Antimicrobial antibodies by phage display: Identification of antibody-based inhibitor against mycobacterium tuberculosis isocitrate lyase

Ch’ng, Angela Chiew Wen; Schepergerdes, Lena; Choong, Yee Siew; Hust, Michael; Lim, Theam Soon

doi:10.1016/j.molimm.2022.08.005

Cited by 4 publications

(1 citation statement)

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“…It is postulated that the inhibition of ICL can disrupt the life cycle of MTB. Theam Soon Lim et al panned against MTB ICL proteins using a previously developed naive human scFv antibody phage library and identified a single scFv clone (a-rICL-C6) against MTB ICL [ 30 , 63 ]. Further study showed that the a-rICL-C6 monoclonal antibody was able to relieve recombinant ICL enzyme activity with an estimated IC50 of 10 mg∙mL −1 .…”

Section: Application Of Phage Display In the Antibacterial Fieldmentioning

confidence: 99%

Phage Display-Derived Peptides and Antibodies for Bacterial Infectious Diseases Therapy and Diagnosis

Zhao

Nie

et al. 2023

Molecules

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The emergence of antibiotic-resistant-bacteria is a serious public health threat, which prompts us to speed up the discovery of novel antibacterial agents. Phage display technology has great potential to screen peptides or antibodies with high binding capacities for a wide range of targets. This property is significant in the rapid search for new antibacterial agents for the control of bacterial resistance. In this paper, we not only summarized the recent progress of phage display for the discovery of novel therapeutic agents, identification of action sites of bacterial target proteins, and rapid detection of different pathogens, but also discussed several problems of this technology that must be solved. Breakthrough in these problems may further promote the development and application of phage display technology in the biomedical field in the future.

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Section: Application Of Phage Display In the Antibacterial Fieldmentioning

confidence: 99%