Antimicrobial activity of the volatile compound 3,5‐dichloro‐4‐methoxybenzaldehyde, produced by the mushroom Porostereum spadiceum , against plant‐pathogenic bacteria and fungi

Abstract

Aims: In this study, volatile compounds released from mycelia of some aromatic mushrooms were investigated for their inhibitory activity against plant-pathogenic bacteria and fungi. Methods and Results: A screening revealed that volatile compounds from mycelia of Porostereum spadiceum remarkably inhibited the colony formation of plant-pathogenic bacteria, including Clavibacter michiganensis subsp. michiganensis and Ralstonia solanacearum while also inhibiting the conidial germination of plant-pathogenic fungi including Alternaria brassicicola and Colletotrichum orbiculare. The volatile compounds were isolated from the culture filtrate of P. spadiceum, and 3,4-dichloro-4-methoxybenzaldehyde (DCMB) was identified as a major compound. DCMB significantly inhibited bacterial colonization at 10 lg ml À1 and fungal conidial germination at 0Á1-1 lg ml À1 as a vapour. Conclusions: This is the first report on the production of the volatile compound DCMB by P. spadiceum and on the antimicrobial activity of DCMB against plant-pathogenic bacteria and fungi at low concentrations. It may be possible to use the compound as an agent for protecting crops from bacterial and fungal diseases during cultivation and storage. Significance and Impact of the Study: This study provides an understanding of antimicrobial activity of the mushroom volatile compound that may be useful as a novel biological control agent for protecting various plant diseases.

Identification of two phthalide derivatives and an oxindole compound isolated from the edible mushroom <i>Pleurotus ostreatus</i> and their inhibitory activities against plant pathogenic microorganisms

“…The electrospray ionization (ESI) mass spectra (MS) were recorded using a Quattro Micro API mass spectrometer (Waters, Milford, MA, USA) connected with an Acquity UPLC (Waters). The 1 H NMR (600 MHz), 13 C NMR (150 MHz), and 2D spectra were recorded using a JEOL JNM-ECZ600 (Tokyo, Japan). Preparative high-performance liquid chromatography (HPLC) was performed using a 10A HPLC system (Shimadzu, Kyoto, Japan).…”

“…Compound 1 H NMR (600 MHz, CDCl 3 ): δ H 6.92 (d, J=1.8 Hz); 6.67 (d, J=1.8 Hz, H); 5.19 (s, 2H); 3.86 (s, 3H); 3.85 (s, 3H). 13 C NMR (150 MHz, CDCl 3 ): δ C 171.4 (C1); 162.6 (C6); 154.9 (C4); 128.4 (C8); 128 (C9); 104.9 (C5); 98.6 (C7); 68.2 (C3); 56.0 (C10); 55.7 (C11).…”

“…Compound 13 C NMR (150 MHz, CDCl 3 ): δ C 180.0 (C2); 139.9 (C9); 131.6 (C4); 129.9 (C7); 124.0 (C5); 123.4 (C6); 110.1 (C8); 73.8 (C3); 24.9 (C10).…”

A Review on Microbial Products and Their Perspective Application as Antimicrobial Agents

“…Hamamoto et al [ 164 ] screened the volatile compound, 3,4-dichloro-4-methoxy benzaldehyde (DCMB) from mycelia of Porostereum spadiceum. It remarkably inhibited the plant-pathogenic bacteria ( Clavibacter michiganensis and Ralstonia solanacearum ) and inhibited the conidial germination of plant-pathogenic fungi ( Alternaria brassicicola and Colletotrichum orbiculare ).…”

A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents

“…However, further studies are required to fortify and test these extracts and NPs against human and plant pathogenic microbes coupled with the purification and characterization of the compounds from mushroom. Hamamoto et al [158] screened the volatile compound, 3,4-dichloro-4-methoxy benzaldehyde (DCMB) from mycelia of Porostereum spadiceum. It remarkably inhibited the plant-pathogenic bacteria (Clavibacter michiganensis and Ralstonia solanacearum) and inhibit the conidial germination of plant-pathogenic fungi (Alternaria brassicicola and Colletotrichum orbiculare).…”