Analysis of pharmaceutically relevant taxoids in wild yew trees from Sardinia

Ballero, Mauro; Loi, Maria Cecilia; Rozendaal, Erik L. M. van; Beek, T.A. van; Haar, Cees Van De; Poli, Ferruccio; Appendino, Giovanni

doi:10.1016/s0367-326x(02)00301-5

Cited by 17 publications

(15 citation statements)

References 8 publications

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“…However, the contents of other taxoids such as 10-DAB III and 7-xyl-10-DAT are much higher than paclitaxel in Taxus species, and pharmacological experiments have indicated that some of them, such as 7-xyl-10-DAT, exert strong anti-tumor activity (Lataste et al, 1984). These taxoids are precursors necessary for the biosynthesis of paclitaxel in the plants (Madhusudanan et al, 2002b;Zu et al, 2006;Ballero et al, 2003;Mroczek and Glowniak, 2001). Therefore, these taxoids have become one of the hot spots in the field of paclitaxel research in recent years.…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 98%

“…Analysis of extracts from Taxus species is usually made on C 18 (Ballero et al, 2003;Nadeem et al, 2002), C 8 (Lauren et al, 1995), phenyl (Theodoridis et al, 1998;Bala et al, 1999), biphenyl (Kethchum and Gibson, 1993) and cyano phases (Auriola et al, 1992). In most studies, C 18 columns were used to analyze samples.…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

“…In previous studies, the analysis of taxoids from plant materials was performed using C 18 columns and acetonitrile or methanol with an efficacy of approximately 50% in the mobile phase (Ballero et al, 2003;Mroczek and Glowniak, 2001;Nadeem et al, 2002;Van Rozendaal et al, 2000). However, it was difficult to separate paclitaxel, 7-xyl-10-DAT, 10-DAT and 10-DAB III with C 18 columns in the mobile phase.…”

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confidence: 98%

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Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

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Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 98%

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

mentioning

confidence: 98%

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Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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“…So far, taxoids have been analyzed by TLC [17], GC, HPLC [18,19], immunoaffinity chromatography [20], highspeed countercurrent chromatography (HSCCC) [11], and MEKC [21]. And with the development of analytical methods, rapid taxoids determination by HPLC (photodiode array detector (PAD))-MS appeared [22 -26], which made the analytical time shorter and increased the veracity of analysis also.…”

Section: Introductionmentioning

confidence: 99%

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Fu²,

et al. 2006

J of Separation Science

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A method is described for the simultaneous determination of paclitaxel and three related taxoids, 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine, in the extracts from the needles of three Chinese yew species, Taxus cuspidata, T. chinensis, and T. media. SPE was applied as the sample preparation technique and RP-HPLC with a photodiode array detector (PAD) was used for the analysis of extract samples. The crude extracts were treated with an improved SPE cartridge packed with a combination of 1-vinyl-pyrrolidin-2-one and divinyl-benzene. The eluent was 75% methanol. The following separation was achieved with a gradient program on an HIQ SIL C18W column in a system of ACN/water within 60 min. The samples were detected by PAD at wavelengths of 232.1 nm for 10-DAB III, 229.8 nm for baccatin III and paclitaxel, and 223.9 nm for cephalomannine. The content of 10-DAB III, baccatin III, cephalomannine, and paclitaxel varied from 0.0277 to 0.0875, 0.0254 to 0.0405, 0.0715 to 0.2486, and 0.0996 to 0.1301 mg/g in fresh needles of the above yew species, respectively. The assay achieved good resolution in the separation between the four compounds, and it can be used for quality control or purity determination for those in bulk and pharmceutical dosage forms.

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“…Up to now, taxoids have been analyzed by many methods, including TLC [24], immunoaffinity chromatography (IAC) [25], GC, HPLC [26,27], high speed countercurrent chromatography (HSCCC) [28], and micellar electrokinetic chromatography (MEKC) [29]. With the development of analytical methods, LC-MS/MS has been accepted as a more accurate and sensitive method for the identification and determination of compounds [30,31].…”

Section: Introductionmentioning

confidence: 99%

Determination of main taxoids in Taxus species by microwave‐assisted extraction combined with LC‐MS/MS analysis

Luo

Nie

Fu³

et al. 2009

J of Separation Science

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A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows: extraction solvent 80% ethanol solution, solid/liquid ratio 1:10 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species.

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Analysis of pharmaceutically relevant taxoids in wild yew trees from Sardinia

Cited by 17 publications

References 8 publications

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Determination of main taxoids in Taxus species by microwave‐assisted extraction combined with LC‐MS/MS analysis

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