Analysis of differentially expressed genes in oral epithelial cells infected with <i>Fusobacterium nucleatum</i> for revealing genes associated with oral cancer

Zhang, Shuwei; Li, Chen; Zhang, Zhiying; Li, Yuchao; Li, Qian; Geng, Fei; Liu, Junchao; Pan, Yaping

doi:10.1111/jcmm.16142

Cited by 25 publications

(17 citation statements)

References 61 publications

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“…For example, the placental microbiome profiles were most comparable to those of the oral microbiome [ 1 ]. The overlap of the unique members of oral microbes with other remote organs is consistent with previous clinical studies in which Fusobacterium nucleatum , a Gram-negative oral anaerobe, were clinically suspected to be a major risk factor in colorectal cancer [ 97 , 98 , 99 ], oral squamous cell carcinoma (OSCC) [ 100 ], and in preterm and term stillbirth [ 101 , 102 ]. Likewise, an infamous oral pathogenic bacterium, Porphyromonas gingivalis , is related to pancreatic cancer [ 103 ], colorectal cancer [ 104 , 105 , 106 ], liver health [ 107 ], rheumatoid arthritis [ 108 , 109 ], diabetes [ 110 , 111 , 112 ], OSCC [ 113 , 114 ], and neurodegenerative diseases [ 88 , 89 , 90 , 91 , 92 , 115 , 116 , 117 , 118 ].…”

Section: Oral Pathogens and Systemic Diseasessupporting

confidence: 86%

Leaky Gum: The Revisited Origin of Systemic Diseases

Park¹,

Park²,

Lee³

et al. 2022

Cells

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The oral cavity is the gateway for microorganisms into your body where they disseminate not only to the directly connected respiratory and digestive tracts but also to the many remote organs. Oral microbiota, travelling to the end of the intestine and circulating in our bodies through blood vessels, not only affect a gut microbiome profile but also lead to many systemic diseases. By gathering information accumulated from the era of focal infection theory to the age of revolution in microbiome research, we propose a pivotal role of “leaky gum”, as an analogy of “leaky gut”, to underscore the importance of the oral cavity in systemic health. The oral cavity has unique structures, the gingival sulcus (GS) and the junctional epithelium (JE) below the GS, which are rarely found anywhere else in our body. The JE is attached to the tooth enamel and cementum by hemidesmosome (HD), which is structurally weaker than desmosome and is, thus, vulnerable to microbial infiltration. In the GS, microbial biofilms can build up for life, unlike the biofilms on the skin and intestinal mucosa that fall off by the natural process. Thus, we emphasize that the GS and the JE are the weakest leaky point for microbes to invade the human body, making the leaky gum just as important as, or even more important than, the leaky gut.

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Section: Oral Pathogens and Systemic Diseasessupporting

confidence: 86%

Leaky Gum: The Revisited Origin of Systemic Diseases

Park¹,

Park²,

Lee³

et al. 2022

Cells

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“…A study using co-culture of F. nucleatum and human immortalized oral epithelial cells (HOIECs) observed the differential expression of 353 mRNAs, and the expression of multiple oncogenes, such as CREM, CREB1 and NCOA, was upregulated. An analysis of oral cancer samples in a database indicated the same trend ( Zhang et al, 2021 ). In addition, F. nucleatum bound and activated the cell inhibitory receptor CEACAM1 on CEACAM1 + TILs and CEACAM1 + tumor cells, indicating its potential importance in modulating antitumor immunity, which helped the tumor evade immune cell attack through an additive mechanism ( Gur et al, 2019 ).…”

Section: Discussionmentioning

confidence: 94%

Metagenomic Analysis Reveals a Changing Microbiome Associated With the Depth of Invasion of Oral Squamous Cell Carcinoma

Liu

et al. 2022

Front. Microbiol.

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The relationship between oral squamous cell carcinoma (OSCC) development and the microbiome has attracted increasing attention. The depth of invasion (DOI) is an important indicator of tumor progression, staging and prognosis, and the change in the oral microbiome based on the DOI is unclear. This report describes the use of metagenomic analyses to investigate the relationship between the oral microbiome and the DOI. Forty patients in different DOI categories were recruited; 10 healthy people served as the control group. Swab samples collected from the participants were subjected to metagenomic analyses, and the oral microbial communities and their functions were investigated. The abundances of Fusobacterium nucleatum, Capnocytophaga sputigena, Porphyromonas endodontalis, and Gemella haemolysans were significantly increased in the patients compared with the controls. The abundances of some bacteria exhibited a stage-related trend. The abundances of P. endodontalis, Gemella morbillorum and G. haemolysans increased with increasing DOI. In contrast, the abundances of Prevotella melaninogenica, Haemophilus parainfluenzae and Neisseria flavescens decreased with increasing DOI. Based on receiver operating characteristic (ROC) curve analysis, eight species were found to have predictive value: Rothia mucilaginosa, P. melaninogenica, H. parainfluenzae, and N. flavescens in the healthy control group and P. endodontalis, G. morbillorum, G. haemolysans and Fusobacterium periodonticum in the high DOI group. In the functional analysis, several metabolic pathways were decreased, whereas flagellar assembly and bacterial chemotaxis showed an increasing trend as the disease progressed. Biofilm formation, flagella, lipopolysaccharide (LPS) and other virulence factors exhibited staging-related changes. These pathogenic pathways and factors had a clear correlation with specific pathogens. In particular, when OSCC progressed to the late stage, microbial diversity and functional potential changed greatly.

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“…Common risk factors for oral tumors include smoking, alcohol abuse, HPV, ultraviolet rays, etc. 77 , 78 In recent years, studies have found much important evidence that oral microbes are closely related to OSCC, including P. gingivalis , 79 F. nucleatum , 80 Treponema denticola 81 , 82 and Candida , 83 etc.…”

Section: Oral Bacteria and Cancersmentioning

confidence: 99%

Oral microbiota in human systematic diseases

et al. 2022

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Oral bacteria directly affect the disease status of dental caries and periodontal diseases. The dynamic oral microbiota cooperates with the host to reflect the information and status of immunity and metabolism through two-way communication along the oral cavity and the systemic organs. The oral cavity is one of the most important interaction windows between the human body and the environment. The microenvironment at different sites in the oral cavity has different microbial compositions and is regulated by complex signaling, hosts, and external environmental factors. These processes may affect or reflect human health because certain health states seem to be related to the composition of oral bacteria, and the destruction of the microbial community is related to systemic diseases. In this review, we discussed emerging and exciting evidence of complex and important connections between the oral microbes and multiple human systemic diseases, and the possible contribution of the oral microorganisms to systemic diseases. This review aims to enhance the interest to oral microbes on the whole human body, and also improve clinician’s understanding of the role of oral microbes in systemic diseases. Microbial research in dentistry potentially enhances our knowledge of the pathogenic mechanisms of oral diseases, and at the same time, continuous advances in this frontier field may lead to a tangible impact on human health.

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Analysis of differentially expressed genes in oral epithelial cells infected with Fusobacterium nucleatum for revealing genes associated with oral cancer

Cited by 25 publications

References 61 publications

Leaky Gum: The Revisited Origin of Systemic Diseases

Leaky Gum: The Revisited Origin of Systemic Diseases

Metagenomic Analysis Reveals a Changing Microbiome Associated With the Depth of Invasion of Oral Squamous Cell Carcinoma

Oral microbiota in human systematic diseases

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