Antibacterial Activity of Siwak (Salvadora persica Linn.) against Streptococcus mutans (ATC31987) and Bacteroides melaninogenicus

Zaenab, Zaenab; Mardiastuti, Mardiastuti H. W.; Anny, V. P.; Logawa, Benny

doi:10.7454/msk.v8i2.287

Cited by 8 publications

(9 citation statements)

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“…[13], each well filled with 150 μļ brain heart infusion liquid as a medium. Then, the extract of Laban leaves is taken with a concentration of 3.13% as much as 150 μļ and put into the first well on rows 1, 2, 5, and 6 and series dilutions were carried out, the results of dilution were 3.13%, 1.56%, 0.78%, 0.39%, 0.20%, 0.10%, and 0.05% [14]. Then, in all rows, wells 5, 6, 7, and 8 put a suspension S. mutans bacteria, each as much as 10 μ [13].…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity Tests of N-hexane, Ethyl Acetate, and Methanol Leaves (Vitex) Extract (pinnata) against Streptococcus mutans

Nuraskin

Marlina

Idroes

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Vitex pinnata is known as Laban, which is a medicinal plant used traditionally for generations to generations. Laban leaf extract with various concentrations has antibacterial activity. Laban leaf extract is known to inhibit the formation of Streptococcus mutant in human teeth. AIM: To exam, the minimum inhibitory concentration (MIC) and minimum kill concentration (KBM) extract Laban leaves (V. pinnata) as an antibacterial against Streptococcus mutants. METHODS: This research was purely experimental research with design randomized pretest-posttest and control group. The study was conducted at the Laboratory Microbiology Faculty of Animal Health, Syiah Kuala University, Banda Aceh, from March to June 2019. The sample of this study was the Laban leaf from the Aceh Besar geothermal area. This research included preparation raw materials, chemical characterization of raw materials, determination of minimum and maximum components mixture, determining the optimum formula based on the best inhibitory potential, inhibitory testing antibacterial, standardized test, and test (one-way ANOVA). RESULTS: MIC of n-hexane extract showed the results of calculations; the percentage of bacterial inhibition was at a MIC of 1.56% on average colony −1.45 CFU/ml. In ethyl acetate extract, the MIC was 0.20% on the average colony −0.17 CFU/ml. The methanol extract can inhibit bacteria at the smallest concentration of 0.05% average colony −1.48 CFU/ml. Methanol extract inhibits bacteria more quickly. Concentration results minimum kill was 1.56%, 0.78%, 0.39%, 0.20%, 0.10%, and 0.05%. The smallest concentration of extract (n-hexane, ethyl acetate, and methanol) can kill Streptococcus mutans bacteria that are marked in the absence of bacterial colonies on microbiological growth media. CONCLUSION: Extracts of n-hexane, ethyl acetate, and methanol from Laban leaves have inhibitory activity on the growth of S. mutans bacteria. The smallest concentration of extract (n-hexane, ethyl acetate, and methanol) is able to kill S. mutans bacteria.

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Section: Methodsmentioning

confidence: 99%

Antibacterial Activity Tests of N-hexane, Ethyl Acetate, and Methanol Leaves (Vitex) Extract (pinnata) against Streptococcus mutans

Nuraskin

Marlina

Idroes

et al. 2020

Open Access Maced J Med Sci

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“…10 Sementara siwak (Salvadora persica) mengandung bahan antiseptik, asam tanat, dan minyak atsiri yang dapat meningkatkan kesehatan mulut dengan memberikan efek antibakteri dan mencegah penurunan pH saliva. 11 Minyak atsiri daun cengkeh (Syzygium aromaticum L.) memiliki aktivitas antibakteri dan antibiofilm. Kandungan aktif yang berperan sebagai antibakteri pada minyak atsiri cengkeh adalah senyawa eugenol.…”

Section: Pendahuluanunclassified

Perbedaan Daya Hambat Pasta Gigi Berbahan Herbal Terhadap Pertumbuhan Streptococcus Mutans

