Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE

Aksakal, Abdulbaki

doi:10.17221/2986-vetmed

Cited by 18 publications

(16 citation statements)

References 13 publications

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“…However, a different result was demonstrated when the technique is applied to a group of bacteria of the genus Salmonella (Aksakal, 2010). Ghazi et al (2009) states that the result of analysis of whole cellular protein profiles can be used to strengthen, complement and confirm the results of the phenotypic identification of lactic acid bacteria group.…”

Section: Resultsmentioning

confidence: 99%

“…SDSPAGE method can generate a complex pattern of protein bands and stable so that it can be used to interpret and compare the protein profiles of samples tested strain with the reference strain. Meanwhile, Aksakal (2010) concluded that whole cellular protein profiles cannot be used to distinguish between strains in the genus Salmonella. This study showed that the whole cellular protein profiles by SDSPAGE technique can be used as one method to identify the new strains of acetic acid bacteria, although it is not necessarily suitable to the other group of bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Cell protein extraction and electrophoresis were carried out by modification of the procedure developed by Scopes (1987) and Aksakal (2010). Four strains indigenous celluloseproducing acetic acid bacteria (ANG29, KRE65, ANG32 and SAL53) and two reference strains (G. xylinus 769 BTCC and G. hansenii NBRC 14820T) each was grown in 100 ml of HestrinSchramm (HS) medium and then incubated aerobically in a shaker incubator at 100 rpm, 30°C for 72 hours.…”

Section: Extraction Of Total Proteinsmentioning

confidence: 99%

“…The latest genus found is Granulibacter (Greenberg et al, 2006), which is the only member of the Acetobacteraceae family that is pathogenic. Sodium Dodecyl Sulphate Poliacrylamide Gel Electrophoresis (SDS PAGE) protein pattern analysis has been successfully used to identify various types of bacteria, including Listeria species (Park et al, 2006), lactic acid bacteria (Ghazi et al, 2009), Salmonella serovars (Aksakal, 2010), and to solve problems in the identification of closely related species in the genera Lactobacillus and Leuconostoc (Dicks et al, 1990; Pot et al, 1993; Kim et al, 2003; Sanchez et al, 2003; Jin et al, 2008. However, the usefulness of this method for the identification of cellulose producing acetic acid bacteria strains isolated from ripe fruits and nata inoculum has not yet been assessed.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

Sarkono

Moeljopawiro

Setiaji

et al. 2017

Indones. J. Biotechnol.

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This study was carried out to analyze the suitability of the identification of four indigenous cellulose producing acetic acid bacterial isolates (ANG29, KRE65, ANG32 and SAL53) based on the analysis of whole cellular protein profiles against identification based on phenotypic traits. Whole cellular protein profiles were determined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) method. The whole cellular protein profiles obtained from sample isolates, were compared with reference isolates for species identification. The results showed that based on visual observations can be determined as much as 12 bands of protein with a molecular weight of 19,099 KDa up to 132.182 KDa. Based on the analysis of protein bands were detected visually, fourth indigenous cellulose producing acetic acid bacterial isolates in the study had a higher similarity profile to the reference strain Gluconacetobacter xylinus BTCC 769 compared with other reference strains namely G. hansenii NBRC 14820 T . This condition is consistent with the results of the identification of fourth cellulose producing acetic acid bacterial isolates based on phenotypic traits. Thus, the whole cellular protein profiles by SDSPAGE technique can be used as a one of method to identification of cellulose producing acetic acid bacterial isolates.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Extraction Of Total Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

Sarkono

Moeljopawiro

Setiaji

et al. 2017

Indones. J. Biotechnol.

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“…Bacterial protein profiles are a reflection of its genome and therefore whole protein content plays a crucial role in comparative studies of bacteria [1]. Allicin kills bacteria through partial inhibition of DNA and protein synthesis and total inhibition of RNA synthesis [4].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Mechanism of Action of Crude Garlic (Allium sativum) Clove Extract on Selected Probiotic Bifidobacterium Species as Revealed by SEM, TEM, and SDS-PAGE Analysis

Booyens

Labuschagne

Thantsha

2013

Probiotics & Antimicro. Prot.

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There has been much research on the effects of garlic (Allium sativum) on numerous pathogens, but very few, if any, studies on its effect on beneficial, probiotic bifidobacteria. We have recently shown that garlic exhibits antibacterial activity against bifidobacteria. The mechanism by which garlic kills bifidobacteria is yet to be elucidated. This study sought to determine the mechanism of action of garlic clove extract on selected Bifidobacterium species using scanning and transmission electron microscopy and SDS-PAGE analysis. SEM micrographs revealed unusual morphological changes such as cell elongation, cocci-shaped cells with crosswalls and distorted cells with bulbous ends. With TEM observed changes included among others, condensation of cytoplasmic material, disintegration of membranes and loss of structural integrity. SDS-PAGE analysis did not reveal any differences in whole cell protein profiles of untreated and garlic clove extract treated cells. The current study is the first to reveal the mechanism of action of garlic clove extract on probiotic Bifidobacterium species. The results indicate that garlic affect these beneficial bacteria in a manner similar to that exhibited in pathogens. These results therefore further highlight that caution should be taken especially when using raw garlic and probiotic bifidobacteria simultaneously as viability of these bacteria could be reduced by allicin released upon crushing of garlic cloves, thereby limiting the health benefits that the consumer anticipate to gain from probiotics.1

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Subtractive Protein Profiling of Salmonella typhimurium Biofilm Treated with DMSO

2017

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Salmonella typhimurium is an important biofilm-forming bacteria. It is known to be resistant to a wide range of antimicrobials. The present study was carried out to evaluate the effects of dimethyl sulfoxide (DMSO) against S. typhimurium biofilm and investigate whole-cell protein expression by biofilm cells following treatment with DMSO. Antibiofilm activities were assessed using pellicle assay, crystal violet assay, colony-forming unit counting and extracellular polymeric substance (EPS) matrix assay whilst differential protein expression was investigated using a combination of one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, tandem mass spectrometry and bioinformatics. Treatment with 32% DMSO inhibited pellicle formation, biofilm viability, biofilm biomass and several important components of EPS matrix. Subtractive protein profiling identified two unique protein bands (25.4 and 51.2 kDa) which were present only in control biofilm and not in 32% DMSO-treated biofilm. In turn, 29 and 46 proteins were successfully identified from the protein bands of 25.4 and 51.2 kDa respectively. Protein interaction network analysis identified several biological pathways to be affected, including glycolysis, PhoP-PhoQ phosphorelay signalling and flagellar biosynthesis. The present study suggests that DMSO may inhibit multiple biological pathways to control biofilm formation.

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Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE

Cited by 18 publications

References 13 publications

Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

Analysis of Whole Cell Protein Profiles by SDS-PAGE to Identify Indigenous Cellulose-producer Acetic Acid Bacteria

In Vitro Antibacterial Mechanism of Action of Crude Garlic (Allium sativum) Clove Extract on Selected Probiotic Bifidobacterium Species as Revealed by SEM, TEM, and SDS-PAGE Analysis

Subtractive Protein Profiling of Salmonella typhimurium Biofilm Treated with DMSO

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