Antimicrobial resistance in extended spectrum -lactamases (ESBL)-producing Escherichia coli isolated from human urinary tract infections in Ndjamena, Chad

Ndoutamia, Guelmbaye; Yandai, Fissou Henry; Nadlaou, Bessimbaye

doi:10.5897/ajmr2014.7338

Cited by 3 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This practice could contribute to the selection of multi-resistant strains. The results of this study are comparable to those of Nadmi et al (2010), Ahmed et al (2012), Ndoutamia et al (2014), Sumera et al (2014) and Dissinviel et al (2017). Isolated strains in hospitalized patients were more resistant than those detected in outpatients.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and resistance profile of Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in N'Djamena, Tchad

Yandai¹,

Ndoutamia²,

Nadlaou³

et al. 2019

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Bacterial resistance to antibiotics is a worldwide problem. In Chad, statistical data are scarce. The reason why this study was undertaken from June 2014 to December 2016, to identify the main Enterobacteriaceae responsible for urinary tract infections and their susceptibility to antibiotics. Germs were isolated and identified by standard microbiology methods and tested with antibiotics according to Kirby-Bauer technique. Data collected was analyzed using Excel and Statistical Package for Social Sciences (SPSS) version18.0. Out of the 503 urine samples analyzed, 93 Enterobacteriaceae were isolated (18.5%) of which 60 (64.5%) were Escherichia coli, 23 (24.73%) Klebsiella pneumoniae and 10 (10.75%) were other Enterobacteriaceae. Bacterial resistances to the following were observed: amoxicilline (96.66%-100%), cefoxitine (13.33%-30.4%), cefotaxime (33.3%-56.52%), gentamycine (28.33%-39.13%), and nalidixic acid (31.66%-43.47%), trimethoprim-sulfamethoxazole (65%-95.65%) and fosfomycine (8.33%-13.04%). The present study identified two bacteria associated with urinary tract infections and their resistances to antibiotics commonly used in Chad. It is important to rationalize the use of antibiotics that have good antibacterial activity. Diversified studies in human and veterinary medicine are needed to better control the emergence of new resistance in N'Djamena.

show abstract

Section: Discussionsupporting

confidence: 89%

Prevalence and resistance profile of Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in N'Djamena, Tchad

Yandai¹,

Ndoutamia²,

Nadlaou³

et al. 2019

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

show abstract

Review and mapping of carbapenem-resistant Enterobacteriaceae in Africa: Using diverse data to inform surveillance gaps

Mitgang

Hartley

Malchione

et al. 2018

International Journal of Antimicrobial Agents

View full text Add to dashboard Cite

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

Mahamat

Tidjani

Lounnas

et al. 2019

Antimicrob Resist Infect Control

View full text Add to dashboard Cite

BackgroundFecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad.MethodsIn total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method.ResultsESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored blaCTX-M genes: blaCTX-M-15 in 94.25% (82/87) and blaCTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains.ConclusionsThe high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria.

show abstract

Antimicrobial resistance in extended spectrum -lactamases (ESBL)-producing Escherichia coli isolated from human urinary tract infections in Ndjamena, Chad

Cited by 3 publications

References 19 publications

Prevalence and resistance profile of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> isolated from urinary tract infections in N'Djamena, Tchad

Prevalence and resistance profile of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> isolated from urinary tract infections in N'Djamena, Tchad

Review and mapping of carbapenem-resistant Enterobacteriaceae in Africa: Using diverse data to inform surveillance gaps

Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

Contact Info

Product

Resources

About