Analysis of genetic diversity of Cucurbita ficifolia Bouché from different regions of Mexico, using AFLP markers and study of its hypoglycemic effect in mice

Moya-Hernández, A.; Bosquez-Molina, Elsa; Serrato-Díaz, Alejandra; Blancas-Flores, Gerardo; Alarcón-Aguilar, Francisco Javier

doi:10.1016/j.sajb.2018.02.409

Cited by 15 publications

(16 citation statements)

References 22 publications

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“…This is a special feature of this species. 18,36,37 This physiological characterization of C. ficifolia is considered a relevant contribution because this fruit is attracting great interest due to its hypoglycemic effect.…”

Section: Cucurbita Ficifolia Extract Chemical Characterizationmentioning

confidence: 99%

“…Consequently, a comparative study of the genetic diversity of C. ficifolia in some regions of Mexico was recently performed using amplified fragment length polymorphism (AFLP).The fruit was found to possess hypoglycemic properties regardless of the morphological characteristics and the region of Mexico where it is grown. 18 On the other hand, respiration rate is known to be an excellent indicator of the metabolic activity of plant organs and a very useful guide to the potential life of horticultural products. Breathing rates vary by stage of development, and their measurement is very important because of their association with cellular metabolism because the energy derived from respiration drives all other reactions within a cell.…”

Section: Introductionmentioning

confidence: 99%

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Hypoglycemic effect and bioactive compounds associated with the ripening stages of the Cucurbita ficifolia Bouché fruit

et al. 2020

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BACKGROUND: The fruit of Cucurbita ficifolia Bouché is known in Mexico as 'chilacayote'. The scientific interest that C. ficifolia Bouché has acquired is due to its important hypoglycemic effect. The present research aimed (i) to discover whether this hypoglycemic property is present at different stages of development of this fruit, and (ii) to characterize some bioactive compounds with antioxidant or anti-inflammatory properties. Ethylene production, respiration rate, and maturity indices were determined during fruit development. The chemical characterization of the aqueous extracts of each stage of maturity studied was determined and their hypoglycemic effects were bioassayed using groups of normal mice with diabetes induced by streptozotocin at a dose of 500 mg −1 kg −1 body weight. RESULTS: Respiration rate and ethylene production showed a typical pattern for non-climacteric fruit and the quality parameters did not show significant changes. Phenolic compounds such as gallic acid and chlorogenic acid were found to have the highest concentration at 15 days of development. Extracts at 15 days showed a hypoglycemic effect that was 11% greater than that of glibenclamide in diabetized mice. CONCLUSION: All stages of development of C. ficifolia fruit had a hypoglycemic effect; however, the aqueous extract from the fruit at 15 days of development showed a better effect than glibenclamide. This finding highlights the potential of this maturity stage, and shows that it is appropriate for inclusion in treatments of type 2 diabetes mellitus. The results also indicate that phenolic compounds are mainly responsible for this effect and not D-chiro-inositol as previously thought.

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Section: Cucurbita Ficifolia Extract Chemical Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypoglycemic effect and bioactive compounds associated with the ripening stages of the Cucurbita ficifolia Bouché fruit

et al. 2020

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“…Estudios recientes a nivel genómico en C. pepo han encontrado 48 variantes a lo largo de 15 de los 20 cromosomas de la especie que pueden explicar del 1.5 al 62.9 % de la varianza fenotípica observada en distintos caracteres de importancia agronómica, lo que nuevamente sugiere una correlación entre la variación genética y morfológica en el género (Montero-Pau et al 2017). Adicionalmente, un estudio con polimorfismos de longitud de fragmentos amplificados (AFLPs por sus siglas en inglés) en poblaciones mexicanas de C. ficifolia identificó agrupaciones relacionadas al color del fruto, uno de los caracteres analizados en el presente estudio, y al color de la semilla, sugiriendo que los fenotipos observados en las accesiones están ligados a sus genotipos, formando linajes evolutivos discretos (Moya-Hernández et al 2018). Finalmente, la coexistencia observada de distintas variedades de C. ficifolia en un solo espacio con características ambientales similares (i.e.…”

