Analysis of the kinetic characteristics of lactate dehydrogenase from the rat brain during ischemia and reperfusion

Халилов, Р. А.; Dzhafarova, A. M.; Dzhabrailova, R. N.; Emirbekov, E Z

doi:10.1134/s1819712414040047

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…(11) In one study, an increase was found in rat brain LDH activity under occlusion of the carotid artery for 1 hour. (12) Similarly, in another study, there was an increase in the LDH activity in pyramidal neurons of the hippocampus and layers II, III and V of the cerebral cortex of the Mongolian gerbils after 7 minutes of ischemia. The LDH activity was variable, LDH levels beginning to appear at the 7 th minute of ligation, declining in the first 2 hours post-ligation, and returning to normal 7 days after the onset of ischemia (reperfusion period).…”

Section: Discussionmentioning

confidence: 99%

Black sugarcane decoction reduces rat brain ischemia

Handayani

Nugraha

Nurmasitoh

et al. 2016

UnivMed

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Section: Discussionmentioning

confidence: 99%

Black sugarcane decoction reduces rat brain ischemia

Handayani

Nugraha

Nurmasitoh

et al. 2016

UnivMed

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“…However, few are able to elucidate the enzymatic process mechanistically. Known models include Michaelis–Menten (M–M) Equation, Hill Equation 13 – 16 , Binding Model of Alberty 17 , 18 , Monod–Wyman–Changeux (MWC) Model 11 , 19 , and empirical models derived from software of JMP 18 . The deficiency of these models is mainly the failure in taking a comprehensive consideration of the complex interactive processes and allosteric properties of the oligomeric enzyme.…”

Section: Introductionmentioning

confidence: 99%

A mechanistic kinetic description of lactate dehydrogenase elucidating cancer diagnosis and inhibitor evaluation

Tang

Oliveira

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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As a key enzyme for glycolysis, lactate dehydrogenase (LDH) remains as a topic of great interest in cancer study. Though a number of kinetic models have been applied to describe the dynamic behavior of LDH, few can reflect its actual mechanism, making it difficult to explain the observed substrate and competitor inhibitions at wide concentration ranges. A novel mechanistic kinetic model is developed based on the enzymatic processes and the interactive properties of LDH. Better kinetic simulation as well as new enzyme interactivity information and kinetic properties extracted from published articles via the novel model was presented. Case studies were presented to a comprehensive understanding of the effect of temperature, substrate, and inhibitor on LDH kinetic activities for promising application in cancer diagnosis, inhibitor evaluation, and adequate drug dosage prediction.

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Effect of Hypothermia on Kinetic Characteristics of Lactate Dehydrogenase in Rat Brain under Conditions of Global Ischemia and Reperfusion

2017

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We studied activity and kinetic characteristics of lactate dehydrogenase (LDH) in rat brain under conditions of incomplete global ischemia followed by reperfusion against the background of mild hypothermia. It was found that hypothermia leads to a decrease in LDH activity in the ischemic brain; the maximum velocity of the enzyme-catalyzed activity decreased and Michaelis constant increased, due to which the efficiency of catalysis decreased to the level observed in control rats. Ischemia against the background of hypothermia was accompanied by a decrease in the inhibition constant and narrowing of effective pyruvate concentration range. Blood flow resumption in the ischemic brain against the background of mild hypothermia led to an increase in LDH activity, the maximum reaction velocity increased, and Michaelis constant decreased, which lead to a significant increase in the efficiency of catalysis. This was accompanied by an increase in enzyme inhibition constant and a shift of the optimum on the concentration curve towards lower pyruvate concentrations.

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Analysis of the kinetic characteristics of lactate dehydrogenase from the rat brain during ischemia and reperfusion

Cited by 6 publications

References 28 publications

Black sugarcane decoction reduces rat brain ischemia

Black sugarcane decoction reduces rat brain ischemia

A mechanistic kinetic description of lactate dehydrogenase elucidating cancer diagnosis and inhibitor evaluation

Effect of Hypothermia on Kinetic Characteristics of Lactate Dehydrogenase in Rat Brain under Conditions of Global Ischemia and Reperfusion

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