Analytical Application of a Sample Process Control in Detection of Foodborne Viruses

Diez‐Valcarce, Marta; Cook, Nigel; Hernández, Marta; Rodrı́guez-Lázaro, David

doi:10.1007/s12161-011-9262-9

Cited by 35 publications

(21 citation statements)

References 24 publications

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“…Quality control is therefore important in the pre-amplification analytical procedure. The use of a sample process control was reported for assessing the performance of extraction of target viruses from food products like strawberry, lettuce, and shellfish (Diez-Valcarce et al 2011). In this study, we processed food items according to the China Entry-Exit Inspection and Quarantine Industry Standards SNT 2626-2010.…”

Section: Discussionmentioning

confidence: 99%

An outbreak of multiple norovirus strains on a cruise ship in China, 2014

et al. 2015

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Section: Discussionmentioning

confidence: 99%

An outbreak of multiple norovirus strains on a cruise ship in China, 2014

et al. 2015

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“…Upon arrival, untreated wastewater samples were divided into 2 × 40 ml aliquots and stored at −20 °C before use. One aliquot was seeded with a known amount of murine NoV (MNV1), added to the samples as a sample process control (Diez-Valcarce et al 2011; D’Agostino et al 2011), in order to calculate viral recovery efficiency (by quantitative PCR) and check for potential inhibitors (by qualitative PCR). The PCR primers used in the study are shown in Table 2.…”

Section: Methodsmentioning

confidence: 99%

GIV Noroviruses in Wastewaters and in Stool Specimens from Hospitalized Patients

et al. 2013

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Noroviruses (NoVs) are important human pathogens associated with foodborne and waterborne gastroenteritis. These viruses are genetically highly heterogeneous, with more than forty genotypes within three genogroups (GI, GII, and GIV) identified in humans. However, the vast majority of human infections are associated with variants of a unique genotype, GII.4. Aside from these NoV strains of epidemiological relevance, NoV strains of genogroup GIV (Alphatron-like) are reported in a sporadic fashion and their overall prevalence in the community is unknown and this likely reflects the lack of specific diagnostic tools. We analyzed raw sewages collected from 32 wastewater treatment plants distributed throughout Italy (307 samples) and stool specimens collected from hospitalized patients with clinical signs of diarrhea of unknown etiology (285 samples). By using specific qualitative and quantitative RT-PCR assays, 21.8 % of the sewage samples and 3.2 % of the stool specimens tested positive for GIV NoVs. The number of genome copies in fecal samples ranged from 5.08 × 104 to 1.73× 106/g of feces. Sequence analysis showed limited genetic variability in human GIV viruses. The presence of GIV NoV both in sewage and in clinical samples confirms that not only GI and GII NoVs but also GIV strains are circulating in humans. Monitoring of GIV NoV is recommended in order to understand the dynamics of circulation in human populations, environmental contamination, and potential health risks.

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“…Nucleic acid extraction and rRT-PCR were performed according to standardized Integrated Monitoring and Control of Foodborne Viruses in European Food Supply Chains protocols. An extraction control virus, murine norovirus (MNoV), was placed in all samples before the lysis step of the extraction (8) to demonstrate the extraction of amplifi able nucleic acid. We performed all rRT-PCRs with an internal amplifi cation control (8).…”

Section: The Studymentioning

confidence: 99%

“…An extraction control virus, murine norovirus (MNoV), was placed in all samples before the lysis step of the extraction (8) to demonstrate the extraction of amplifi able nucleic acid. We performed all rRT-PCRs with an internal amplifi cation control (8).…”

Section: The Studymentioning

confidence: 99%

Hepatitis E Virus in Pork Food Chain, United Kingdom, 2009–2010

Berto¹,

Martelli²,

Grierson³

et al. 2012

Emerg. Infect. Dis.

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We investigated contamination by hepatitis E virus (HEV) in the pork production chain in the United Kingdom. We detected HEV in pig liver samples in a slaughterhouse, in surface samples from a processing plant, and in pork sausages and surface samples at point of sale. Our fi ndings provide evidence for possible foodborne transmission of HEV during pork production. D uring the past 10 years, hepatitis E virus (HEV) infection acquired in industrialized regions worldwide in the apparent absence of contaminated drinking water for fecaloral transmission has been reported (1). In Japan, foodborne transmission of HEV through ingestion of contaminated Sika deer, wild boar, and pig meat has been demonstrated (2) and, in France, after ingestion of pig liver sausage (3). Detection of HEV in pig liver sold in retail locations has been reported from Japan, the United States, and the Netherlands in 1.9%, 14.0%, and 6.5%, respectively (4,5). PCR indicated that 1 (1.3%) of 76 pig livers collected at retail outlets in southwestern England was positive for HEV (6).The European Commission Framework Program 7 project, Integrated Monitoring and Control of Foodborne Viruses in European Food Supply Chains, aimed to gather data on virus contamination of food and environmental sources for quantitative viral risk assessment and development of virus-specifi c guidance for food supply chain operators. The UK Animal Health and Veterinary Laboratories Agency investigated the pork food chain for HEV from slaughterhouse to point of sale. We investigated fecal contamination of pork and work surfaces during this study. The StudyDuring September 2009-October 2010, we collected samples from slaughtered pigs, human hands, and the environment at perceived critical points for virus contamination in a pig slaughterhouse, a processing plant, and 3 points of retail sale. Food safety fact-fi nding visits were made to the premises during which, through direct observations of conditions and practices, more points were identifi ed where contamination with viruses possibly could occur and from where ad hoc surface samples were collected by using sterile gauze swabs and placed in in phosphate-buffered saline plus antimicrobial drugs.We tested all samples collected by using real-time reverse transcription PCR (rRT-PCR) (7) for HEV. In addition, samples were tested for porcine adenovirus (PAdV) and human adenovirus by PCR as indicators of porcine and human fecal contamination, respectively. Nucleic acid extraction and rRT-PCR were performed according to standardized Integrated Monitoring and Control of Foodborne Viruses in European Food Supply Chains protocols. An extraction control virus, murine norovirus (MNoV), was placed in all samples before the lysis step of the extraction (8) to demonstrate the extraction of amplifi able nucleic acid. We performed all rRT-PCRs with an internal amplifi cation control (8).At the slaughterhouse, 40 carcasses were selected. Ten carcasses were randomly selected from each of 4 groups of pigs slaughtered that day; each group c...

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Analytical Application of a Sample Process Control in Detection of Foodborne Viruses

Cited by 35 publications

References 24 publications

An outbreak of multiple norovirus strains on a cruise ship in China, 2014

An outbreak of multiple norovirus strains on a cruise ship in China, 2014

GIV Noroviruses in Wastewaters and in Stool Specimens from Hospitalized Patients

Hepatitis E Virus in Pork Food Chain, United Kingdom, 2009–2010

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