Anti-inflammatory and anti-nociceptive activities of compounds from Tinospora sagittata (Oliv.) Gagnep.

Liu, Xiaohua; Hu, Zhenlin; Shi, Qiyun; Zeng, Hua‐Wu; Shen, Yun‐Heng; Jin, Hui‐Zi; Zhang, Wei‐Dong

doi:10.1007/s12272-010-0702-7

Cited by 47 publications

(30 citation statements)

References 17 publications

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“…The clear ROESY correlations (l " Fig. 3) for H-10/H-19, H-19/H-8 and H-8/H-12 indicated that H-10, H-19, and H-8 were on the same side of the molecular plane, and tentatively assuming an α-orientation according to the previously isolated diterpenoids from the Tinospora genus [2][3][4][16][17][18][19][20][21][22][23][24][25][26]. Finally, the structure of compound 1 was elucidated as shown in l " Fig.…”

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confidence: 67%

“…Its roots have widely been used for the treatment of sore throats, diarrhea, and superficial infections [1]. Previous investigations on the genus Tinospora showed that the clerodane diterpenes possess anti-inflammatory, antibacterial, and antifeedant activity [2][3][4][5][6]. In our recent study on the bioactivity of the Tinospora plants, the 95 % ethanolic extract of T. sagittata var.…”

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confidence: 99%

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New Clerodane Diterpenes from Tinospora sagittata var. yunnanensis

Jiang

Jiao

et al. 2014

Planta Med

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Four new clerodane diterpenes, namely sagittatayunnanosides A-D (1-4), were isolated from the roots of Tinospora sagittata var. yunnanensis, together with two known compounds, tinospinoside C (5) and tinospinoside E (6). The structures of the four new compounds were well elucidated by extensive analyses of the MS, IR, and 1D and 2D NMR data. The cytotoxic and antifouling activities of compounds 1-6 were evaluated.

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confidence: 67%

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confidence: 99%

New Clerodane Diterpenes from Tinospora sagittata var. yunnanensis

Jiang

Jiao

et al. 2014

Planta Med

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“…A voucher specimen (TSG2014) was deposited in the Veterinary Medicine Department of Sichuan Agricultural University. We prepared an extract of TSG from the plants according to reported methods [21]. Air-dried TSG (500 g) was refluxed with 95 % ethanol ( v/v ) three times each for 2 h. The solvent was completely removed in vacuo to generate a semisolid residue, which yielded 14.72 % ( w/w ) dry starting material.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo bactericidal activity of Tinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo and its main effective component, palmatine, against porcine Helicobacter pylori

Rong

Dong

et al. 2016

BMC Complement Altern Med

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BackgroundTinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo (TSG) is a traditional Chinese herb that has been used for the treatment of upper respiratory tract infection and has anti-bacterial and anti-ulcer activity. Our study investigated the bactericidal effects of TSG and its major component, palmatine, against a Helicobacter pylori (H. pylori) strain isolated from pig and the standard strain H. pylori SS1 in vitro and in vivo.MethodsH. pylori was isolated from pig and named H. pylori SCYA201401. For in vitro experiments, the inhibitory activity of TSG and palmatine against H. pylori SCYA201401 and H. pylori SS1 were tested by use of the agar cup diffusion technique. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined from the absence of H. pylori colonies on agar plates. Time-kill curves were used to evaluate bactericidal activity; the average number of colonies was calculated at 0 to 48 h after liquid incubation, with concentrations of drugs at 0.5, 1, and 2 × MIC. For in vivo experiments, H. pylori SCYA201401-infected mice were randomly divided into TSG, palmatine, triple therapy (omeprazole, clarithromycin, and amoxicillin), blank control, and model groups. The eradication ratios were determined by use of rapid urease tests and bacterial culture.ResultsIn vitro, the MIC and MBC of TSG against H. pylori SCYA201401 and SS1 were both 6250 μg/mL, whereas palmatine against H. pylori SCYA201401 was 6.25 μg/mL and against H. pylori SS1 was 3.12 μg/mL. The time-kill curves showed a dose-dependent, progressive decline in the numbers of viable bacteria up to 40 h. In vivo, the eradication ratios in the TSG and palmatine groups of mice were 80 and 50 % compared with 70 % in the triple-therapy group.ConclusionTSG and its major component, palmatine, have bactericidal activity against H. pylori in vitro and in vivo. The possibility that TSG or palmatine can be effective in the treatment of human and animals H. pylori infection deserves investigation.

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“…R. tinosporae is widely distributed in south of China, that is, Hunan, Guangdong, Guangxi, Guizhou, Sichuan, and Hubei provinces . In contemporary pharmacological study, it has been demonstrated that R. tinosporae has many biological activities, such as anti‐inflammatory , antibiosis, and antiviral effects . According to the literature, the major bioactive components in R. tinosporae can be divided into three types, including alkaloids, terpenoids, and sterols .…”

Section: Introductionmentioning

confidence: 99%

Analysis of six active components in Radix tinosporae by nonaqueous capillary electrophoresis with mass spectrometry

Liu

Zhou

Mao

et al. 2017

J of Separation Science

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Nonaqueous capillary electrophoresis with mass spectrometry has advantages for the analysis of active components in herbs. Here, a rapid nonaqueous capillary electrophoresis with mass spectrometry method was developed to separate, identify, and quantify palmatin, columbin, cepharanthine, menisperine, magnoflorine, and 20-hydroxyecdysone in Radix tinosporae. Electrospray ionization MS spectra of the six components were collected and possible cleavage pathways of main fragment ions were elucidated. The conditions that could affect separation, such as the composition of running buffer and applied voltage, were studied, and the conditions that could affect the mass spectrometry detection, such as the composition and flow rate of sheath liquid, the pressure of nitrogen gas, and the temperature and flow rate of the dry gas, were also optimized. Under the optimized conditions, the correlation coefficient was >0.99. The relative standard deviations of migration time and peak areas were <10%. The recoveries were calculated to be 99.31-107.80% in real samples. It has been demonstrated that the proposed method has good potential to be applied to determine the six bioactive components in Radix tinosporae.

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Anti-inflammatory and anti-nociceptive activities of compounds from Tinospora sagittata (Oliv.) Gagnep.

Cited by 47 publications

References 17 publications

New Clerodane Diterpenes from Tinospora sagittata var. yunnanensis

New Clerodane Diterpenes from Tinospora sagittata var. yunnanensis

In vitro and in vivo bactericidal activity of Tinospora sagittata (Oliv.) Gagnep. var. craveniana (S.Y.Hu) Lo and its main effective component, palmatine, against porcine Helicobacter pylori

Analysis of six active components in Radix tinosporae by nonaqueous capillary electrophoresis with mass spectrometry

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