Anti-inflammatory and antioxidant activities of constituents isolated from Pueraria lobata roots

Abstract

In order to evaluate the anti-inflammatory and antioxidant activities of Pueraria lobata roots and its active components, in vitro inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) protein expression, and tert-butylhydroperoxide (t-BHP)-induced reactive oxygen species (ROS) generation in RAW 264.7 cells, as well as in vitro scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), peroxynitrite (ONOO(-)), nitric oxide (NO·), superoxide anion (·O(2)(-)) and total ROS, and inhibitory activities against ONOO(-)-mediated tyrosine nitration, were determined. Repeated column chromatography was performed to isolate four known compounds from the anti-inflammatory and antioxidant EtOAc fraction: daidzein; genistein; puerarin; (+)-puerarol B-2-O-glucopyranoside; four known compounds from the anti-inflammatory n-hexane fraction: lupenone; lupeol; puerarol; coumestrol; seven known compounds from the antioxidant n-BuOH fraction: allantoin; 3'-hydroxypuerarin; daidzein 8-C-apiosyl-(1→6)-glucoside; puerarin; genistin; 3'-methoxypuerarin; daidzin. Among these compounds, lupenone and lupeol reduced NO production, as well as iNOS and COX-2 protein levels in LPS-stimulated RAW 264.7 cells. Furthermore, lupeol showed significant inhibitory activity against intracellular ROS generation by t-BHP. Meanwhile, 3'-hydroxypuerarin showed marked ONOO(-), NO·, total ROS scavenging activities, and weak ·O(2)(-) scavenging activity, while 3'-methoxypuerarin showed ONOO(-) scavenging activity and weak NO· and O(2)(-) scavenging activities, suggesting that existence of the 3'-hydroxyl group in puerarin plays an important role in the scavenging of ONOO(-), NO·, and total ROS, as well as inhibiting the ONOO(-)-mediated tyrosine nitration mechanism. These results indicate that P. lobata roots and its constituents may be a useful therapeutic and preventive approach to various inflammatory diseases and oxidative stress-related disease.

Extraction and purification of formonometin from <i>Trifolium pratense</i> L: Physicochemical properties of its complex with lecithin

“…Since isoflavones, one of the four compounds it contained can be used as a natural replacement of estrogen, it is popular for its function to alleviate climateric symptoms in older women. Formononetin (7-Hydroxy-4′-methoxyisoflavone, C 16 H 12 O 4 , CAS: 485-72-3, MW: 268.3), one of the major substance in Red clover, has become a focus of study due to its various pharmacological and biological activities, such as anti-tumor [3], anti-oxidant [4], anti-bacterial [5], anti-inflammatory [6], anti-atherosclerosis [7], and anti-osteoporosis [8]. Therefore, it is widely used as an additive in the food and pharmaceutical industries.…”

A polysaccharide isolated from Pueraria lobata enhances maturation of murine dendritic cells

“…Pueraria lobata is widely distributed in temperate regions of Korea, Japan, and China and is one of the earliest and most important edible crude herbs in traditional oriental medicine [14]. P. lobata has been used traditionally as an herbal medicine to treat the common cold, headache, diarrhea, and hypertension [15].…”

Anti-inflammatory effect of Seungmagalgeun-tang extract in human mast cells

“…승마 (Cimicifugae Rhizoma)는 甘辛하고 微寒해서 淸熱解毒, 發表透疹, 升陽擧陷하는 효능이 있다 17) . 갈근 (Puerariae Radix)은 甘辛하고 平해서 解肌透 熱, 發表透疹, 生津止渴, 升陽止瀉하는 효능이 있으며, 항산화, 항염증 효능이 밝혀져 있다 [17][18][19] . 작약 (Paeoniae Radix)은 苦酸하고 微寒해서 養血和營, 緩急止痛, 斂陰平肝의 효능이 있 으며 항염증 효능이 밝혀져 있다 17,20) .…”