Antifungal dynamics of LY 303366, an investigational echinocandin B analog, against Candida ssp.

Ernst, Michael E.; Klepser, Michael E.; Wolfe, Erika J.; Pfaller, Michael A.

doi:10.1016/s0732-8893(96)00202-7

Cited by 66 publications

(59 citation statements)

References 3 publications

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“…Although RPMI 1640 broth medium was used in these timekill studies in an effort to parallel M27-A2, it is clear that the fungicidal activity of the echinocandins is significantly influenced by the choice of growth medium (16)(17)(18)(19). Some agents are not uniformly fungicidal against Candida spp.…”

Section: Efforts At Developing Standardized Methods For Performing Timentioning

confidence: 99%

Determination of Fungicidal Activities against Yeasts and Molds: Lessons Learned from Bactericidal Testing and the Need for Standardization

2004

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In certain unique clinical settings, the ability of the antimicrobial agent administered to kill the pathogen outright may be quite important. These situations invariably involve infection of a site not easily accessed by host defenses and/or of a structure with essential anatomic or physiologic function such as the heart (endocarditis), central nervous system (meningitis), or bone (osteomyelitis). Likewise, infections in immunosuppressed hosts, especially those who are neutropenic, are often thought to require microbicidal therapy. Proof of the cidal nature of an antimicrobial agent in vitro is tedious, complex, and fraught with error. Although several methods for assessing in vitro bactericidal activity have been standardized (NCCLS M26-A and M21-A), the clinical relevance of these determinations is questionable and the tests are performed infrequently in most laboratories. Most of the clinical data supporting the need for microbicidal therapy and testing have focused on bacterial infections. However, given the fact that most serious fungal infections occur in profoundly immunosuppressed individuals, it is generally assumed that a cidal regimen would be preferable in that setting as well. In view of this clinical concern and the perceived need to assess the fungicidal activity of a variety of agents, we considered that it would be useful to review what is known about the issues and problems in assessing bactericidal activity and the clinical utility of such measurements. Following this review, we discuss the issue of how one defines fungicidal activity in vitro and in vivo and how feasible it might be to determine the fungicidal activity of organism-drug combinations for purposes of both drug development and clinical care. Proposed methods for fungal time-kill determinations and minimal fungicidal concentration determinations are also discussed

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Section: Efforts At Developing Standardized Methods For Performing Timentioning

confidence: 99%

Determination of Fungicidal Activities against Yeasts and Molds: Lessons Learned from Bactericidal Testing and the Need for Standardization

2004

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“…After 24-48 hours of incubation at 35±2°C, a reading was made to evaluate the MFC, as based on the growth of the controls. The MFC was defined as the lowest product concentration that inhibited growth of the yeast or permitted less than three CFUs to occur, resulting thus in 99.9% fungicidal activity (ERNST et al, 1996, ESPINEL-INGROFF, 2002.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning

confidence: 99%

Study of the antifungal potential of (r)-(+)-citronellal and its association with therapeutic agents used in the treatment of vulvovaginal candidiasis

Medeiros¹,

Silva²,

Pérez³

et al. 2017

Biosci. J.

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ABSTRACT:Vulvovaginal candidiasis (VVC) is a common fungal infection that affects healthy women of all ages. At least 75% of women will develop one or more infections once during their lifetime, with 6 to 9% of those individuals developing recurrent infections. In view of this context, this study sought to evaluate the antifungal potential of the isolated (R)-(+)-citronellal [(R)-(+)-CT] and associated to therapeutic agents of clinical importance. The enantiomer was solubilized in tween 80 and dimethylsulfoxide (DMSO). Posteriorly diluted in sterile distilled water up to the concentration of 2048µg/mL. The minimum inhibitory concentration (MIC) of the product was determined by microdilution in RPMI-1640 obtaining dilutions of 1024-4µg/mL. The minimum fungicidal concentration (MFC) was determined by the Sabouraud dextrose agar (SDA) depletion technique from aliquots of 1µL of the MIC, MIC × 2 and MIC × 4. The MIC and the MFC values of (R)-(+)-CT for 90% of the C. albicans strains were 16 and 32µg/mL respectively. In the susceptibility test, C. albicans presented a high resistance to fluconazole and to itraconazole, 12 (92.30%) of the strains. However, for ketoconazole and miconazole the resistance was of 4 (30.76%) and 3 (23.07%) of the strains respectively. In the combination testing of the (R)-(+)-CT with ketoconazole and miconazole, the resistance was completely reverted. For fluconazole and itraconazole, the resistance was reverted in 9 (75%) and 7 (58.33%) of the strains respectively. The (R)-(+)-CT presented fungicide activity with MFC of MIC × 2. When in combination with ketoconazole, fluconazole, itraconazole and miconazole increased the inhibition zones of these antifungal drugs, reducing the resistance against C. albicans.

