Antibacterial Effects of Green Betel (Piper betle Linn.) Leaf Against Streptococcus agalactiae and Escherichia coli

Abstract

This study investigated the effect of betel (Piper betle L.) leaf at two physiological ages (young, 3 rd leaf, and old, 8 th leaf) at three concentrations of crude boiled-water extracts (10%, 20%, and 30%) against Streptococcus agalactiae and Escherichia coli. A completely randomized design with nesting was adopted for the six treatments and six replicates. The inhibition zone on nutrient agar media was measured. Overall there were no significantly differences. However, the inhibition zone diameters with 10%, 20%, and 30% crude water extracts of young leaves with S. agalactiae were 5.18 ± 0.99, 5.96 ± 0.34, and 6.38 ± 1.52 mm, respectively, and for old leaves, they were 5.76 ± 0.62, 5.67 ± 0.43, and 4.68 ± 0.33 mm, respectively. For E. coli, the corresponding values for young leaves were 3.96 ± 1.45, 4.54 ± 0.72, and 3.51 ± 0.48 mm, respectively, and for old leaves, the values were 3.85 ± 1.20, 4.14 ±0.68, and 2.35 ± 0.83 mm, respectively. The strongest inhibition was shown by the 30% crude water extract of young leaves against S. agalactiae.

Literature Review : Manfaat dan Bioaktivitas Daun Sirih (Piper Betle L.) Sebagai Antibakteri

“…dalam ekstrak daun sirih hijau, misalnya bethel phenol. Sedangkan lipoprotein, membran luar, dan lipopolisakarida merupakan tiga polimer pembungkus pada bakteri gram negatif yang berada di luar lapisan peptidoglikan (8,22).…”

Betelvine (Piper betleL.): A comprehensive insight into its ethnopharmacology, phytochemistry, and pharmacological, biomedical and therapeutic attributes

“…agalactiae . 176 A recent experiment compared antibacterial efficacy between acetone extract and ethanol extract from leaves (Barguna and Moheshkhali) against the varieties of S . aureus , E .…”

“…coli and found that both extracts showed antibacterial activity, while 30% of the young leaf extract showed the highest activity against S . agalactiae 176 . A recent experiment compared antibacterial efficacy between acetone extract and ethanol extract from leaves (Barguna and Moheshkhali) against the varieties of S .…”

“…1 Surjowardojo et al tested the crude water extract of young and mature leaves in St. agalactiae and E. coli and found that both extracts showed antibacterial activity, while 30% of the young leaf extract showed the highest activity against S. agalactiae. 176 A recent experiment compared antibacterial efficacy between acetone extract and ethanol extract from leaves (Barguna and Moheshkhali) against the varieties of S. aureus, E. coli and B. cereus. The Barguna showed a MIC (minimum inhibition concentration) value of about 2.12 to 4.25 mg/ml, and the Moheshkhali variety showed a MIC of 2.12 to 8.5 mg/ml.…”

Pemberdayaan Ibu Rumah Tangga Melalui Pembuatan Handsanitizer Dalam Upaya Pencegahan Covid-19

“…Tanaman sirih (Piper betle Linn) merupakan jenis tanaman yang diketahui sangat berkhasiat sebagai antiseptik yang baik (Surjowardojo, Setyowati and Ambarwati, 2019;Erni et al, 2020). Bagian tanaman yang digunakan yaitu bagian daun karena banyak mengandung senyawa turunan fenol (Erni et al, 2020 Selama pelaksanaan kegiatan selalu dilakukan evaluasi dengan menggunakan metode diskusi untuk menilai pemahaman mitra tentang pengetahuan dalam mencegah penyebaran COVID-19 dan cara membuat hand sanitizer dari bahan alami daun sirih.…”