Anti-yeast activity and characterisation of synthetic radish peptides Rs-AFP1 and Rs-AFP2 against food spoilage yeast

Shwaiki, Laila N.; Arendt, Elke K.; Lynch, Kieran M.

doi:10.1016/j.foodcont.2020.107178

Cited by 25 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 37 antifungal peptides isolated from Lactobacillus plantarum TE10 and their mixture can reduce A. flavus spore formation in fresh maize seeds (Muhialdin et al, 2020). Moreover, two chemically synthesized radish AMPs show a good inhibitory effect against different yeast species, such as Zygosaccharomyces bailii and Zygosaccharomyces rouxii (Shwaiki et al, 2020).…”

Section: Antifungal Peptides (Afps)mentioning

confidence: 99%

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

et al. 2020

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) are a class of small peptides that widely exist in nature and they are an important part of the innate immune system of different organisms. AMPs have a wide range of inhibitory effects against bacteria, fungi, parasites and viruses. The emergence of antibiotic-resistant microorganisms and the increasing of concerns about the use of antibiotics resulted in the development of AMPs, which have a good application prospect in medicine, food, animal husbandry, agriculture and aquaculture. This review introduces the progress of research on AMPs comprehensively and systematically, including their classification, mechanism of action, design methods, environmental factors affecting their activity, application status, prospects in various fields and problems to be solved. The research progress on antivirus peptides, especially anti-coronavirus (COVID-19) peptides, has been introduced given the COVID-19 pandemic worldwide in 2020.

show abstract

Section: Antifungal Peptides (Afps)mentioning

confidence: 99%

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Upon cooling from 80 °C back to 25 °C, the FTIR spectrum was similar to the one observed at 25 °C before heating, showing the reversible behavior of PsDef1 [ 10 ]. Furthermore, PgD5 defensin from P. glauca retained 71% of antifungal activity against Verticillium dahlia after 30 min of treatment at 75 °C and 61% at 100 °C [ 44 ], whereas rice defensin OsAFP1 demonstrated a negligible loss of activity after heating at 100 °C for 10 minutes [ 79 ], and defensins Rs-AFP1 and Rs-AFP2 were unaffected by exposure to 100 °C for 15 min [ 80 ]. Defensin-like antifungal peptide NRBAP from Phaseolus vulgaris is reported to retain activity following the exposure to 100 °C for 30 min and to extreme pH values, ranging from 0–1 (obtained by dissolving the NRBAP in hydrochloric acid) to 12 [ 81 ].…”

Section: Secondary and Tertiary Structuresmentioning

confidence: 99%

Plant Defensins from a Structural Perspective

Kovaleva

Bukhteeva

Kit

et al. 2020

IJMS

View full text Add to dashboard Cite

Plant defensins form a family of proteins with a broad spectrum of protective activities against fungi, bacteria, and insects. Furthermore, some plant defensins have revealed anticancer activity. In general, plant defensins are non-toxic to plant and mammalian cells, and interest in using them for biotechnological and medicinal purposes is growing. Recent studies provided significant insights into the mechanisms of action of plant defensins. In this review, we focus on structural and dynamics aspects and discuss structure-dynamics-function relations of plant defensins.

show abstract

“…The MIC of D-lp1 against the 5 yeast strains was also examined as described by Shwaiki, Arendt & Lynch, 2020 [ 44 ]. The assay was performed in compliance with the method outlined by the National Committee for Clinical Laboratory Standards (NCCLS M27A- [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

“…The fungistatic and fungicidal activity of the peptide against the susceptible yeast was also examined to determine its MFC as described by Shwaiki, Arendt and Lynch, 2020 [ 44 ]. One hundred microlitres of each yeast suspension from an antiyeast assay was spotted onto SD agar plates and incubated for 48−72 h, depending on the yeast.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Study on the Inhibitory Activity of a Synthetic Defensin Derived from Barley Endosperm against Common Food Spoilage Yeast

2020

Self Cite

View full text Add to dashboard Cite

In the food industry, food spoilage is a real issue that can lead to a significant amount of waste. Although current preservation techniques are being applied to reduce the occurrence of spoilage microorganisms, the problem persists. Food spoilage yeast are part of this dilemma, with common spoilers such as Zygosaccharomyces, Kluyveromyces, Debaryomyces and Saccharomyces frequently encountered. Antimicrobial peptides derived from plants have risen in popularity due to their ability to reduce spoilage. This study examines the potential application of a synthetic defensin peptide derived from barley endosperm. Its inhibitory effect against common spoilage yeasts, its mechanisms of action (membrane permeabilisation and overproduction of reactive oxygen species), and its stability in different conditions were characterised. The safety of the peptide was evaluated through a haemolysis and cytotoxicity assay, and no adverse effects were found. Both assays were performed to understand the effect of the peptide if it were to be consumed. Its ability to be degraded by a digestive enzyme was also examined for its safety. Finally, the peptide was successfully applied to different beverages and maintained the same inhibitory effects in apple juice as was observed in the antiyeast assays, providing further support for its application in food preservation.

show abstract

Anti-yeast activity and characterisation of synthetic radish peptides Rs-AFP1 and Rs-AFP2 against food spoilage yeast

Cited by 25 publications

References 51 publications

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

Plant Defensins from a Structural Perspective

Study on the Inhibitory Activity of a Synthetic Defensin Derived from Barley Endosperm against Common Food Spoilage Yeast

Contact Info

Product

Resources

About