Angiotensin I-converting enzyme (ACE) activity of the tomato moth, Lacanobia oleracea: changes in levels of activity during development and after copulation suggest roles during metamorphosis and reproduction

Ekbote, Uma V.; Weaver, Robert J.; Isaac, R. Elwyn

doi:10.1016/s0965-1748(03)00105-x

Cited by 34 publications

(38 citation statements)

References 43 publications

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“…Activity peaked between pupal stages P6 and P8, and 20E increased the expression of an ACE-like gene in imaginal wing disc cells of B. mori (Quan et al, 2001). Ekbote et al (2003b) also reported that lepidopteran insects display an increase in ACE activity during metamorphosis. ACE activity increased approximately 4-fold during the last larval instar and early pupal stages of Lacanobia oleracea.…”

Section: Discussionmentioning

confidence: 91%

“…In Lacanobia oleracea, the highest level of ACE activity was found in the reproductive tract. Almost all of the enzyme was found in the accessory glands of the male and in the spermatheca and bursa copulatrix of the female (Ekbote et al, 2003b). ACE activity was also localized in the testis of N. bullata, Leptinotarsa decemlineata, and Locusta migratoria ).…”

Section: Discussionmentioning

confidence: 95%

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The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Vercruysse

Gelman

Raes

et al. 2004

Arch Insect Biochem Physiol

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The role of angiotensin converting enzyme (ACE, peptidyl dipeptidase A) in metamorphic- and reproductive-related events in the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae) was studied by using the selective ACE inhibitor captopril. Although oral administration of captopril had no effect on larval growth, topical administration to new pupae resulted in a large decrease of successful adult formation. Oviposition and overall appearance of adults emerging from treated larvae did not differ significantly from those emerging from non-treated larvae. In contrast, topical or oral administration of captopril to newly emerged adults caused a reduction in oviposition. By evaluating the effect of captopril on ecdysteroid titers and trypsin activity, we revealed an additional physiological role for ACE. Captopril exerted an inhibitory effect on ecdysteroid levels in female but not in male adults. Larvae fed a diet containing captopril exhibited increased trypsin activity. A similar captopril-induced increase in trypsin activity was observed in female adults. In male adults, however, captopril elicited reduced levels of trypsin activity. Our results suggest that captopril downregulates oviposition by two independent pathways, one through ecdysteroid biosynthesis regulation, and the other through regulation of trypsin activity. Apparently, fecundity is influenced by a complex interaction of ACE, trypsin activity, and ecdysteroid levels.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 95%

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Vercruysse

Gelman

Raes

et al. 2004

Arch Insect Biochem Physiol

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“…A similar transfer of AG ACE occurs in the tomato moth, Lacanobia oleracea, where the enzyme ends up in the female spermatheca and bursa copulatrix (Ekbote et al, 2003). It was not possible to follow the fate of the D. melanogaster AG ANCE, since the enzyme was also expressed in the female reproductive tissues (R. E. Isaac, unpublished).…”

Section: Discussionmentioning

confidence: 99%

Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid

Rylett

Walker

Howell

et al. 2007

Journal of Experimental Biology

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SUMMARY Angiotensin I-converting enzyme (ACE) expressed on the surface of endothelial cells is responsible for the last step in the synthesis of circulating angiotensin II and the inactivation of bradykinin. Mammalian ACE is also expressed in the prostate with other components of the renin–angiotensin system, and in developing spermatids, where the peptidase activity is known to be critical for normal sperm function. The importance of an ACE gene to male fertility has also been demonstrated in Drosophila melanogaster, where Ance is expressed in spermatids, and hypomorphic alleles of Ance cause a defect in spermiogenesis. Here we show that ANCE, which shares many enzymatic properties with mammalian ACE, is also a product of the male accessory gland of D. melanogaster. It is expressed in the secondary cells and is associated with the electron dense granule within the large vesicles of these cells. ACE proteolytic activity is lost from the accessory glands during mating,consistent with transfer to the mated female in the seminal fluid. The accessory gland ACE-like activity might have an evolutionarily conserved function processing biologically active peptides with a role in male fertility.

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“…It activates angiotensin I by converting it into the vasoconstrictive peptide angiotensin II and deactivates the vasodilator, bradykinin I, resulting in an increase in blood pressure (Erdö s and Skidgel, 1987). The prevalence of insect ACE in hemolymph (Macours et al, 2003;Ekbote et al, 2003, Lemeire et al, 2008, together with its broad in vitro substrate specificity suggests a role in the processing of neuropeptides and peptide hormones (Isaac et al, 2000). The first described insect ACE from the housefly Musca domestica hydrolyzes several C-terminally amidated neuropeptides like leucokinin I and II, locustatachykinin I and II, allatostatin I, SchistoFLRFamide, and Culex depolarizing peptides I and II (Lamango et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effect of ace inhibitors and TMOF on growth, development, and trypsin activity of larval Spodoptera littoralis

Lemeire

Borovsky

Camp

et al. 2008

Arch Insect Biochem Physiol

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Angiotensin converting enzyme (ACE) is a zinc metallopeptidase capable of cleaving dipeptide or dipeptideamide moieties at the C-terminal end of peptides. ACE is present in the hemolymph and reproductive tissues of insects. The presence of ACE in the hemolymph and its broad substrate specificity suggests an important role in processing of bioactive peptides. This study reports the effects of ACE inhibitors on larval growth in the cotton leafworm Spodoptera littoralis. Feeding ACE inhibitors ad lib decreased the growth rate, inhibited ACE activity in the larval hemolymph, and down-regulated trypsin activity in the larval gut. These results indicate that S. littoralis ACE may influence trypsin biosynthesis in the larval gut by interacting with a trypsin-modulating oostatic factor (TMOF). Injecting third instar larvae with a combination of Aea-TMOF and the ACE inhibitor captopril, down-regulated trypsin biosynthesis in the larval gut indicating that an Aea-TMOF gut receptor analogue could be present. Injecting captopril and enalapril into newly molted fifth instar larvae stopped larval feeding and decreased weight gain. Together, these results indicate that ACE inhibitors are efficacious in stunting larval growth and ACE plays an important role in larval growth and development.

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Angiotensin I-converting enzyme (ACE) activity of the tomato moth, Lacanobia oleracea: changes in levels of activity during development and after copulation suggest roles during metamorphosis and reproduction

Cited by 34 publications

References 43 publications

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid

Effect of ace inhibitors and TMOF on growth, development, and trypsin activity of larval Spodoptera littoralis

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