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“…Other components with significant concentrations were 1-felandreno (16.70%), p-cymene (10.21%), sabinene (8.14%) and trans-caryophyllene (4.81%). Although there are known no studies to date on the chemical composition of C. rhamnifolioides essential oil, the compounds identified in this study are consistent with the data for Croton spp., with the essential oils being characterised by a predominance of monoterpenes and sesquiterpenes (MECCIA et al, 2000;FONTENELLE et al, 2008).…”

Section: Chemical Compositionmentioning

confidence: 99%

“…There was a bactericidal effect of the essential oil at a concentration of 20 μL.mL -1 against A. hydrophila, with the reduction of four logarithmic cycles after a 1-h exposure (Figure 1). A bactericidal effect against S. aureus and E. coli was evident after 6 h at concentrations of 10 and 20 μL.mL -1 , respectively, and against L. monocytogenes after 8 h. Some researchers have proposed that a compound be recognised as a strong bactericide when it can cause a decrease of 1000 times (three logarithmic cycles or 99.9%) relative to the initial inoculum (ERNST et al, 1996;LAPLANTE, 2007).…”

Section: Effect Of Oil On the Viable Cell Countmentioning

confidence: 99%

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

et al. 2013

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This study describes the chemical composition and antimicrobial activity of an essential oil from the leaves of Croton rhamnifolioides Pax&Hoffm., an aromatic plant widely distributed in northeastern Brazil and commonly used in folk medicine. Eight components were identified: 1.8-cineole (46.32%) as the major compound, followed by 1-felandreno (16.70%), p-cymene (10.21%), sabinene (8.14%) and trans-caryophyllene (4.81%). The essential oil exhibited antibacterial activity against Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis and Staphylococcus aureus, with minimum inhibitory concentrations and minimum bactericidal concentrations ranging from 2.5 to 20 and 5 to 40 µL.mL -1 , respectively. In addition, the essential oil also affected the cellular viability of these bacteria. The results suggest the need for research to establish the potential uses of this essential oil as a natural antimicrobial in the food industry. Key words: Croton rhamnifolioides, essential oil, antimicrobial activity, chemical composition ResumoO presente estudo descreve a composição química e a atividade antimicrobiana do óleo essencial das folhas de Croton rhamnifolioides Pax & Hoffm, uma planta aromática amplamente distribuída no Nordeste do Brasil e largamente utilizada na medicina popular. Cromatografia gasosa -espectrometria de massa foi utilizada para determinar sua composição. Oito componentes foram identificados, sendo 1,8-cineol (46,32%) o composto majoritário, seguido por 1-felandreno (16,70%), p-cimeno (10,21%), sabineno (8,14%) e trans-cariofileno (4,81%). O óleo essencial apresentou atividade antibacteriana contra Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis e Staphylococcus aureus com valores de concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM) entre 2,5 -20 μL/mL e 5 -40 μL/mL, respectivamente. Além disso, o óleo essencial também afetou a viabilidade celular dessas bactérias. Os resultados sugerem a necessidade de estudos para estabelecer o uso potencial deste óleo essencial como antimicrobiano natural na indústria de alimentos. Palavras-chave: Croton rhamnifolioides, óleo essencial, atividade antimicrobiana, composição química

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Antifungal dynamics of LY 303366, an investigational echinocandin B analog, against Candida ssp.

Cited by 66 publications

References 3 publications

Determination of Fungicidal Activities against Yeasts and Molds: Lessons Learned from Bactericidal Testing and the Need for Standardization

Determination of Fungicidal Activities against Yeasts and Molds: Lessons Learned from Bactericidal Testing and the Need for Standardization

Study of the antifungal potential of (r)-(+)-citronellal and its association with therapeutic agents used in the treatment of vulvovaginal candidiasis

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

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