Antioxidant and cytotoxic studies for kaempferol, quercetin and isoquercitrin

Vellosa, José Carlos Rebuglio; Regasini, Luís Octávio; Khalil, Najeh Maissar; Bolzani, Vanderlan da Silva; Khalil, Omar Arafat Kdudsi; Manente, Francine Alessandra; Netto, Harli Pasquini; Oliveira, Olga Maria Mascarenhas de Faria

doi:10.1590/s0100-46702011000200001

Cited by 69 publications

(52 citation statements)

References 27 publications

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“…Quercetin was the more efficient agent against HOCl possessing a low IC 50 (0.0008 mM). Isoquercitrin and kaempferol had similar potential as scavengers of HOCl, respectively 0.0018 and 0.0020 mM IC 50 values (Vellosa et al 2011).…”

Section: Resultsmentioning

confidence: 90%

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Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species

et al. 2014

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Biological activities of flavonoids have been extensively reviewed in literature. The biochemical profile of afzelin, kaempferitrin, and pterogynoside acting on reactive oxygen species was investigated in this paper. The flavonoids were able to act as scavengers of the superoxide anion, hypochlorous acid and taurine chloramine. Although flavonoids are naturally occurring substances in plants which antioxidant activities have been widely advertised as beneficial, afzelin, kaempferitrin, and pterogynoside were able to promote cytotoxic effect. In red blood cells this toxicity was enhanced, depending on flavonoids concentration, in the presence of hypochlorous acid, but reduced in the presence of 2,2'-azo-bis(2-amidinopropane) free radical. These flavonoids had also promoted the death of neutrophils, which was exacerbated when the oxidative burst was initiated by phorbol miristate acetate. Therefore, despite their well-known scavenging action toward free radicals and oxidants, these compounds could be very harmful to living organisms through their action over erythrocytes and neutrophils.

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Section: Resultsmentioning

confidence: 90%

“…Those samples were effective superoxide scavengers. It had been observed that quercetin (IC 50 = 0.025 mM) was a powerful superoxide scavenger agent followed by isoquercitrin (IC 50 = 0.111 mM) and kaempferol (IC 50 = 0.243 mM) (Vellosa et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species

et al. 2014

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“…In vitro studies comparing the antioxidant effect of flavonols (glycosides and aglycones) confirmed that their protective efficacy against lipid peroxidation reaction were mediated by free radicalscavenging activities [3,7] . However, it has been reported that these substances may also be cytotoxic and damage the cell membrane (mainly erythrocytes) in the presence of ROS, and thereby become extremely harmful to living organisms them selves [28] . Boligon et al [29] disagreed with this study and, therefore, analyzed the protective effects of flavonoids against chromosome damage induced by hydrogen peroxide (H2O2) n human lymphocytes.…”

Section: Effects Of Iq In Cancermentioning

confidence: 99%

Review of anticancer mechanisms of isoquercitin

Orfali¹,

Duarte²,

Bonadio³

et al. 2016

WJCO

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This review was based on a literature search of PubMed and Scielo databases using the keywords "quercetin, rutin, isoquercitrin, isoquercitin (IQ), quercetin-3-glucoside, bioavailability, flavonols and favonoids, and cancer" and combinations of all the words. We collected relevant scientific publications from 1990 to 2015 about the absorption, bioavailability, chemoprevention activity, and treatment effects as well as the underlying anticancer mechanisms of isoquercitin. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. The subclass of flavonols receives special attention owing to their health benefits. The main components of this class are quercetin, rutin, and IQ, which is a flavonoid and although mostly found as a glycoside, is an aglycone (lacks a glycoside side chain). This compound presents similar therapeutic profiles to quercetin but with superior bioavailability, resulting in increased efficacy compared to the aglycone form. IQ has therapeutic applications owing to its wide range of pharmacological effects including antioxidant, antiproliferative, anti-inflammatory, anti-hypertensive, and anti-diabetic. The protective effects of IQ in cancer may be due to actions on lipid peroxidation. In addition, the antitumor effect of IQ and its underlying mechanism are related to interactions with Wnt signaling pathway, mixed-lineage protein kinase 3, mitogen-activated protein kinase, apoptotic pathways, as well proinflammatory protein signaling. This review contributed to clarifying the mechanisms of absorption, metabolism, and actions of IQ and isoquercitrin in cancer.

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“…The structures for kaempferol and quercetin were given [ Figure 11]. 47 In the present study, two of the phytochemicals namely kaempferol and quercetin were analyzed by TLC and HPLC methods. The R f values noted for kaempferol was 0.47 and for quercetin, 0.62 after examining the yellow fluorescent spots under UV.…”

Section: Resultsmentioning

confidence: 99%

Detection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC

Mamillapalli¹,

Rahaman²,

Rani³

2018

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Back ground: High-performance Liquid Chromatography is one of the major analytical techniques used in the quality control of phytochemicals. Objective: This research article presents the development of HPLC method to detect and quantify the major marker components, kaempferol, and quercetin from four plant species. Materials and Methods: HPLC method was developed for the qualitative and quantitative analysis of plant extracts by using orthophosphoric acid and methanol (95:5) at 370 nm for kaempferol, methanol and orthophosphoric acid (60:40) at 262nm for quercetin. Results: Kaempferol was detected from the hydro alcoholic extracts of Talinum portulacifolium leaves (RT 13.720, concentration 1.08 mg/ml) and flowers of Gomphrena serrata (RT 13.758, concentration 2.13mg/ml). Kaempferol was reported for the first time from Gomphrena serrata. Quercetin was separated and identified from the hydro alcoholic extracts Alternanthera sessilis stems (RT 6.503, concentration 0.01mg/ml). The hydroalcoholic extract of Euphorbia heterophylla stems (RT 6.588, concentration 0.01mg/ml) was also evaluated for the presence of quercetin. Conclusion: The method developed is very useful tool for qualifying and quantifying the plant specimens as well as their extracts.

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Antioxidant and cytotoxic studies for kaempferol, quercetin and isoquercitrin

Cited by 69 publications

References 27 publications

Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species

Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species

Review of anticancer mechanisms of isoquercitin

Detection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC

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