Anti-inflammatory properties of mutolide isolated from the fungus Lepidosphaeria species (PM0651419)

Abstract

Mutolide an anti-inflammatory compound was isolated from the coprophilous fungus Lepidosphaeria sp. (PM0651419). The compound mitigated LPS-induced secretion of pro-inflammatory cytokines TNF-α and IL-6 from THP-1 cells as well as human peripheral blood mononuclear cells (hPBMCs). Mutolide also inhibited secretion of another pro-inflammatory cytokine IL-17 from anti-hCD3/anti-hCD28 stimulated hPBMCs. NF-κB is the major transcription factor involved in the secretion of pro-inflammatory cytokines including IL-17. Mechanistic evaluations revealed that mutolide inhibited induced NF-κB activation and translocation from cytoplasm into the nucleus. However, mutolide did not significantly affect activity of p38 MAPK enzyme, a serine/threonine kinase involved in cell cycle proliferation and cytokine secretion. These results indicate that mutolide may exert its anti-inflammatory effect via NF-κB inhibition. Oral administration of mutolide at 100 mg/kg showed significant inhibition of LPS-induced release of TNF-α from Balb/c mice in an acute model of inflammation. Our results highlight the anti-inflammatory properties of mutolide and suggest that further evaluation in a chronic model of inflammation is required to confirm the potential of mutolide as a druggable candidate for the treatment of inflammatory diseases.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1493-6) contains supplementary material, which is available to authorized users.

Evaluation of anti‐bacterial activity of silver nanoparticles synthesised by coprophilous fungus PM0651419

“…12 Iss. carry out the biotransformation at 0.5 mM of AgNO 3 , thus strengthening our belief on their biotransformation potential [35] The crystalline nature of biosynthesised AgNPs in this study using coprophilous PM0651419 fungus is comparable to reported literature on extracellular biosynthesis of AgNPs like the ones obtained from Fusarium oxysporum [54] and Penicillium brevicompactum [46]. Silver has been used since historical times as silver nitrate and silver sulfadiazine in the treatment of burns and infections [55].…”

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

“…The increasing resistance of pathogenic microorganisms to antibiotics in the world, and recently in Algeria, leading to the resurgence of more complex infections (Baba Ahmed-Kazi Tani and Arlet, 2014), has pushed researchers to discover new bioactive molecules. The capacity of endophytic fungi to biosynthesize bioactive secondary metabolites (more than 20.000 bioactive molecules) (Marson Ascêncio et al, 2014) and their various biological activities such as antimicrobial, anti-viral, anticancer, antioxidant, antidiabetic, and antiinflammatory activity (Sadrati et al, 2013;Bashyal et al, 2014;Shah et al, 2015;Jouda et al, 2016a;Pan et al, 2016;Singh and Kaur 2016;Yao et al, 2017) have made this fungus to be important, as an important source of new bioactive secondary metabolites potentially useful for human medicine and other domains.…”

Bioactive chromone constituents from <em>Vitex negundo</em> alleviate pain and inflammation

“… 27 – 29 Inflammation induced by carrageenan produces the cardinal features of inflammation and has been shown to mimic human clinical conditions. 30 , 31 Natural products possess clinical effectiveness in the prevention and treatment of inflammatory conditions 32 and their therapeutic prospective has been advocated to the marked anti-inflammatory properties in both in vivo animal models 33 , 34 and in vitro human cells’ 35 – 37 inflammation paradigms. Chromones exhibit putative anti-inflammatory properties.…”