Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

Bhattacharya, Udayan; Adak, Shanta; Majumder, Niladri Shekhar; Bera, Biswajit; Giri, Ashok K.

doi:10.1186/1472-6882-14-327

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Tea leaves can be processed into the most widely consumed beverage. Increasing evidence suggests that tea extracts produce beneficial health effects, such as anticancer [ 2 ], anti-vascular disease [ 3 ], anti-bacterial [ 4 ], anti-inflammation [ 5 ], and anti-allergic [ 6 ] activities. The leaves of tea plants have large amounts of flavonoids, which include flavones, flavanones, flavonols, flavan 3-ols (as known as catechins), and anthocyanidins [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)

Zhou

et al. 2016

IJMS

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Tea leaves contain abundant flavan-3-ols, which include dihydroxylated and trihydroxylated catechins. Flavonoid 3′-hydroxylase (F3′H: EC 1.14.13.21) is one of the enzymes in the establishment of the hydroxylation pattern. A gene encoding F3′H, designated as CsF3′H, was isolated from Camellia sinensis with a homology-based cloning technique and deposited in the GenBank (GenBank ID: KT180309). Bioinformatic analysis revealed that CsF3′H was highly homologous with the characterized F3′Hs from other plant species. Four conserved cytochrome P450-featured motifs and three F3′H-specific conserved motifs were discovered in the protein sequence of CsF3′H. Enzymatic analysis of the heterologously expressed CsF3′H in yeast demonstrated that tea F3′H catalyzed the 3′-hydroxylation of naringenin, dihydrokaempferol and kaempferol. Apparent Km values for these substrates were 17.08, 143.64 and 68.06 μM, and their apparent Vmax values were 0.98, 0.19 and 0.44 pM·min−1, respectively. Transcription level of CsF3′H in the new shoots, during tea seed germination was measured, along with that of other key genes for flavonoid biosynthesis using real-time PCR technique. The changes in 3′,4′-flavan-3-ols, 3′,4′,5′-flavan-3-ols and flavan-3-ols, were consistent with the expression level of CsF3′H and other related genes in the leaves. In the study of nitrogen supply for the tea plant growth, our results showed the expression level of CsF3′H and all other tested genes increased in response to nitrogen depletion after 12 days of treatment, in agreement with a corresponding increase in 3′,4′-catechins, 3′,4′,5′-catechins and flavan 3-ols content in the leaves. All these results suggest the importance of CsF3′H in the biosynthesis of 3′,4′-catechins, 3′,4′,5′-catechins and flavan 3-ols in tea leaves.

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Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)

Zhou

et al. 2016

IJMS

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“…Actually, a growing number of studies have identified different plants extracts with antigenotoxic potential, such as: Mentha longifolia (Al-Ali et al 2014), Dioscorea pentaphylla (Prakash et al 2014), Lilium candidum (Jovtchev et al 2014), Eucalyptus Gunnii (Bugarin et al 2014), Camellia sinensis (Bhattacharya et al 2014), Curcuma longa (Liju et al 2014), Celtis iguanaea (Borges et al 2013), Solanum paniculatum (Vieira et al 2013), Synadenium umbellatum (Melo-Reis et al 2011), Ginkgo biloba (Vilar et al 2009), and others. Thus, there has been growing interest in finding and using natural plant products to reduce genotoxic and/or carcinogenic effects.…”

Section: Introductionmentioning

confidence: 99%

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes

Chaves

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct-and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/ kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow. Regarding the antimutagenic effect, all doses of C. guianensis oil were significantly (p < 0.05) effective in reducing the frequency of micronucleated polychromatic erythrocytes, when compared with MMC or CP alone. Based on these results, our results suggest that the C. guianensis oil shows medicinal potential as an antimutagenic agent, modulating the mutagenicity caused by both direct-and indirect-acting chemical mutagens, in a mammalian model.

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Antimutagenic and anticlastogenic effects of Turkish Black Tea on TA98 and TA100 strains of Salmonella typhimurium ( in vitro ) and mice ( in vivo )

Charehsaz

Sipahi

Giri

et al. 2017

Pharmaceutical Biology

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Context: Black tea has been reported to have significant antimutagenic and anticarcinogenic properties associated with its polyphenols theaflavins (TF) and thearubigins (TR). Similarly, Turkish black tea (TBT) also contains a considerable amount of TF and TR.Objective: This study investigated the mutagenic, antimutagenic and anticlastogenic properties of TBT.Materials and methods: The mutagenic and antimutagenic effects of TBT (10 to 40000 μg/plate) were investigated in vitro on Salmonella strains TA98 and TA100 with and without S9 fraction. Anticlastogenic effect was studied at concentrations of 300–1200 mg/kg TBT extract by chromosomal aberrations (CA) assay from bone marrow of mice.Results: The results of this study did not reveal any mutagenic properties of TBT. On the contrary, TBT extract exhibited antimutagenic activity at >1000 μg/plate concentrations in TA98 strain with and without S9 activation (40% inhibition with S9 and 27% without S9). In TA100 strain, the antimutagenic activity was observed at >20,000 μg/plate TBT extracts without S9 activation (28% inhibition) and at >1000 μg/plate with S9 activation (59% inhibition). A significant decrease in the percentage of aberrant cells (12.33% ± 1.27) was observed in dimethylbenz(a)anthracene (DMBA) plus highest concentration (1200 mg/kg) of TBT extract-treated group when compared to only DMBA-treated group (17.00% ± 2.28).Discussion and conclusion: Results indicated that TBT can be considered as genotoxically safe, because it did not exert any mutagenic and clastogenic effects. As a result, TBT exhibited antimutagenic effects more apparently after metabolic activation in bacterial test system and had an anticlastogenic effect in mice.

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Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

Cited by 4 publications

References 26 publications

Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)

Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Antimutagenic and anticlastogenic effects of Turkish Black Tea on TA98 and TA100 strains of Salmonella typhimurium ( in vitro ) and mice ( in vivo )

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