Analytical studies of rapidly inactivating and noninactivating sodium currents in differentiated NG108-15 neuronal cells

Wu, Sheng‐Nan; Chen, Bing-Shuo; Hsu, Tai-I; Peng, Hsung; Wu, Yung-Han; Lo, Yi-Ching

doi:10.1016/j.jtbi.2009.05.003

Cited by 23 publications

(46 citation statements)

References 29 publications

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“…Interestingly, this figure is highly similar to the normalized I Na – I leak relationship of Figure 1C, suggesting that the I Na inhibition in the studies of Nesin and coworkers is largely, if not fully, due to I leak . Comparable relationships, but with a bend at a larger I leak , are found using less negative V 1/2 values, as found in other I Na studies on NG108‐15 cells [Lamas et al, 1995; Huang et al, 2008; Wu et al, 2009].…”

supporting

confidence: 74%

Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Verkerk

Ginneken

Wilders

2012

Bioelectromagnetics

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In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage-gated sodium current (I(Na)) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non-inactivating "leak" current (I(leak)), which was attributed to the formation of nanoelectropores or larger pores in the plasma membrane. We demonstrate that the increase in I(leak), in combination with a residual series resistance, leads to an error in the holding potential in the patch clamp experiments and an unanticipated inactivation of the sodium channels. We conclude that the observed inhibition of I(Na) may be largely, if not fully, artifactual.

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supporting

confidence: 74%

Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Verkerk

Ginneken

Wilders

2012

Bioelectromagnetics

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“…The I Na(NI) was previously demonstrated to be present in NG108-15 cells at our laboratory (Wu et al 2009a). In a separate series of experiments, investigations were further undertaken to evaluate whether EUG has any effects on the amplitude of I Na(NI) in response to long-lasting ramp pulse.…”

Section: Resultsmentioning

confidence: 84%

“…The parameter value of m ∞Na(NI) was originally taken from the paper of Wu et al (2009a). α m(Na) and β m(Na) are the forward and backward rate constants of activation variable (m) for I Na , respectively.…”

Section: Simulation Modelingmentioning

confidence: 99%

“…We recently characterized an I Na(NI) in NG108-15 cells (a somatic cell hybrid; mouse neuroblastoma and rat gliomas cells) differentiated with an analogue of cyclic adenosine monophosphate (Huang et al 2008;Wu et al 2009a). This neuronal cell line has been widely used as a neuron model in electrophysiology and pharmacology researches (Meves et al 1999;Wu et al 2001;Tsai et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Characterizing the effects of Eugenol on neuronal ionic currents and hyperexcitability

et al. 2011

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“…Wu, et al reported that the non-inactivating Na + current was characterized in differentiated NG108-15 cells, and this type of Na + current might facilitate neuronal hyper-excitability [37]. However, the origin of the non-inactivating Na + current is unclear.…”

Section: Discussionmentioning

confidence: 99%

Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells

Liu

Zhang

et al. 2012

BMC Neurosci

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BackgroundThe generation of action potential is required for stimulus-evoked neurotransmitter release in most neurons. Although various voltage-gated ion channels are involved in action potential production, the initiation of the action potential is mainly mediated by voltage-gated Na+ channels. In the present study, differentiation-induced changes of mRNA and protein expression of Na+ channels, Na+ currents, and cell membrane excitability were investigated in NG108-15 cells.ResultsWhole-cell patch-clamp results showed that differentiation (9 days) didn’t change cell membrane excitability, compared to undifferentiated state. But differentiation (21 days) induced the action potential generation in 45.5% of NG108-15 cells (25/55 cells). In 9-day-differentiated cells, Na+ currents were mildly increased, which was also found in 21-day differentiated cells without action potential. In 21-day differentiated cells with action potential, Na+ currents were significantly enhanced. Western blot data showed that the expression of Na+ channels was increased with differentiated-time dependent manner. Single-cell real-time PCR data demonstrated that the expression of Na+ channel mRNA was increased by 21 days of differentiation in NG108-15 cells. More importantly, the mRNA level of Na+ channels in cells with action potential was higher than that in cells without action potential.ConclusionDifferentiation induces expression of voltage-gated Na+ channels and action potential generation in NG108-15 cells. A high level of the Na+ channel density is required for differentiation-triggered action potential generation.

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Analytical studies of rapidly inactivating and noninactivating sodium currents in differentiated NG108-15 neuronal cells

Cited by 23 publications

References 29 publications

Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Sodium current inhibition by nanosecond pulsed electric field (nsPEF)—fact or artifact?

Characterizing the effects of Eugenol on neuronal ionic currents and hyperexcitability

Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells

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