Susi¹,

Bachtiar²,

Sali³

2015

MKA

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AbstrakKaries gigi dan penyakit periodontal dapat dicegah dengan mengontrol pembentukan plak secara teratur. Penggunaan pasta gigi herbal dapat memberikan efek kimia untuk mengontrol pembentukan plak. Studi terdahulu mendapatkan bahwa pasta gigi herbal dapat mengurangi jumlah bakteri utama pada rongga mulut yaitu Streptococcus mutans. Penelitian ini bertujuan untuk meneliti perbedaan daya hambat beberapa pasta gigi herbal (mengandung siwak, cengkeh, dan daun sirih) terhadap pertumbuhan Streptococcus mutans. Metode penelitian adalah eksperimental dengan meletakkan cakram yang sudah direndam dengan pasta gigi ke medium agar darah yang mengandung koloni Streptococcus mutans. Uji daya hambat bakteri dilakukan dengan metode difusi. Terbentuknya zona bening di sekitar koloni bakteri menunjukkan adanya penghambatan pertumbuhan bakteri uji. Hasil uji satu arah ANOVA menunjukkan adanya perbedaan daya hambat yang bermakna antar pasta gigi herbal yang digunakan (p<0.05). Ketiga pasta gigi didapakan memiliki kemampuan antibakteri kuat dengan rata-rata zona hambat 16.075 mm, 13.375 mm dan 11.080 mm. Jadi dapat disimpulkan bahwa pasta gigi herbal mempunyai efek anti bakteri terhadap pertumbuhan Streptococcus mutans dengan efek anti bakteri terkuat di tunjukkan oleh pasta gigi mengandung cengkeh. Kata kunci: Pasta gigi herbal, zona inhibisi, Streptococcus mutans. (siwak-, cloves-, and betel leavescontained) Abstract Dental caries and periodontal disease can be eliminated by regularly control plaque formation. The usage of herbal toothpaste is able to give chemical effect toward plaque control. Previous studies shown that the usage of herbal toothpaste was able to reduce the growth of Streptococcus mutans, the main bacteria in the mouth. The study aimed at investigating the difference of zone of inhibition of several herbal toothpastes

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“…4 Beberapa peneliti melaporkan adanya efek antibakteri dari siwak terhadap bakteri kariogenik dan pathogen periodontal khususnya spesies Bacterioides serta menghambat pembentukan plak. 5,6 Penelitian yang lain menyatakan bahwa ekstrak siwak memiliki daya antibakteri terhadap streptococcus mutans dan s.faecalis. 7 Aktifitas siwak dalam menurunkan pertumbuhan bakteri s.mutans disebabkan karena kandungan SCNyang ketika bereaksi dengan kelompok sulfhidril dalam enzim bakteri akan menyebabkan kematian bakteri, 8 sehingga produk asam tidak terbentuk dan pH plak tidak mengalami penurunan.…”

Section: Pendahuluanunclassified

“…17 Menurut World Health Organization Report Series, siwak dapat menghilangkan plak tanpa menyebabkan luka pada gigi. 5 Peneliti menyarankan untuk dilakukan penelitian lebih lanjut tentang pemakaian pasta gigi yang mengandung siwak dibandingkan pasta gigi siwak terhadap kesehatan rongga mulut dalam waktu yang lebih lama dan dengan metode yang lebih teliti. Dapat disimpulkan bahwa siwak dan pasta gigi siwak memiliki efek yang sama dalam menurunkan akumulasi plak pada anak.…”

Section: Hasilunclassified

Efektifitas siwak (Salvadora persica) dan pasta gigi siwak terhadap akumulasi plak gigi pada anak-anak (Effectiveness of Siwak (Salvadora persica) and siwak toothpaste on dental plaque accumulation in children)

Bramanti

Ula

et al. 2014

Dent. J. (Majalah Kedokteran Gigi)

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Antibacterial Activity of Siwak (Salvadora persica Linn.) against Streptococcus mutans (ATC31987) and Bacteroides melaninogenicus

Cited by 8 publications

References 0 publications

Antibacterial Activity Tests of N-hexane, Ethyl Acetate, and Methanol Leaves (Vitex) Extract (pinnata) against Streptococcus mutans

Antibacterial Activity Tests of N-hexane, Ethyl Acetate, and Methanol Leaves (Vitex) Extract (pinnata) against Streptococcus mutans

Perbedaan Daya Hambat Pasta Gigi Berbahan Herbal Terhadap Pertumbuhan Streptococcus Mutans

Efektifitas siwak (Salvadora persica) dan pasta gigi siwak terhadap akumulasi plak gigi pada anak-anak (Effectiveness of Siwak (Salvadora persica) and siwak toothpaste on dental plaque accumulation in children)

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