Antioxidant and dual dose-dependent antigenotoxic and genotoxic properties of an ethanol extract of propolis

Cruz, Maria Teresa; Antunes, Paula Tavares; Paulo, L.; Ferreira, Ana; Cunha, Ana; Aguiar, Cristina Almeida; Oliveira, Rui Pedro Soares de

doi:10.1039/c6ra04856k

Cited by 15 publications

(21 citation statements)

References 42 publications

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“…Even though these ingredients are already being used in cosmetics, the lack of antigenotoxicological studies makes it crucial to investigate further how to incorporate them in cosmetics to benefit human health. Studies have shown that plants, fruits, and vegetables with antigenotoxic properties show promising results for the cosmetic industry [ 193 , 194 , 195 ]. Additional investigation can be carried out, namely, evaluating the cosmetic properties of the natural ingredients towards promoting DNA integrity.…”

Section: Discussionmentioning

confidence: 99%

Natural Ingredients Common in the Trás-os-Montes Region (Portugal) for Use in the Cosmetic Industry: A Review about Chemical Composition and Antigenotoxic Properties

Gonçalves

Gaivão

2021

Molecules

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The natural cosmetics market has grown since consumers became aware of the concept of natural-based ingredients. A significant number of cosmetics have an ecological impact on the environment and carry noxious and chemically potent substances. Thus, the use of natural and organic cosmetics becomes increasingly important since it is clear that topical treatment with cosmeceuticals can help improve skin rejuvenation. A substantial investigation into the benefits that fruits and plants can bring to health is required. Studies have shown that antigenotoxic properties are linked to anti-aging properties. Several studies have shown potential antigenotoxicity in natural ingredients such as Almonds (Prunus dulcis), Elderberry (Sambucus nigra), Olives (Olea europaea), and Grapes (Vitis vinifera). This review presents an overview of research conducted on these natural ingredients, the most common in the Northeast of Portugal. This region of Portugal possesses the most organic farmers, and ingredients are easily obtained. The Northeast of Portugal also has climatic, topographic, and pedological differences that contribute to agricultural diversity.

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Section: Discussionmentioning

confidence: 99%

Natural Ingredients Common in the Trás-os-Montes Region (Portugal) for Use in the Cosmetic Industry: A Review about Chemical Composition and Antigenotoxic Properties

Gonçalves

Gaivão

2021

Molecules

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“…In humans, propolis was widely used as a folk medicine worldwide since ancient times. Propolis is known to possess valuable biological properties (Sforcin 2016;Cornara et al 2017) such as antibacterial (Oliveira et al 2017;Roh and Kim 2018), antioxidant (Cruz et al 2016), anti-inflammatory (Moreira et al 2008), antineurodegenerative (Falca ˜o et al 2010), anti-tumor (Silva-Carvalho et al 2014, antifungal (Roh and Kim 2018), anti-protozoan (Falca ˜o et al 2014), anti-viral (Yildirim et al 2016), hepato-protective (Sheng et al 2007), local-anesthetic (Moreira et al 2008, and free-radical-scavenging (Al-Ani et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds from propolis

2019

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The aim of this study was to compare different techniques in order to achieve a high extraction of phenolic compounds from propolis. For this purpose, it was investigated the use of double maceration (24 h at room temperature with continuous agitation at 250 rpm), double microwave treatments (1 min at 140 W) and double ultrasound-assisted extraction (15 min at 20 kHz) using 70% ethanol. The extraction efficiency was measured based on extraction yield, total phenolic content, flavones and flavonol content, and flavanone and dihydroflavonol content. The ultrasonic extraction had an extraction yield higher than microwave extraction and maceration. The yield of the propolis ranged between samples and between the years of propolis harvesting. Of the twelve quantified phenolic compounds, p-coumaric acid was the most abundant (271.65 mg/g propolis).

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“…The treated and untreated cells were washed with, and resuspended in, phosphate buffer, then 10-times diluted and added to 10 μM DCFDA (Sigma-Aldrich) in 1% DMSO. DCFDA, as a lipophilic compound, permeates the metabolically active cell [ 39 ]. Once oxidized by ROS, DCFDA is converted to a highly fluorescent DCF, which fluoresces at 527 nm, and thus provides an estimate of ROS levels [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Acute Exposure to Bisphenol A Causes Oxidative Stress Induction with Mitochondrial Origin in Saccharomyces cerevisiae Cells

Ďurovcová

Goffa

Šestáková

et al. 2021

JoF

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Bisphenol A (BPA) is a major component of the most commonly used plastic products, such as disposable plastics, Tetra Paks, cans, sport protective equipment, or medical devices. Due to the accumulation of excessive amounts of plastic waste and the subsequent release of BPA into the environment, BPA is classified as a pollutant that is undesirable in the environment. To date, the most interesting finding is the ability of BPA to act as an endocrine disrupting compound due to its binding to estrogen receptors (ERs), and adverse physiological effects on living organisms may result from this action. Since evidence of the potential pro-oxidizing effects of BPA has accumulated over the last years, herein, we focus on the detection of oxidative stress and its origin following BPA exposure using pulsed-field gel electrophoresis, flow cytometry, fluorescent microscopy, and Western blot analysis. Saccharomyces cerevisiae cells served as a model system, as these cells lack ERs allowing us to dissect the ER-dependent and -independent effects of BPA. Our data show that high concentrations of BPA affect cell survival and cause increased intracellular oxidation in yeast, which is primarily generated in the mitochondrion. However, an acute BPA exposure does not lead to significant oxidative damage to DNA or proteins.

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Antioxidant and dual dose-dependent antigenotoxic and genotoxic properties of an ethanol extract of propolis

Abstract: Propolis is a resinous product made by honeybees from plant-derived materials, with high content of polyphenols associated to beneficial bioactivities with potential use as a natural food additive for preservation and as a functional food ingredient.

Cited by 15 publications

References 42 publications

Natural Ingredients Common in the Trás-os-Montes Region (Portugal) for Use in the Cosmetic Industry: A Review about Chemical Composition and Antigenotoxic Properties

Natural Ingredients Common in the Trás-os-Montes Region (Portugal) for Use in the Cosmetic Industry: A Review about Chemical Composition and Antigenotoxic Properties

Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds from propolis

Acute Exposure to Bisphenol A Causes Oxidative Stress Induction with Mitochondrial Origin in Saccharomyces cerevisiae Cells

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