Anisotropic light scattering of individual sickle red blood cells

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

“…Recently, combined with a mathematical model, four important mechanical properties (bending modulus, shear modulus, area expansion modulus, and cytoplasmic viscosity) were simultaneously measured with QPI [80]. This method has been used to address the effects of osmotic pressure [83], malaria infection [78,86], and sickle cell disease [111,112]. …”

Measurement Techniques for Red Blood Cell Deformability: Recent Advances

“…FTLS showed significantly altered elastic and viscous membrane properties in sickle RBCs (Kim, Higgins et al,2012). Recently, four important m e c h a n i c a l p r o p e r t i e s o f s i c k l e R B C s w e re retrieved with memebrane fluctuations measurements (Byun, Higgins et al,under review).…”

“…By analyzing dynamic light scattering signals measured by FTLS, one can qualitatively access the membrane surface tension and viscosity of individual RBCs (Park, Diez-Silva et al,2010). Due to its capability of measuring light scattering signals from individual cells with high signal-to-noise ratio, FTLS has been employed to study several pathophysiological effects to the deformabiltiy of RBCs, including malaria infection (Park, Diez-Silva et al,2010), depletion of ATP (Park, Best-Popescu et al,2011), and sickle cell disease (Kim, Higgins et al,2012).…”

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

“…(2) Even though the particular focus of this study is on static 3-D images of phytoplankton, dynamics of 3-D RI tomograms of individual phytoplankton can allow to measure intracellular dynamics of subcellular organelles [39][40][41] or dynamics fluctuation in cell membrane [42][43][44][45][46][47] which can provide abundant information about pathophysiology of phytoplankton. (3) In addition, recent advances in QPI techniques can also be further employed to investigate phytoplankton research including super-resolution imaging [48], Fourier transform light scattering technique [49][50][51][52][53], real-time visualization of 3-D RI maps [54], multi-spectral QPI [55][56][57][58][59], and polarization-sensitive QPI [60,61]. (4) Furthermore, QPI techniques can be accessible to an existing microscope by attaching the recently developed QPI unit [62,63], which will further extend the applicability of QPI techniques for the study of phytoplankton.…”