Anastomosis groups and molecular variation in Pseudocercospora griseola

Abstract

ABSTRACT. The fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. 7437 Variation in Pseudocercospora griseola©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7436-7445 (2015) Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion, demonstrating the existence of genetic variability for loci involved in the control of this trait. High genotypic variability by RAPD markers was detected, but there was no relationship between genotype and anastomosis group. More studies are necessary to elucidate further the process of asexual recombination in this phytopathogen.

Pseudocercospora griseola, the causal agent of common bean angular leaf spot: Strain characterization and sensitivity to fungicides

“…Anastomoses between hyphae of P . griseola strains have been observed, which may also explain the variability observed in this fungus (Damasceno‐Silva et al, 2015).…”

“…This variability may be explained by several mechanisms, such as mutation, activity of transposable elements, intragenic recombination and genetic recombination due to the parasexual cycle of the strains. Anastomoses between hyphae of P. griseola strains have been observed, which may also explain the variability observed in this fungus(Damasceno-Silva et al, 2015). About 23% of the strains presented different levels of sensitivity to fungicides.…”