Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

Abstract

This is a preliminary study of the protein profiles of the semen in Jersey breed bulls. Jersey bulls were mainly used for fertility purposes; they were grouped based on the fertility, such as highly fertile group (95% fertility), medium fertile group (65-70%), low fertile group (45-50%) and hybrid bulls. Semen of 20 Jersey and hybrid bulls was collected through artificial vagina. The semen characteristics includes volume, pH value, viscosity, viability, sperm concentration, agglutination, total motility and their group, sperm morphology and Hypo-Osmotic Swelling test (HOS) were carried out immediately after collection. Total antioxidant capacity test by using catalase and cholesterol analysis were also carried out. Quantification of the amino acids and proteins were carried out by Ninhydrin and Lowry method, protein analysis by Sodium Dodecyl Sulphate (SDS) was carried out for the following categories. (1) Highly fertile group (Jersey), (2) Medium fertile group (Jersey), (3) Low fertile group (Jersey) and (4) Hybrid group. Analysis of semen protein reveals about seventeen protein bands of sizes ranging between 14 and 205 kDa were identified. Among those proteins bands, the relative protein content of nine bands shows significantly different from each group. The rest of the proteins bands seem to have some positive and/or negative correlation on the semen characterizes or fertility. The antioxidant capacity of highly fertile and hybrid group was very high compare to low fertile group of Jersey. This study clearly indicates the bull seminal plasma proteins are associated with fertility and as well as determination of semen quality and ultimately decides the fertility.Key words: Semen, seminal plasma, jersey bulls, catalase, antioxidants, ninhydrin test INTRODUCTIONSemen consists of mature spermatozoon and swims in a highly complex features seminal fluid. Seminal fluid contains a complex mixture of organic compounds secreted from epididymis, testes and other accessory sex glands, influences sperm morphology, motility, sperm concentration acrosome reaction and fertility (Mann and Lutwak-Mann, 1981). Biochemical analysis of seminal fluid reveals that it is composed of amino acids, proteins and polypeptides. These molecules of seminal plasma determines semen quality and fertility were different in different species, some are reported to be associated with the fertility. Good quality semen is the most important factor to implement breeding programs (Stradaioli et al., 2004).The presence of proteins, lipids and hormones in seminal plasma suggests that a large part of the physiological functions of this fluid are not yet ascertained. It is a fact that components especially, high levels of fructose and zinc are not present in other body fluids. Effective semen

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

“…Bulls with high fertility demonstrate a high antioxidant capacity of seminal plasma protein [35,36]. The 150.29-kDa band of the Simmental bull seminal plasma (Figure -1) was verified as an IGF-I complex on Western blotting using IGF-1 monoclonal antibody (Figure -2).…”