Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

Abstract

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

Formulation and Evaluation of Gambier (Uncaria gambir)-Chitosan Microparticle Intranasal Delivery for Alzheimer’s Diseases

“…One mL of DPPH solution 0.3 mM was taken and added with 2.5 mL of the sample from each concentration, vortexed and allowed for 30 minutes in the dark room. The observed color changes and absorption were measured using a UV-Vis spectrophotometer with maximum DPPH wavelength measured at 517 nm (Fithri et al, 2019) . Antioxidant activity of the samples was then determined by the amount of DPPH radical uptake resistance by calculating the percentage of DPPH inhibition activity (Equation 3).…”

Bioactive Compounds of Petai Beans (Parkia speciosa Hassk.)

“…The naturally occurring phenolic compounds contain two carbon frames: hydroxycinnamic and hydroxybenzoic structure frames and other major fraction of carbon structures were ether, ester, or acetals [16]. P. speciosa sample were prepared by drying seeds or pods by using vacuum or freeze drying, then grind and homogenized in small size particles before extraction of phytochemicals [17]. After preparing the dry samples of P. speciosa, extract of active compounds by using organic solvents such as ethanol, methanol, ethyl acetate, petroleum ether, and hydrosolvents was prepared.…”

Sequential Microwave-Ultrasound Assisted Extraction of Flavonoid from <i>Moringa oleifera</i>: Product Characteristic, Antioxidant and Antibacterial Activity

“…As a result, the cell swells, stretches, and ruptures, allowing the metabolic component to leave and be extracted by the solvent [9]. UAE and MAE can be considered as one of the most effective extraction strategies for extracting bioactive chemicals from plant materials, such as flowers, due to their high extraction efficiency [10], fruits [11], leaves [5], bark [12], seeds [13], and pods [14].…”