Antibacterial potential of Ni-doped zinc oxide nanostructure: comparatively more effective against Gram-negative bacteria including multi-drug resistant strains

Abstract

Synthesis of Ni2+-doped ZnO nanoparticles and their antibacterial activity.

Easy One-Pot Low-Temperature Synthesized Ag-ZnO Nanoparticles and Their Activity Against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

“…The bacterial cell wall is generally negatively charged (Ghosh et al, 2012), which enables electrostatic interaction with the Zn 2+ and Ag + present in AZO NPs (which were identified in our ZO and AZO NPs by TEM analysis and XPS). Disruption of the bacterial cell membrane by Ag-ZnO NPs can be another potential mechanism for antibacterial activity (Naskar et al, 2020), which we have corroborated here using SEM. Membrane damage generally results in increased inhibition of DNA/plasmid replication by Zn 2+ /Ag + ions and the production of proteins/enzymes that affect bacterial cell functioning and contribute to cell death.…”

“…Generally, antibacterial activity was evaluated according to a previous report (Naskar et al, 2020) using BBL TM Mueller-Hinton Broth (MHB, Becton Dickinson) grown bacterial strains including E. coli (ATCC 25922), A. baumannii (ATCC 19606), S. aureus (ATCC 25923); AMR strains of E. coli (1368), A. baumannii (12001); and different MRSA clinical isolates (Shin et al, 2019). Briefly, the MHB medium was used for the inoculation of single colonies of bacteria, which were incubated at 37 • C overnight, followed by dilution of the cells to an optical density of 0.5 McFarland turbidity standard using Sensititre TM Nephelometer (Thermo Scientific).…”

“…AMR is easily recognized in hard-to-treat pathogens and has become an alarming issue complicating health care and many other sectors (Eliopoulos et al, 2003;Jasovsky et al, 2016). For instance, methicillin-resistant Staphylococcus aureus (MRSA) is one of the most well-known AMR bacterial species for which immediate intervention is necessary, but even the long considered last-resort antibiotic vancomycin cannot be used in the treatment of MRSA infections since vancomycin-resistant S. aureus (VRSA) strains have emerged (Naskar and Kim, 2019;Naskar et al, 2020). In addition, AMR S. aureus species are one of 12 families of priority pathogenic bacteria listed by the World Health Organization (WHO) for which antibiotics are urgently needed (World Health Organization [WHO], 2017).…”

A New Nano-Platform of Erythromycin Combined with Ag Nano-Particle ZnO Nano-Structure against Methicillin-Resistant Staphylococcus aureus

“…Notably, bacteria can reduce their interactions with antibiotics to result in drug resistance [22]. However, the antibacterial mechanism of NPs [6,7] or, in this case, AZO NPs mainly involves membrane disruption of bacteria which occurs independently of direct interactions with bacterial components. Therefore, AZO NP may overcome resistance to antibiotics.…”

“…Because of their antibacterial activities, various metal and metal oxide nano-particles such as ZnO, CuO, TiO 2 , SiO 2 , MgO, Cao, Ag, Au, and Cu offer effective solutions for treating infections by MDR pathogens [5]. ZnO has been widely used by researchers because of its antibacterial activity and distinctive physiochemical properties such as high surface area-to-volume ratio [6,7]. The Food and Drug Administration has already recognized ZnO as a "GRAS" (generally recognized as safe) substance (FDA, 21CFR182.8991, USA) [8].…”