Anti-biofim and Antibacterial Effect of Essential Oils and Their Major Compounds

Abstract

Essential oils are plant secondary metabolites commonly used in traditional medicine to treat infectious diseases. Along with their compounds, oils can contribute to development of new antimicrobial/antibiofilm products. Our study evaluated antibacterial activity of essential oils and their major compounds on Escherichia coli and Staphylococcus aureus planktonic cells and anti-biofilm activity. The effect of essential oils and their major compounds on biofilm and planktonic cells was assessed by quantifying the number of viable cells (CFU). Biomass quantification (absorbance = OD 570nm ) was also performed to evaluate anti-biofilm activity. Planktonic cells were more susceptible to the action of agents. Escherichia coli was reduced by 100 % with cinnamon and palmarosa oil. The treatment showed an interesting anti-biofilm activity, whereas green tea essential oil and its major compound, terpinen-4-ol, yielded less effective results. Reduction of viable cells in biofilm biomass was significant. Although our research is one of the first experiments in anti-biofilm activity of essential oils and their compounds against Escherichia coli and Staphylococcus aureus, pharmacological data confirm that the materials used in the trial do not pose health risk. Thus, essential oils and their compounds can be safely used in research to identify new antibacterial and anti-biofilm products against pathogenic bacteria.

Combination of essential oil and ciprofloxacin to inhibit/eradicate biofilms in multidrug-resistant Klebsiella pneumoniae

“…Hence, alternative strategies or effective agents to act against biofilm‐producing micro‐organisms are of great interest (Millezi et al . ). The discovery of anti‐infective agents which are active against planktonic micro‐organisms as well as microbial biofilms represents an ultimate objective (Ceylan et al .…”

“…During the last few years, the appearance of side effects of antibiotics has led to the search for new antimicrobial agents to overcome the foregoing disadvantage (Mohsenipour and Hassanshahian 2015). Hence, alternative strategies or effective agents to act against biofilm-producing micro-organisms are of great interest (Millezi et al 2016). The discovery of antiinfective agents which are active against planktonic micro-organisms as well as microbial biofilms represents an ultimate objective (Ceylan et al 2014).…”

Antimicrobial and antibiofilm activity of Cymbopogon flexuosus essential oil microemulsions

“…In some cases, the activity of the compound alone is not always more efficient than that of EO. In a study by Millezi et al (2016), EOs treatments of palmarosa, cinnamon, and green tea, as well as their respective major compounds, respectively, geraniol, eugenol, and terpine-4-ol, reduced planktonic and biofilm populations of S. aureus and E. coli, however, EOs proved to be more efficient. The activity of antibacterial substances varies according to some types of bacteria and arrangements.…”

“…In addition, the treatments with oils inoculated also in triplicate the positive control (0,0% of EO) containing the bacterial inoculum and the TSB broth (1:1 v/v), the negative control only EO and TSB medium (1:1 v/v) and white (only one TSB medium). Afterward, the microplate for the orbital was sent to the agitator (Tecnal, Brazil) and shaken at 37 ºC at 80 rpm for 24 h (Millezi et al, 2016). This procedure was also performed for analyses of Biomass Quantification and Quantification of Viable Cells after 24 h.…”

Antibacterial and anti-biofilm activity of cinnamon essential oil and eugenol

“…Biofilm formation in equipment and food processing surfaces causes several problems, including the corrosion of metal surfaces and crosscontamination of food products (MENON, 2016;DIAS et al, 2018). Among the pathogenic microorganisms S. aureus and E.coli are able to form biofilms, which are complex structures consisting of surface attached bacteria surrounded by a selfproduced extracellular polymer matrix (MILLEZI et al, 2016;FROZIET al., 2017).…”