Antimicrobial Susceptibility of <i>Escherichia coli</i> Strains Isolated from <i>Alouatta</i> spp. Feces to Essential Oils

Lara, Valéria Maria; Carregaro, Adriano Bonfim; Santurio, Deise Flores; Sá, Mariangela Facco de; Santurio, Jânio M.; Alves, Sydney Hartz

doi:10.1155/2016/1643762

Cited by 20 publications

(20 citation statements)

References 25 publications

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“…patterns were found for both species investigated in this study. The concentrations found are in accordance to previous studies (Burt et al, 2016;Pei, Zhou, Ji, & Xu, 2009) and were lower than the ones found for others essential oils (oregano, thyme and clove) tested against the same species (Lara et al, 2016;Solarte et al, 2018). Furthermore, MIC and MBC values almost coincided (two-fold difference), which indicates that CNMA killing is generally bactericidal, a highly desirable mode of action to control microbial contamination (Ocampo et al, 2014).…”

Section: Antimicrobial Activity Of Cnma Towards Planktonic Cultures Osupporting

confidence: 90%

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Alves

Cerqueira

Pastrana

et al. 2020

Food Research International

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Notwithstanding the implementation of good processing practices in food companies and appropriate washing of food products by the consumer, Salmonella and Escherichia coli outbreaks continue to occur. In this study, different combinations of bacteriophages (phages) and cinnamaldehyde (CNMA) were incorporated on sodium alginate emulsion-based films to impart them with antimicrobial activity towards S. Enteritidis and E. coli. Films were prepared by casting and they were characterized in terms of CNMA and/or phages loading, thickness, moisture content, water vapor permeability (WVP), swelling index (SW), chemical interactions by FTIR, surface morphology by SEM and antimicrobial performance. Results showed that phages incorporation was not compromised by CNMA as evidenced by their viability inside the films. Increasing CNMA concentration yielded formulations less heterogeneous and a higher amount of CNMA loaded. Films characterization revealed that, in general, phages incorporation did not introduce significant changes on films parameters while the presence of CNMA increased the roughness, thickness and swelling ability of films. Sodium alginate films incorporated with EC4 and φ135 phages displayed antimicrobial activity against E. coli and S. Enteritidis, respectively, while CNMA empowered the films with activity against both species. Combination of both phages with the higher concentration of CNMA resulted in a synergic antimicrobial effect against E. coli and a facilitative effect against Salmonella. Overall, incorporation of EC4 and φ135 phages together with CNMA on alginate emulsion-based films holds great potential to be further applied in food packaging to prevent food contamination.

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Section: Antimicrobial Activity Of Cnma Towards Planktonic Cultures Osupporting

confidence: 90%

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Alves

Cerqueira

Pastrana

et al. 2020

Food Research International

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“…The second stage. E. coli resistance test against some antibiotics were used dilution method, namely Minimum Inhibitory Concentration (MIC) 20 and Minimum Bacteriocide Concentration (MBC). The test were done through several antibiotics: amoxicillin, amphicillin, erythromycin, cephalosporins, tetracycline, cloxacilin and gentamicin.…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of Meniran (Phyllanthus Niruri Linn) as Antibacterial for Resistance Antibiotics Prevention of Enterotoxin Escherichia Coli

Hidanah

Sabdoningrum

Wahyuni

et al. 2018

IJTID

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Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which 21% showed resistance to various antibiotics, such as E. coli enterotoxin. Alternative efforts were needed as a substitute for antibiotics, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The research was done through several stages: isolation and identification of E. coli enterotoxin from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against E. coli enterotoxin The study was divided into five treatments: T0+ (group of chickens were infected by E. coli enterotoxin), T0- (control group, not infected), T1 (infected by E. coli enterotoksin + 20% meniran extract), T2 (infected by E. coli enterotoksin + 25% extract meniran), T3 (infected by E. coli enterotoxin + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results were showed that all of E. coli DNA isolates which tested by the PCR method was showed positive reactions at 600 bp. In the next stage, that E. coli enterotoxin are resistance to some antibiotics, such as Amoxicillin, Amphicillin, Erythromycin, Cephalosporins, Tetracycline, Cloxacillin and Gentamicin. Furthermore, 30% Phyllanthus niruri linn extract effective as an antibacterial for the prevention of antibiotic resistance from E. coli enterotoxin.

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“…Reports have also suggested the antioxidative and anticancer activities of lemongrass as well (5, 6, 12-14, 22, 40, 44). Several others also indicated that lemongrass essential oil (LGEO) shows potent antibacterial activity on several microorganisms, including E. coli, Sterptococus pyonenes, and Pseudomonas fluorescence (10,23,25,26,37,50,56,58). In this study, we investigated anti-NoV activity of LGEO both in vitro and in vivo.…”

mentioning

confidence: 97%

Inactivation of Norovirus by Lemongrass Essential Oil Using a Norovirus Surrogate System

Kim

You

Lee

et al. 2017

Journal of Food Protection

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This study investigated the effect of lemongrass essential oil (LGEO) on the infectivity and viral replication of norovirus. Murine norovirus 1 (MNV-1), a surrogate of human norovirus, was preincubated with LGEO and then used to infect RAW 264.7 cells in a plaque reduction assay. LGEO exhibited a significant reduction in MNV-1 plaque formation in both time- and dose-dependent manners. The quantification of viral genome by quantitative real-time PCR showed similar results in line with those of the plaque reduction assay. It was revealed that citral, a single compound in LGEO, showed dramatic reduction in MNV-1 infectivity (-73.09% when using a treatment of 0.02%, v/v). The inhibitory activity of LGEO on viral replication was further investigated in HG23 cells that harbored a human norovirus replicon. LGEO treatment significantly reduced viral replication in HG23 cells, which suggests that LGEO may have dual inhibitory activities that inactivate viral coat proteins required for viral infection and suppress norovirus genome replication in host cells. In animal experiments, oral administration of murine norovirus preincubated with LGEO significantly suppressed virus infectivity in vivo. Collectively, these results suggest that LGEO, in particular the LGEO component citral, inactivates the norovirus and its subsequent replication in host cells. Thus, LGEO shows promise as a method of inhibiting norovirus within the food industry.

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Antimicrobial Susceptibility of Escherichia coli Strains Isolated from Alouatta spp. Feces to Essential Oils

Cited by 20 publications

References 25 publications

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Effectiveness of Meniran (Phyllanthus Niruri Linn) as Antibacterial for Resistance Antibiotics Prevention of Enterotoxin Escherichia Coli

Inactivation of Norovirus by Lemongrass Essential Oil Using a Norovirus Surrogate System

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