aptasensor based on carbon nanohorns/gold nanoparticles composites for impedimetric detection of carbendazim at picogram levels

Cited by 77 publications

References 40 publications

Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Recent advances in nanomaterial‐assisted electrochemical sensors for food safety analysis

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