Analisis Keragaman Genetik 28 Nomor Koleksi Kakao  (Theobroma cacao L.) Berdasarkan Marka SSR

Wicaksono, Ilham Nur Ardhi; Rubiyo, Rubiyo; Sukma, Dewi; Sudarsono, Sudarsono

doi:10.21082/jtidp.v4n1.2017.p13-22

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…Those combinations are expected to produce progenies with more agronomically favorable characteristics than their parents. The use of genetic distance values to determine promising parental clones for hand pollination has also been done in previous studies on cacao and coffee (Rubiyo et al, 2015;Izzah et al, 2015;Dani et al, 2016;and Wicaksono et al, 2017). In addition, the grouping of cacao clones using SSR markers may help breeders in selecting promising clones as candidates for new varieties.…”

Section: Genetic Variability and Relationship Of 20 Cacao Clonesmentioning

confidence: 96%

“…This was shown by the ability of these markers to distinguish among local cacao clones as well as between local clones and national varieties. On the other hand, high values of heterozygosity can be attributed to the cross-pollinated nature of cacao that allows mixing of multiple alleles (Wicaksono et al, 2017). Meanwhile, the high variation found in gene diversity and PIC indicated the high diversity of local cacao clones collected from Lima Puluh Kota Regency.…”

Section: Variability Of Alleles Generated From Ssr Marker Genotypingmentioning

confidence: 99%

“…Clustering dendrogram results separated these clones into two major groups. Moreover, the latest study successfully clustered 28 cacao clones into three groups using 20 polymorphic SSR markers (Wicaksono, Rubiyo, Sukma, & Sudarsono, 2017). The aim of this study was to assess genetic variability of 11 local cacao clones collected from Lima Puluh Kota Regency, West Sumatra, and 9 national varieties as references using SSR markers.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Variability of 11 Local Cacao Clones Derived from West Sumatra Using SSR Markers

Tresniawati¹,

Izzah²,

Sulistiyorini³

et al. 2017

J. tanam. industri dan penyegar

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ABSTRAKIndonesia is the third largest cacao producing-country in the world and known having many superior local clones, such as that found in Lima Puluh Kota Regency, West Sumatra. However, there is lack of information about genetic background of those local cacao clones. This study aimed to assess genetic variability of 11 local cacao clones collected from Lima Puluh Kota Regency, West Sumatra using SSR markers. The research was conducted in the Integrated Laboratory, Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from August to November 2016. The genetic variabilities of local cacao studied were compared with 9 national varieties as reference genomes. Total genomic DNA of the plants was isolated using CTAB method. Cacao DNA was amplified using 18 SSR markers to determine their genetic variability. Afterward, the amplified DNA was separated using 6% non-denaturing polyacrylamide gel electrophoresis. The result exhibited that 12 markers were polymorphic. Further analysis of these polymorphic markers using PowerMarker program revealed a total of 83 alleles were obtained from all cacao clones analyzed. Meanwhile, PIC values ranged from 0.55 to 0.86 with an average of 0.70. A genetic similarity matrix based on UPGMA revealed three main groups at 68% similarity coefficients. Interestingly, all of the 11 local cacao clones were clearly distinguished each other and also from the national varieties. The result demonstrated the usefulness of SSR markers for discriminating local cacao clones.Further study is required to use these local clones in cacao breeding programs.Keywords: Genetic variability, local clones, SSR markers, Theobroma cacao L. ABSTRACT Indonesia adalah negara produsen kakao terbesar ketiga di dunia yang memiliki banyak genotipe kakao unggul lokal, seperti yang terdapat di Kabupaten Lima Puluh Kota, Sumatera Barat. Namun demikian, hanya sedikit informasi tentang latar belakang genetik dari kakao lokal tersebut yang diketahui. Tujuan penelitian adalah untuk menilai keragaman genetik 11 klon kakao lokal yang dikoleksi dari Kabupaten Lima Puluh Kota, Sumatera Barat menggunakan marka SSR. Penelitian dilaksanakan di Laboratorium Terpadu Balai Penelitian Tanaman Industri dan

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Section: Genetic Variability and Relationship Of 20 Cacao Clonesmentioning

confidence: 96%

Section: Variability Of Alleles Generated From Ssr Marker Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Variability of 11 Local Cacao Clones Derived from West Sumatra Using SSR Markers

Tresniawati¹,

Izzah²,

Sulistiyorini³

et al. 2017

J. tanam. industri dan penyegar

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“…Hasil analisis menggunakan 12 marka SSR menghasilkan alel sebanyak 45 alel (Tabel 2), dengan rata-rata jumlah alel sebanyak 3,5 per lokus. Jumlah alel yang dihasilkan pada penelitian ini berbeda dengan hasil penelitian sebelumnya, meskipun menggunakan marka SSR yang sama (Kurniasih, Rubiyo, Setiawan, & Sudarsono, 2011;Rubiyo, Izzah, Sulistiyorini, & Tresniawati, 2015); Wicaksono, Rubiyo, Sukma, & Sudarsono, 2017). Perbedaan jumlah alel yang dihasilkan dengan penelitian sebelumnya diduga disebabkan perbedaan jenis populasi yang digunakan dalam penelitian.…”

Section: Karakteristik Marka Ssrunclassified

Evaluation of Clonal Uniformity in Six Superior Cacao Clones Based on SSR Marker

Sulistiyorini

Rubiyo

Sudarsono

2018

J. tanam. industri dan penyegar

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Propagation of cacao plants is generally carried out vegetatively. Therefore, plants that are clonally propagated should be genetically uniform. Genetic uniformity in cacao clones is also very important information for germplasm conservation and in obtaining pure parental crosses. Evaluation of genetic uniformity can be seen through analysis using SSR markers. This study aimed to determine the genetic uniformity in six cacao clones using SSR markers. This experiment was conducted at IIBCRI Integrated Laboratory in Sukabumi and Plant Molecular Biology Laboratory, IPB Bogor, from September 2015 to December 2016. Six cacao clones used (TSH 858, TSH 908, ICS 13, PA 300, GC 7 and UIT) are from Kalitelepak experimental station of PTPN XII, Genteng District, Banyuwangi, East Java. Ten samples were taken randomly to represent cacao clones. DNA amplification was carried out using 12 SSR markers. The result showed that 12 SSR markers generated 45 alleles with the number of alleles per locus was 3-4 alleles. The polymorphic information content (PIC) ranges from 0.37–0.67, which are identied as very informative molecular analysis in identifying the genetical uniformity of the evaluated cacao population. Six SSR loci generated variant alleles within both the TSH 858 and UIT clones, indicating there are off-type plants in these two samples. Clonal uniformity were detected for samples of the GC 7, ICS 13, PA 300 and TSH 908 clones. On the other hand, 8.33% of evaluated samples within the TSH 858 and UIT clones were off-type plants.

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RAPD Amplification on Cocoa (Theobroma cacao L.) From East Kolaka, Southeast Sulawesi Province

Syahri

Rauf

Paembonan

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

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Analisis Keragaman Genetik 28 Nomor Koleksi Kakao (Theobroma cacao L.) Berdasarkan Marka SSR

Cited by 3 publications

References 10 publications

Genetic Variability of 11 Local Cacao Clones Derived from West Sumatra Using SSR Markers

Genetic Variability of 11 Local Cacao Clones Derived from West Sumatra Using SSR Markers

Evaluation of Clonal Uniformity in Six Superior Cacao Clones Based on SSR Marker

RAPD Amplification on Cocoa (Theobroma cacao L.) From East Kolaka, Southeast Sulawesi Province

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