Section: Discussionunclassified

Variedades locales y criterios de selección de especies domesticadas del género Cucurbita (Cucurbitaceae) en los Andes Centrales del Perú: Tomayquichua, Huánuco

et al. 2020

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Antecedentes: Es importante entender las prácticas agrícolas y los criterios de selección que moldean la diversidad de variedades locales en plantas domesticadas. La región andina comparte con la región mesoamericana y Norteamérica los centros de origen de las cinco especies domesticadas de Cucurbita. Sin embargo, los estudios sobre diversidad biológica de estas especies son escasos en Perú en comparación con México. Preguntas: ¿Cuáles son las variedades locales de Cucurbita que reconocen, promueven y conservan los agricultores en el Distrito de Tomayquichua? ¿Qué mecanismos promueven la diversidad de estas variedades? Especies estudiadas: Cucurbita ficifolia Bouché, Cucurbita moschata Duchesne, Cucurbita maxima Duchesne. Sitio y año de estudio: Tomayquichua, Huánuco, Perú (junio de 2018 y 2019). Métodos: Se realizaron entrevistas semiestructuradas a 29 familias de agricultores y comerciantes para evaluar la diversidad de variedades locales y las prácticas de manejo de las especies del género Cucurbita. Resultados: C. ficifolia es la cucurbitácea más diversa en Tomayquichua (13 variedades) seguido de C. moschata (10 variedades), las cuales se siembran en chacras y traspatios. Se reportan siete variedades de C. maxima, con el uso predominante de una variedad comercial en cultivos intensivos. Conclusión: Las prácticas agrícolas tradicionales parecen mantener la diversidad local de C. ficifolia y C. moschata. Las variedades locales de C. maxima en Tomayquichua parecen ser desplazadas progresivamente por una variedad mejorada destinada a aumentar la producción mediante prácticas agrícolas intensivas. La alta diversidad biológica de C. ficifolia en Perú sugiere un origen andino, pero se requieren estudios genéticos adicionales para aclararlo.

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“…The cluster analysis by AFLP exhibited an 89.4-99.4% similarity in the genome of all tested Cucurbita spp. genotypes from diverse locations (Moya-Hernández et al 2018). Elshafei et al (2019b) found that genetic similarity among the faba bean genotypes ranged from 0.24 to 0.96.…”

Section: Cluster Analysis Based On Hfo-tag Markersmentioning

confidence: 99%

Using high frequency oligonucleotides-targeting active gene (HFO-TAG) markers for genetic evaluation among genotypes (Cucurbita pepo L. and C. maxima L.)

et al. 2019

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Background: Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and natural poly-phenolic flavonoid compounds. Collection and conservation of genetic variability are helpful in genetic advancement programs. Twenty-two pumpkin genotypes (21genotypes of Cucurbita pepo L. and one genotype of C. maxima L.) were collected from different regions of Saudi Arabia. Fifteen HFO-TAGhigh frequency oligonucleotide-targeting active gene markers were used to analyze genetic variability among 22 pumpkin genotypes. Results: A total of 107 alleles were detected by the 15 HFO-TAG markers, an average of 7.133 alleles per primer. Polymorphisms were found in 102 alleles, an average of 6.866 alleles per primer. The PIC values measured from all of the HFO-TAG markers were high, and ranged from 0.8940 to 0.7225, with an average 0.8212 per marker. Conclusions: The results of the cluster analysis of pumpkin genotypes were separated into seven groups according to the collection region.

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Analysis of genetic diversity of Cucurbita ficifolia Bouché from different regions of Mexico, using AFLP markers and study of its hypoglycemic effect in mice

Cited by 15 publications

References 22 publications

Hypoglycemic effect and bioactive compounds associated with the ripening stages of the Cucurbita ficifolia Bouché fruit

Hypoglycemic effect and bioactive compounds associated with the ripening stages of the Cucurbita ficifolia Bouché fruit

Variedades locales y criterios de selección de especies domesticadas del género Cucurbita (Cucurbitaceae) en los Andes Centrales del Perú: Tomayquichua, Huánuco

Using high frequency oligonucleotides-targeting active gene (HFO-TAG) markers for genetic evaluation among genotypes (Cucurbita pepo L. and C. maxima L.)

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