Anaerobic Transfer of Antibiotic Resistance from
            <i>Pseudomonas aeruginosa</i>

Graves, James F.; Riggs, H. G.

doi:10.1128/aem.40.1.1-6.1980

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Anaerobic matings were performed on PCA + 0.5% KN03 for 24 h at 25 "C in an anaerobic cabinet with: change in its transfer frequency ( Table 5). This contrasts with a previous report (Graves & Riggs, 1980) that RP1 transfer frequencies from P . aeruginosa to E. coli were 400-fold lower in anaerobic matings compared to aerobic.…”

Section: Eflects Of Anaerobiosis On Plasmid Transfercontrasting

confidence: 99%

“…Transfer frequencies of RP 1 have been reported to reach a maximum after 1 h (Lacy & Leary, 1975) and 24 h (Graves & Riggs, 1980), whereas our results show a maximum frequency after 5 h (Fig. 3 b).…”

Section: Eflect Of Mating Time On Plasmid Transfercontrasting

confidence: 77%

“…It has been reported previously that plasmid transfer frequency is affected by the ratio of donors and recipients in the mating. Gauthier et al (1985) reported that the majority of plasmids from mercury-resistant marine isolates transferred maximally at donor to recipient ratios of 1, and Graves & Riggs (1980) reported that RP1 transfer frequencies were higher at initial ratios 2 15 than at ratios Q 1. Our results show maximum transfer frequencies of pQM1, pQM3, and RP1 at initial donor to recipient ratios of 04-30, depending on the plasmid, but all three plasmids transferred over the range of ratios used (Fig.…”

Section: Efect Of the Ratio Of Donors And Recipients On Plasmid Transfermentioning

confidence: 99%

“…0001-4815 0 1989 SGM investigated the influence of various factors on conjugal plasmid transfer. The effects of temperature and pH have been studied (Shenderov, 1971;Singleton 8z Anson, 1981, 1983Altherr & Kasweck, 1982;Kelly & Reanney, 1984;Gauthier et al, 1985) but few reports have dealt with the effects of mating time, nutrient status, or donor to recipient ratio (Beringer, 1974;Gauthier et al, 1985), and reports on the effect of anaerobiosis on conjugation have been conflicting (Anderson, 1975;Burman, 1975;Graves & Riggs, 1980). Most of these studies used pure cultures of bacteria: genetic work with mixed natural communities is rare (Bale et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Factors Affecting Conjugal Transfer of Plasmids Encoding Mercury Resistance from Pure Cultures and Mixed Natural Suspensions of Epilithic Bacteria

1989

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Sixty-five pure cultures of epilithic bacteria were examined for their ability to transfer mercury resistance to Pseudomonas aeruginosa; five isolates transferred plasmids encoding mercury resistance with frequencies ranging from 8.4 x 10(-8) to 2.8 x 10(-3) per recipient. Two of the plasmids, pQM3 and pQM4, encoded narrow-spectrum mercury resistance, pQM3 also encoded streptomycin resistance, and both plasmids were broad host range. Maximum transfer frequencies of epilithic plasmids from pure cultures occurred over the range 10-25 degrees C at 3.5 g C l-1 and with donor to recipient ratios of 0.4-30. Transfer occurred over a range of pH values (pH 5.0-8.0) but the effect of pH was most significant at non-optimal temperature. Anaerobiosis inhibited transfer of one epilithic plasmid, pQM1, but not that of pQM3. Plasmids encoding mercury resistance were also transferred from mixed natural suspensions of epilithic bacteria (MNS) to Pseudomonas spp. on agar in the laboratory. Transfer from MNS occurred over a wide range of environmentally relevant conditions with maximum frequencies (2 x 10(-5) per recipient) after 24 h, at 25 degrees C, pH 5.5-8.0 and on a medium containing 10 g C l-1. The optimal initial cell density of MNS and recipient was 1.7 x 10(5) c.f.u. cm-2 and highest frequencies were obtained with donor to recipient ratios ranging from 1.2 x 10(-1) to 1.7 x 10(-3). Most of the plasmids (54%) from MNS transferred from their original P. aeruginosa transconjugants to a Pseudomonas putida strain, with frequencies ranging from 1.1 x 10(-6) to greater than 1.0 x 10(-1) per recipient. The majority (80%) of the plasmids were larger than 300 kb and all of these large plasmids encoded UV resistance in addition to mercury resistance. Twenty-one plasmids greater than 300 kb were analysed by restriction digests and were shown to be similar, with only minor structural alterations. One of these alterations was associated with the acquisition of streptomycin resistance. Overall, these results suggest that the epilithic bacteria examined possess the potential to transfer mercury resistance within the epilithon under a wide range of environmentally relevant conditions.

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Section: Eflects Of Anaerobiosis On Plasmid Transfercontrasting

confidence: 99%

Section: Eflect Of Mating Time On Plasmid Transfercontrasting

confidence: 77%

Section: Efect Of the Ratio Of Donors And Recipients On Plasmid Transfermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Factors Affecting Conjugal Transfer of Plasmids Encoding Mercury Resistance from Pure Cultures and Mixed Natural Suspensions of Epilithic Bacteria

1989

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“…Similar effects of temperature ( 50 , 51 ) and anaerobiosis ( 50 ) were previously shown for IncP1 mercury resistance conjugative plasmids. Decrease of conjugation transfer at 37°C compared to 30°C was also demonstrated for the IncHI plasmid R27 ( 52 ), while lower frequencies of RP1 plasmid conjugation from Pseudomonas aeruginosa to E. coli was also observed at anaerobic versus aerobic conditions ( 53 ).…”

Section: Discussionmentioning

confidence: 84%